MOLECULAR DETECTION OF NEW STREPTOMYCES SPP. FROM IRAQI OIL CONTAMINATED SOIL

Streptomyces spp. is one of Actinomycete that produce variety of secondary metabolite (antibacterial, antifungal and anticancer product). Bacteria that used in this study was isolated from oily soil in Baghdad /Iraq. The morphological characters was gram positive, branching, spore former. The goal...

Full description

Bibliographic Details
Main Authors: A. K. Al zaidy, A. A. Al doori, E. H. Yousif
Format: Article
Language:English
Published: Baghdad University 2023-10-01
Series:The Iraqi Journal of Agricultural science
Subjects:
Online Access:https://jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/1828
_version_ 1797647120292904960
author A. K. Al zaidy
A. A. Al doori
E. H. Yousif
author_facet A. K. Al zaidy
A. A. Al doori
E. H. Yousif
author_sort A. K. Al zaidy
collection DOAJ
description Streptomyces spp. is one of Actinomycete that produce variety of secondary metabolite (antibacterial, antifungal and anticancer product). Bacteria that used in this study was isolated from oily soil in Baghdad /Iraq. The morphological characters was gram positive, branching, spore former. The goal of this research was to find genetic polymorphisms of one bacterial isolate (assigned S1) and to assess the accurate phylogenetic distribution of this isolated based on ribosomal sequences. In this work one genomic region was amplified that covered a portion of the 16S rRNA sequences. According to the identified results, the currently investigated sample was positioned within the major phylogenetic clade of the Streptomyces albidoflavus sequences in the generated comprehensive tree. In conclusion, the present tree provided an inclusive tool for the confirmed identity of the sample under investigation As a result, the PCR-sequencing approach was used in the studied sample has presented a confirmed identity of this sample and showed the pattern of its phylogenetic distribution. This sort of observed diversity may suggest the possibility to utilize the current 16S rRNA sequences in the accurate detection and discrimination for the Streptomyces albidoflavus sequences. 
first_indexed 2024-03-11T15:11:45Z
format Article
id doaj.art-6764c8c29d5847bd95730467da3fba44
institution Directory Open Access Journal
issn 0075-0530
2410-0862
language English
last_indexed 2024-03-11T15:11:45Z
publishDate 2023-10-01
publisher Baghdad University
record_format Article
series The Iraqi Journal of Agricultural science
spelling doaj.art-6764c8c29d5847bd95730467da3fba442023-10-29T14:23:06ZengBaghdad UniversityThe Iraqi Journal of Agricultural science0075-05302410-08622023-10-0154510.36103/ijas.v54i5.1828MOLECULAR DETECTION OF NEW STREPTOMYCES SPP. FROM IRAQI OIL CONTAMINATED SOIL A. K. Al zaidyA. A. Al dooriE. H. Yousif Streptomyces spp. is one of Actinomycete that produce variety of secondary metabolite (antibacterial, antifungal and anticancer product). Bacteria that used in this study was isolated from oily soil in Baghdad /Iraq. The morphological characters was gram positive, branching, spore former. The goal of this research was to find genetic polymorphisms of one bacterial isolate (assigned S1) and to assess the accurate phylogenetic distribution of this isolated based on ribosomal sequences. In this work one genomic region was amplified that covered a portion of the 16S rRNA sequences. According to the identified results, the currently investigated sample was positioned within the major phylogenetic clade of the Streptomyces albidoflavus sequences in the generated comprehensive tree. In conclusion, the present tree provided an inclusive tool for the confirmed identity of the sample under investigation As a result, the PCR-sequencing approach was used in the studied sample has presented a confirmed identity of this sample and showed the pattern of its phylogenetic distribution. This sort of observed diversity may suggest the possibility to utilize the current 16S rRNA sequences in the accurate detection and discrimination for the Streptomyces albidoflavus sequences.  https://jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/1828Isolate, Antibacterial, Antiviral, genetic sequences
spellingShingle A. K. Al zaidy
A. A. Al doori
E. H. Yousif
MOLECULAR DETECTION OF NEW STREPTOMYCES SPP. FROM IRAQI OIL CONTAMINATED SOIL
The Iraqi Journal of Agricultural science
Isolate, Antibacterial, Antiviral, genetic sequences
title MOLECULAR DETECTION OF NEW STREPTOMYCES SPP. FROM IRAQI OIL CONTAMINATED SOIL
title_full MOLECULAR DETECTION OF NEW STREPTOMYCES SPP. FROM IRAQI OIL CONTAMINATED SOIL
title_fullStr MOLECULAR DETECTION OF NEW STREPTOMYCES SPP. FROM IRAQI OIL CONTAMINATED SOIL
title_full_unstemmed MOLECULAR DETECTION OF NEW STREPTOMYCES SPP. FROM IRAQI OIL CONTAMINATED SOIL
title_short MOLECULAR DETECTION OF NEW STREPTOMYCES SPP. FROM IRAQI OIL CONTAMINATED SOIL
title_sort molecular detection of new streptomyces spp from iraqi oil contaminated soil
topic Isolate, Antibacterial, Antiviral, genetic sequences
url https://jcoagri.uobaghdad.edu.iq/index.php/intro/article/view/1828
work_keys_str_mv AT akalzaidy moleculardetectionofnewstreptomycessppfromiraqioilcontaminatedsoil
AT aaaldoori moleculardetectionofnewstreptomycessppfromiraqioilcontaminatedsoil
AT ehyousif moleculardetectionofnewstreptomycessppfromiraqioilcontaminatedsoil