Transcriptome Analysis of Sweet Cherry (<i>Prunus avium</i> L.) Cultivar ‘Lapins’ upon Infection of <i>Pseudomonas syringae</i> pv. <i>syringae</i>
Bacterial canker caused by <i>Pseudomonas syringae</i> pv. <i>syringae</i> (Pss) is responsible for substantial loss to the production of sweet cherry in Chile. To date, the molecular mechanisms of the Pss–sweet cherry interaction and the disease-related genes in the plant ar...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/21/3718 |
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| author | Weier Cui Nicola Fiore Franco Figueroa Carlos Rubilar Lorena Pizarro Manuel Pinto Set Pérez María Francisca Beltrán Claudia Carreras Paula Pimentel Alan Zamorano |
| author_facet | Weier Cui Nicola Fiore Franco Figueroa Carlos Rubilar Lorena Pizarro Manuel Pinto Set Pérez María Francisca Beltrán Claudia Carreras Paula Pimentel Alan Zamorano |
| author_sort | Weier Cui |
| collection | DOAJ |
| description | Bacterial canker caused by <i>Pseudomonas syringae</i> pv. <i>syringae</i> (Pss) is responsible for substantial loss to the production of sweet cherry in Chile. To date, the molecular mechanisms of the Pss–sweet cherry interaction and the disease-related genes in the plant are poorly understood. In order to gain insight into these aspects, a transcriptomic analysis of the sweet cherry cultivar ‘Lapins’ for differentially expressed genes (DEGs) in response to Pss inoculation was conducted. Three Pss strains, A1M3, A1M197, and 11116_b1, were inoculated in young twigs, and RNA was extracted from tissue samples at the inoculation site and distal sections. RNA sequencing and transcriptomic expression analysis revealed that the three strains induced different patterns of responses in local and distal tissues. In the local tissues, A1M3 triggered a much more extensive response than the other two strains, enriching DEGs especially involved in photosynthesis. In the distal tissues, the three strains triggered a comparable extent of responses, among which 11116_b1 induced a group of DEGs involved in defense responses. Furthermore, tissues from various inoculations exhibited an enrichment of DEGs related to carbohydrate metabolism, terpene metabolism, and cell wall biogenesis. This study opened doors to future research on the Pss–sweet cherry interaction, immunity responses, and disease control. |
| first_indexed | 2024-03-11T11:23:25Z |
| format | Article |
| id | doaj.art-67652405d28548ce886f59dad769aa6c |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T11:23:25Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-67652405d28548ce886f59dad769aa6c2023-11-10T15:10:17ZengMDPI AGPlants2223-77472023-10-011221371810.3390/plants12213718Transcriptome Analysis of Sweet Cherry (<i>Prunus avium</i> L.) Cultivar ‘Lapins’ upon Infection of <i>Pseudomonas syringae</i> pv. <i>syringae</i>Weier Cui0Nicola Fiore1Franco Figueroa2Carlos Rubilar3Lorena Pizarro4Manuel Pinto5Set Pérez6María Francisca Beltrán7Claudia Carreras8Paula Pimentel9Alan Zamorano10Laboratorio de Fitovirología, Departamento de Sanidad Vegetal, Facultad de Ciencias Agronómicas, Universidad de Chile, Santiago 8820808, ChileLaboratorio de Fitovirología, Departamento de Sanidad Vegetal, Facultad de Ciencias Agronómicas, Universidad de Chile, Santiago 8820808, ChileLaboratorio de Inmunidad Vegetal, Instituto de Ciencias Agroalimentarias, Universidad de O’Higgins, San Fernando 3070000, ChileLaboratorio de Inmunidad Vegetal, Instituto de Ciencias Agroalimentarias, Universidad de O’Higgins, San Fernando 3070000, ChileLaboratorio de Inmunidad Vegetal, Instituto de Ciencias Agroalimentarias, Universidad de O’Higgins, San Fernando 3070000, ChileLaboratorio de Inmunidad Vegetal, Instituto de Ciencias Agroalimentarias, Universidad de O’Higgins, San Fernando 3070000, ChileLaboratorio de Patología Vegetal, Instituto de Ciencias Agroalimentarias, Animales y Ambientales, Universidad de O’Higgins, San Fernando 3070000, ChileLaboratorio de Fitovirología, Departamento de Sanidad Vegetal, Facultad de Ciencias Agronómicas, Universidad de Chile, Santiago 8820808, ChileLaboratorio de Fitovirología, Departamento de Sanidad Vegetal, Facultad de Ciencias Agronómicas, Universidad de Chile, Santiago 8820808, ChileLaboratorio de Fisiología del Estrés, Centro de Estudios Avanzados en Fruticultura (CEAF), Camino Las Parcelas, 882, Rengo 2940000, ChileLaboratorio de Fitovirología, Departamento de Sanidad Vegetal, Facultad de Ciencias Agronómicas, Universidad de Chile, Santiago 8820808, ChileBacterial canker caused by <i>Pseudomonas syringae</i> pv. <i>syringae</i> (Pss) is responsible for substantial loss to the production of sweet cherry in Chile. To date, the molecular mechanisms of the Pss–sweet cherry interaction and the disease-related genes in the plant are poorly understood. In order to gain insight into these aspects, a transcriptomic analysis of the sweet cherry cultivar ‘Lapins’ for differentially expressed genes (DEGs) in response to Pss inoculation was conducted. Three Pss strains, A1M3, A1M197, and 11116_b1, were inoculated in young twigs, and RNA was extracted from tissue samples at the inoculation site and distal sections. RNA sequencing and transcriptomic expression analysis revealed that the three strains induced different patterns of responses in local and distal tissues. In the local tissues, A1M3 triggered a much more extensive response than the other two strains, enriching DEGs especially involved in photosynthesis. In the distal tissues, the three strains triggered a comparable extent of responses, among which 11116_b1 induced a group of DEGs involved in defense responses. Furthermore, tissues from various inoculations exhibited an enrichment of DEGs related to carbohydrate metabolism, terpene metabolism, and cell wall biogenesis. This study opened doors to future research on the Pss–sweet cherry interaction, immunity responses, and disease control.https://www.mdpi.com/2223-7747/12/21/3718bacterial cankerdifferentially expressed genesGO enrichmentdisease-related geneslocal responsesdistal responses |
| spellingShingle | Weier Cui Nicola Fiore Franco Figueroa Carlos Rubilar Lorena Pizarro Manuel Pinto Set Pérez María Francisca Beltrán Claudia Carreras Paula Pimentel Alan Zamorano Transcriptome Analysis of Sweet Cherry (<i>Prunus avium</i> L.) Cultivar ‘Lapins’ upon Infection of <i>Pseudomonas syringae</i> pv. <i>syringae</i> Plants bacterial canker differentially expressed genes GO enrichment disease-related genes local responses distal responses |
| title | Transcriptome Analysis of Sweet Cherry (<i>Prunus avium</i> L.) Cultivar ‘Lapins’ upon Infection of <i>Pseudomonas syringae</i> pv. <i>syringae</i> |
| title_full | Transcriptome Analysis of Sweet Cherry (<i>Prunus avium</i> L.) Cultivar ‘Lapins’ upon Infection of <i>Pseudomonas syringae</i> pv. <i>syringae</i> |
| title_fullStr | Transcriptome Analysis of Sweet Cherry (<i>Prunus avium</i> L.) Cultivar ‘Lapins’ upon Infection of <i>Pseudomonas syringae</i> pv. <i>syringae</i> |
| title_full_unstemmed | Transcriptome Analysis of Sweet Cherry (<i>Prunus avium</i> L.) Cultivar ‘Lapins’ upon Infection of <i>Pseudomonas syringae</i> pv. <i>syringae</i> |
| title_short | Transcriptome Analysis of Sweet Cherry (<i>Prunus avium</i> L.) Cultivar ‘Lapins’ upon Infection of <i>Pseudomonas syringae</i> pv. <i>syringae</i> |
| title_sort | transcriptome analysis of sweet cherry i prunus avium i l cultivar lapins upon infection of i pseudomonas syringae i pv i syringae i |
| topic | bacterial canker differentially expressed genes GO enrichment disease-related genes local responses distal responses |
| url | https://www.mdpi.com/2223-7747/12/21/3718 |
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