Live calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies
Abstract Background The mosquito Aedes aegypti has a wide variety of sensory pathways that have supported its success as a species as well as a highly competent vector of numerous debilitating infectious pathogens. Investigations into mosquito sensory systems and their effects on behavior are valuab...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-06-01
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| Series: | BMC Neuroscience |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12868-019-0511-y |
| _version_ | 1819229822253006848 |
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| author | Michelle Bui Jennifer Shyong Eleanor K. Lutz Ting Yang Ming Li Kenneth Truong Ryan Arvidson Anna Buchman Jeffrey A. Riffell Omar S. Akbari |
| author_facet | Michelle Bui Jennifer Shyong Eleanor K. Lutz Ting Yang Ming Li Kenneth Truong Ryan Arvidson Anna Buchman Jeffrey A. Riffell Omar S. Akbari |
| author_sort | Michelle Bui |
| collection | DOAJ |
| description | Abstract Background The mosquito Aedes aegypti has a wide variety of sensory pathways that have supported its success as a species as well as a highly competent vector of numerous debilitating infectious pathogens. Investigations into mosquito sensory systems and their effects on behavior are valuable resources for the advancement of mosquito control strategies. Numerous studies have elucidated key aspects of mosquito sensory systems, however there remains critical gaps within the field. In particular, compared to that of the adult form, there has been a lack of studies directed towards the immature life stages. Additionally, although numerous studies have pinpointed specific sensory receptors as well as responding motor outputs, there has been a lack of studies able to monitor both concurrently. Results To begin filling aforementioned gaps, here we engineered Ae. aegypti to ubiquitously express a genetically encoded calcium indicator, GCaMP6s. Using this strain, combined with advanced microscopy, we simultaneously measured live stimulus-evoked calcium responses in both neuronal and muscle cells with a wide spatial range and resolution. Conclusions By coupling in vivo live calcium imaging with behavioral assays we were able to gain functional insights into how stimulus-evoked neural and muscle activities are represented, modulated, and transformed in mosquito larvae enabling us to elucidate mosquito sensorimotor properties important for life-history-specific foraging strategies. |
| first_indexed | 2024-12-23T11:19:17Z |
| format | Article |
| id | doaj.art-67675cc0bce84e13bc1f4c4565180fc4 |
| institution | Directory Open Access Journal |
| issn | 1471-2202 |
| language | English |
| last_indexed | 2024-12-23T11:19:17Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neuroscience |
| spelling | doaj.art-67675cc0bce84e13bc1f4c4565180fc42022-12-21T17:49:07ZengBMCBMC Neuroscience1471-22022019-06-0120111710.1186/s12868-019-0511-yLive calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategiesMichelle Bui0Jennifer Shyong1Eleanor K. Lutz2Ting Yang3Ming Li4Kenneth Truong5Ryan Arvidson6Anna Buchman7Jeffrey A. Riffell8Omar S. Akbari9Section of Cell and Developmental Biology, University of California, San DiegoDepartment of Entomology and Riverside Center for Disease Vector Research, Institute for Integrative Genome Biology, University of California, RiversideDepartment of Biology, University of WashingtonSection of Cell and Developmental Biology, University of California, San DiegoSection of Cell and Developmental Biology, University of California, San DiegoDepartment of Entomology and Riverside Center for Disease Vector Research, Institute for Integrative Genome Biology, University of California, RiversideDepartment of Entomology and Riverside Center for Disease Vector Research, Institute for Integrative Genome Biology, University of California, RiversideSection of Cell and Developmental Biology, University of California, San DiegoDepartment of Biology, University of WashingtonSection of Cell and Developmental Biology, University of California, San DiegoAbstract Background The mosquito Aedes aegypti has a wide variety of sensory pathways that have supported its success as a species as well as a highly competent vector of numerous debilitating infectious pathogens. Investigations into mosquito sensory systems and their effects on behavior are valuable resources for the advancement of mosquito control strategies. Numerous studies have elucidated key aspects of mosquito sensory systems, however there remains critical gaps within the field. In particular, compared to that of the adult form, there has been a lack of studies directed towards the immature life stages. Additionally, although numerous studies have pinpointed specific sensory receptors as well as responding motor outputs, there has been a lack of studies able to monitor both concurrently. Results To begin filling aforementioned gaps, here we engineered Ae. aegypti to ubiquitously express a genetically encoded calcium indicator, GCaMP6s. Using this strain, combined with advanced microscopy, we simultaneously measured live stimulus-evoked calcium responses in both neuronal and muscle cells with a wide spatial range and resolution. Conclusions By coupling in vivo live calcium imaging with behavioral assays we were able to gain functional insights into how stimulus-evoked neural and muscle activities are represented, modulated, and transformed in mosquito larvae enabling us to elucidate mosquito sensorimotor properties important for life-history-specific foraging strategies.http://link.springer.com/article/10.1186/s12868-019-0511-yGCaMP6sGECIAedes aegyptiCalciumNeuronalStimuli-evoked responses |
| spellingShingle | Michelle Bui Jennifer Shyong Eleanor K. Lutz Ting Yang Ming Li Kenneth Truong Ryan Arvidson Anna Buchman Jeffrey A. Riffell Omar S. Akbari Live calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies BMC Neuroscience GCaMP6s GECI Aedes aegypti Calcium Neuronal Stimuli-evoked responses |
| title | Live calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies |
| title_full | Live calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies |
| title_fullStr | Live calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies |
| title_full_unstemmed | Live calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies |
| title_short | Live calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies |
| title_sort | live calcium imaging of aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life history specific foraging strategies |
| topic | GCaMP6s GECI Aedes aegypti Calcium Neuronal Stimuli-evoked responses |
| url | http://link.springer.com/article/10.1186/s12868-019-0511-y |
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