Mulberry Leaf Extract Improves Metabolic Syndrome by Alleviating Lipid Accumulation In Vitro and In Vivo
Metabolic syndrome (MS) is a metabolic disease with multiple complications. Mulberry leaf extract (MLE) is rich in flavonoids and has great potential in alleviating glucose and lipid metabolism disorders. This study evaluated the effect and mechanism of MLE on the alleviation of MS. The components o...
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MDPI AG
2022-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/16/5111 |
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| author | Liangyu He Yan Xing Xinxiu Ren Mengjiao Zheng Shiqiang Yu Yinbo Wang Zhilong Xiu Yuesheng Dong |
| author_facet | Liangyu He Yan Xing Xinxiu Ren Mengjiao Zheng Shiqiang Yu Yinbo Wang Zhilong Xiu Yuesheng Dong |
| author_sort | Liangyu He |
| collection | DOAJ |
| description | Metabolic syndrome (MS) is a metabolic disease with multiple complications. Mulberry leaf extract (MLE) is rich in flavonoids and has great potential in alleviating glucose and lipid metabolism disorders. This study evaluated the effect and mechanism of MLE on the alleviation of MS. The components of the MLE were analyzed, and then the regulation of lipid metabolism by MLE in vitro and in vivo was determined. In a hepatocyte model of oleic acid-induced lipid accumulation, it was found that MLE alleviated lipid accumulation and decreased the expression of genes involved in lipogenesis. Furthermore, MLE improved obesity, insulin resistance, plasma lipid profile, and liver function in MS mice after a 15-week intervention. MLE decreased the expression of SREBP1, ACC, and FAS through the AMPK signaling pathway to inhibit lipid synthesis and increase the level of CPT1A to promote lipid decomposition to achieve its hypolipidemic effect. Meanwhile, MLE was also shown to affect the composition of the gut microbiota and the production of short-chain fatty acids, which contributed to the alleviation of lipid accumulation. Our results suggest that MLE can improve MS by improving lipid metabolism through multiple mechanisms and can be developed into dietary supplements for the improvement of MS. |
| first_indexed | 2024-03-09T12:51:57Z |
| format | Article |
| id | doaj.art-67690c1326d84ea6ac0671c4fb69d8d2 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T12:51:57Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-67690c1326d84ea6ac0671c4fb69d8d22023-11-30T22:04:20ZengMDPI AGMolecules1420-30492022-08-012716511110.3390/molecules27165111Mulberry Leaf Extract Improves Metabolic Syndrome by Alleviating Lipid Accumulation In Vitro and In VivoLiangyu He0Yan Xing1Xinxiu Ren2Mengjiao Zheng3Shiqiang Yu4Yinbo Wang5Zhilong Xiu6Yuesheng Dong7School of Bioengineering, Dalian University of Technology, Dalian 116024, ChinaSchool of Bioengineering, Dalian University of Technology, Dalian 116024, ChinaSchool of Bioengineering, Dalian University of Technology, Dalian 116024, ChinaSchool of Bioengineering, Dalian University of Technology, Dalian 116024, ChinaDianxi Research Institute, Dalian University of Technology, Baoshan 678000, ChinaDianxi Research Institute, Dalian University of Technology, Baoshan 678000, ChinaSchool of Bioengineering, Dalian University of Technology, Dalian 116024, ChinaSchool of Bioengineering, Dalian University of Technology, Dalian 116024, ChinaMetabolic syndrome (MS) is a metabolic disease with multiple complications. Mulberry leaf extract (MLE) is rich in flavonoids and has great potential in alleviating glucose and lipid metabolism disorders. This study evaluated the effect and mechanism of MLE on the alleviation of MS. The components of the MLE were analyzed, and then the regulation of lipid metabolism by MLE in vitro and in vivo was determined. In a hepatocyte model of oleic acid-induced lipid accumulation, it was found that MLE alleviated lipid accumulation and decreased the expression of genes involved in lipogenesis. Furthermore, MLE improved obesity, insulin resistance, plasma lipid profile, and liver function in MS mice after a 15-week intervention. MLE decreased the expression of SREBP1, ACC, and FAS through the AMPK signaling pathway to inhibit lipid synthesis and increase the level of CPT1A to promote lipid decomposition to achieve its hypolipidemic effect. Meanwhile, MLE was also shown to affect the composition of the gut microbiota and the production of short-chain fatty acids, which contributed to the alleviation of lipid accumulation. Our results suggest that MLE can improve MS by improving lipid metabolism through multiple mechanisms and can be developed into dietary supplements for the improvement of MS.https://www.mdpi.com/1420-3049/27/16/5111metabolic syndromemulberry leaf extractflavonoidslipid metabolismgut microbiota |
| spellingShingle | Liangyu He Yan Xing Xinxiu Ren Mengjiao Zheng Shiqiang Yu Yinbo Wang Zhilong Xiu Yuesheng Dong Mulberry Leaf Extract Improves Metabolic Syndrome by Alleviating Lipid Accumulation In Vitro and In Vivo Molecules metabolic syndrome mulberry leaf extract flavonoids lipid metabolism gut microbiota |
| title | Mulberry Leaf Extract Improves Metabolic Syndrome by Alleviating Lipid Accumulation In Vitro and In Vivo |
| title_full | Mulberry Leaf Extract Improves Metabolic Syndrome by Alleviating Lipid Accumulation In Vitro and In Vivo |
| title_fullStr | Mulberry Leaf Extract Improves Metabolic Syndrome by Alleviating Lipid Accumulation In Vitro and In Vivo |
| title_full_unstemmed | Mulberry Leaf Extract Improves Metabolic Syndrome by Alleviating Lipid Accumulation In Vitro and In Vivo |
| title_short | Mulberry Leaf Extract Improves Metabolic Syndrome by Alleviating Lipid Accumulation In Vitro and In Vivo |
| title_sort | mulberry leaf extract improves metabolic syndrome by alleviating lipid accumulation in vitro and in vivo |
| topic | metabolic syndrome mulberry leaf extract flavonoids lipid metabolism gut microbiota |
| url | https://www.mdpi.com/1420-3049/27/16/5111 |
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