Pichinde virus induces microvascular endothelial cell permeability through the production of nitric oxide
<p>Abstract</p> <p>This report is the first to demonstrate infection of human endothelial cells by Pichinde virus (PIC). PIC infection induces an upregulation of the inducible nitric oxide synthase gene; as well as an increase in detectable nitric oxide (NO). PIC induces an increas...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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BMC
2009-10-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/6/1/162 |
| _version_ | 1818147626214752256 |
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| author | Voss Thomas G Brocato Rebecca L |
| author_facet | Voss Thomas G Brocato Rebecca L |
| author_sort | Voss Thomas G |
| collection | DOAJ |
| description | <p>Abstract</p> <p>This report is the first to demonstrate infection of human endothelial cells by Pichinde virus (PIC). PIC infection induces an upregulation of the inducible nitric oxide synthase gene; as well as an increase in detectable nitric oxide (NO). PIC induces an increase in permeability in endothelial cell monolayers which can be abrogated at all measured timepoints with the addition of a nitric oxide synthase inhibitor, indicating a role for NO in the alteration of endothelial barrier function. Because NO has shown antiviral activity against some viruses, viral titer was measured after addition of the NO synthase inhibitor and found to have no effect in altering virus load in infected EC. The NO synthase inhibition also has no effect on levels of activated caspases induced by PIC infection. Taken together, these data indicate NO production induced by Pichinde virus infection has a pathogenic effect on endothelial cell monolayer permeability.</p> |
| first_indexed | 2024-12-11T12:38:14Z |
| format | Article |
| id | doaj.art-6769807cff9b40d7b7fe9385b5c0d27a |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-11T12:38:14Z |
| publishDate | 2009-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-6769807cff9b40d7b7fe9385b5c0d27a2022-12-22T01:07:03ZengBMCVirology Journal1743-422X2009-10-016116210.1186/1743-422X-6-162Pichinde virus induces microvascular endothelial cell permeability through the production of nitric oxideVoss Thomas GBrocato Rebecca L<p>Abstract</p> <p>This report is the first to demonstrate infection of human endothelial cells by Pichinde virus (PIC). PIC infection induces an upregulation of the inducible nitric oxide synthase gene; as well as an increase in detectable nitric oxide (NO). PIC induces an increase in permeability in endothelial cell monolayers which can be abrogated at all measured timepoints with the addition of a nitric oxide synthase inhibitor, indicating a role for NO in the alteration of endothelial barrier function. Because NO has shown antiviral activity against some viruses, viral titer was measured after addition of the NO synthase inhibitor and found to have no effect in altering virus load in infected EC. The NO synthase inhibition also has no effect on levels of activated caspases induced by PIC infection. Taken together, these data indicate NO production induced by Pichinde virus infection has a pathogenic effect on endothelial cell monolayer permeability.</p>http://www.virologyj.com/content/6/1/162 |
| spellingShingle | Voss Thomas G Brocato Rebecca L Pichinde virus induces microvascular endothelial cell permeability through the production of nitric oxide Virology Journal |
| title | Pichinde virus induces microvascular endothelial cell permeability through the production of nitric oxide |
| title_full | Pichinde virus induces microvascular endothelial cell permeability through the production of nitric oxide |
| title_fullStr | Pichinde virus induces microvascular endothelial cell permeability through the production of nitric oxide |
| title_full_unstemmed | Pichinde virus induces microvascular endothelial cell permeability through the production of nitric oxide |
| title_short | Pichinde virus induces microvascular endothelial cell permeability through the production of nitric oxide |
| title_sort | pichinde virus induces microvascular endothelial cell permeability through the production of nitric oxide |
| url | http://www.virologyj.com/content/6/1/162 |
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