Genome-Based Analysis of a Sequence Type 1049 Hypervirulent Klebsiella pneumoniae Causing Bacteremic Neck Abscess
Hypervirulent Klebsiella pneumoniae (hvKP) has raised grave concerns in recent years and can cause severe infections with diverse anatomic locations including liver abscess, meningitis, and endophthalmitis. However, there is limited data about neck abscess caused by hvKP. A K. pneumoniae strain Kp_w...
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.617651/full |
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| author | Peng Lan Peng Lan Peng Lan Dongdong Zhao Jiong Gu Qiucheng Shi Rushuang Yan Rushuang Yan Yan Jiang Jiancang Zhou Yunsong Yu Yunsong Yu |
| author_facet | Peng Lan Peng Lan Peng Lan Dongdong Zhao Jiong Gu Qiucheng Shi Rushuang Yan Rushuang Yan Yan Jiang Jiancang Zhou Yunsong Yu Yunsong Yu |
| author_sort | Peng Lan |
| collection | DOAJ |
| description | Hypervirulent Klebsiella pneumoniae (hvKP) has raised grave concerns in recent years and can cause severe infections with diverse anatomic locations including liver abscess, meningitis, and endophthalmitis. However, there is limited data about neck abscess caused by hvKP. A K. pneumoniae strain Kp_whw was isolated from neck abscess. We characterized the genetic background, virulence determinates of the strain by genomic analysis and dertermined the virulence level by serum resistance assay. Kp_whw belonged to sequence type (ST) 1049 K locus (KL) 5. Kp_whw showed hypermucoviscosity phenotype and was resistant to ampicillin but susceptible to the majority of the other antimicrobial agents. A pLVPK-like virulence plasmid and a chromosomal ICEKp5-like mobile genetic element were carried by Kp_whw, resulting in the risk of dissemination of hypervirulence. The strain exhibited relative higher level of core genome allelic diversity than accessory genome profile, in comparison to hvKP of K1/K2 serotype. Kp_whw was finally demonstrated as virulent as the ST23 K1 serotype hvKP strain NTUH-K2044 in vitro. In conclusion, this work elaborates the genetic background of a clinical hvKP strain with an uncommon ST, reinforcing our understanding of virulence mechanisms of hvKP. |
| first_indexed | 2024-12-24T05:13:15Z |
| format | Article |
| id | doaj.art-676c7f445a6246989fe4ddf867a8aacc |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-24T05:13:15Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-676c7f445a6246989fe4ddf867a8aacc2022-12-21T17:13:38ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-01-011110.3389/fmicb.2020.617651617651Genome-Based Analysis of a Sequence Type 1049 Hypervirulent Klebsiella pneumoniae Causing Bacteremic Neck AbscessPeng Lan0Peng Lan1Peng Lan2Dongdong Zhao3Jiong Gu4Qiucheng Shi5Rushuang Yan6Rushuang Yan7Yan Jiang8Jiancang Zhou9Yunsong Yu10Yunsong Yu11Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaKey Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, ChinaDepartment of Critical Care Medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Infectious Diseases, The First Hospital of Jiaxing, Zhejiang, ChinaKey Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, ChinaKey Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, ChinaDepartment of Critical Care Medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaKey Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, ChinaDepartment of Critical Care Medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaDepartment of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaKey Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou, ChinaHypervirulent Klebsiella pneumoniae (hvKP) has raised grave concerns in recent years and can cause severe infections with diverse anatomic locations including liver abscess, meningitis, and endophthalmitis. However, there is limited data about neck abscess caused by hvKP. A K. pneumoniae strain Kp_whw was isolated from neck abscess. We characterized the genetic background, virulence determinates of the strain by genomic analysis and dertermined the virulence level by serum resistance assay. Kp_whw belonged to sequence type (ST) 1049 K locus (KL) 5. Kp_whw showed hypermucoviscosity phenotype and was resistant to ampicillin but susceptible to the majority of the other antimicrobial agents. A pLVPK-like virulence plasmid and a chromosomal ICEKp5-like mobile genetic element were carried by Kp_whw, resulting in the risk of dissemination of hypervirulence. The strain exhibited relative higher level of core genome allelic diversity than accessory genome profile, in comparison to hvKP of K1/K2 serotype. Kp_whw was finally demonstrated as virulent as the ST23 K1 serotype hvKP strain NTUH-K2044 in vitro. In conclusion, this work elaborates the genetic background of a clinical hvKP strain with an uncommon ST, reinforcing our understanding of virulence mechanisms of hvKP.https://www.frontiersin.org/articles/10.3389/fmicb.2020.617651/fullhypervirulentKlebsiella pneumoniaeneck abscessST1049comparative genomic analysis |
| spellingShingle | Peng Lan Peng Lan Peng Lan Dongdong Zhao Jiong Gu Qiucheng Shi Rushuang Yan Rushuang Yan Yan Jiang Jiancang Zhou Yunsong Yu Yunsong Yu Genome-Based Analysis of a Sequence Type 1049 Hypervirulent Klebsiella pneumoniae Causing Bacteremic Neck Abscess Frontiers in Microbiology hypervirulent Klebsiella pneumoniae neck abscess ST1049 comparative genomic analysis |
| title | Genome-Based Analysis of a Sequence Type 1049 Hypervirulent Klebsiella pneumoniae Causing Bacteremic Neck Abscess |
| title_full | Genome-Based Analysis of a Sequence Type 1049 Hypervirulent Klebsiella pneumoniae Causing Bacteremic Neck Abscess |
| title_fullStr | Genome-Based Analysis of a Sequence Type 1049 Hypervirulent Klebsiella pneumoniae Causing Bacteremic Neck Abscess |
| title_full_unstemmed | Genome-Based Analysis of a Sequence Type 1049 Hypervirulent Klebsiella pneumoniae Causing Bacteremic Neck Abscess |
| title_short | Genome-Based Analysis of a Sequence Type 1049 Hypervirulent Klebsiella pneumoniae Causing Bacteremic Neck Abscess |
| title_sort | genome based analysis of a sequence type 1049 hypervirulent klebsiella pneumoniae causing bacteremic neck abscess |
| topic | hypervirulent Klebsiella pneumoniae neck abscess ST1049 comparative genomic analysis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.617651/full |
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