Quantitative profiling of Drosophila melanogaster Dscam1 isoforms reveals no changes in splicing after bacterial exposure.
The hypervariable Dscam1 (Down syndrome cell adhesion molecule 1) gene can produce thousands of different ectodomain isoforms via mutually exclusive alternative splicing. Dscam1 appears to be involved in the immune response of some insects and crustaceans. It has been proposed that the diverse isofo...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4195611?pdf=render |
| _version_ | 1819027084860719104 |
|---|---|
| author | Sophie A O Armitage Wei Sun Xintian You Joachim Kurtz Dietmar Schmucker Wei Chen |
| author_facet | Sophie A O Armitage Wei Sun Xintian You Joachim Kurtz Dietmar Schmucker Wei Chen |
| author_sort | Sophie A O Armitage |
| collection | DOAJ |
| description | The hypervariable Dscam1 (Down syndrome cell adhesion molecule 1) gene can produce thousands of different ectodomain isoforms via mutually exclusive alternative splicing. Dscam1 appears to be involved in the immune response of some insects and crustaceans. It has been proposed that the diverse isoforms may be involved in the recognition of, or the defence against, diverse parasite epitopes, although evidence to support this is sparse. A prediction that can be generated from this hypothesis is that the gene expression of specific exons and/or isoforms is influenced by exposure to an immune elicitor. To test this hypothesis, we for the first time, use a long read RNA sequencing method to directly investigate the Dscam1 splicing pattern after exposing adult Drosophila melanogaster and a S2 cell line to live Escherichia coli. After bacterial exposure both models showed increased expression of immune-related genes, indicating that the immune system had been activated. However there were no changes in total Dscam1 mRNA expression. RNA sequencing further showed that there were no significant changes in individual exon expression and no changes in isoform splicing patterns in response to bacterial exposure. Therefore our studies do not support a change of D. melanogaster Dscam1 isoform diversity in response to live E. coli. Nevertheless, in future this approach could be used to identify potentially immune-related Dscam1 splicing regulation in other host species or in response to other pathogens. |
| first_indexed | 2024-12-21T05:36:51Z |
| format | Article |
| id | doaj.art-676e3403b28548f2ba46b1f7b5735bfb |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T05:36:51Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-676e3403b28548f2ba46b1f7b5735bfb2022-12-21T19:14:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e10866010.1371/journal.pone.0108660Quantitative profiling of Drosophila melanogaster Dscam1 isoforms reveals no changes in splicing after bacterial exposure.Sophie A O ArmitageWei SunXintian YouJoachim KurtzDietmar SchmuckerWei ChenThe hypervariable Dscam1 (Down syndrome cell adhesion molecule 1) gene can produce thousands of different ectodomain isoforms via mutually exclusive alternative splicing. Dscam1 appears to be involved in the immune response of some insects and crustaceans. It has been proposed that the diverse isoforms may be involved in the recognition of, or the defence against, diverse parasite epitopes, although evidence to support this is sparse. A prediction that can be generated from this hypothesis is that the gene expression of specific exons and/or isoforms is influenced by exposure to an immune elicitor. To test this hypothesis, we for the first time, use a long read RNA sequencing method to directly investigate the Dscam1 splicing pattern after exposing adult Drosophila melanogaster and a S2 cell line to live Escherichia coli. After bacterial exposure both models showed increased expression of immune-related genes, indicating that the immune system had been activated. However there were no changes in total Dscam1 mRNA expression. RNA sequencing further showed that there were no significant changes in individual exon expression and no changes in isoform splicing patterns in response to bacterial exposure. Therefore our studies do not support a change of D. melanogaster Dscam1 isoform diversity in response to live E. coli. Nevertheless, in future this approach could be used to identify potentially immune-related Dscam1 splicing regulation in other host species or in response to other pathogens.http://europepmc.org/articles/PMC4195611?pdf=render |
| spellingShingle | Sophie A O Armitage Wei Sun Xintian You Joachim Kurtz Dietmar Schmucker Wei Chen Quantitative profiling of Drosophila melanogaster Dscam1 isoforms reveals no changes in splicing after bacterial exposure. PLoS ONE |
| title | Quantitative profiling of Drosophila melanogaster Dscam1 isoforms reveals no changes in splicing after bacterial exposure. |
| title_full | Quantitative profiling of Drosophila melanogaster Dscam1 isoforms reveals no changes in splicing after bacterial exposure. |
| title_fullStr | Quantitative profiling of Drosophila melanogaster Dscam1 isoforms reveals no changes in splicing after bacterial exposure. |
| title_full_unstemmed | Quantitative profiling of Drosophila melanogaster Dscam1 isoforms reveals no changes in splicing after bacterial exposure. |
| title_short | Quantitative profiling of Drosophila melanogaster Dscam1 isoforms reveals no changes in splicing after bacterial exposure. |
| title_sort | quantitative profiling of drosophila melanogaster dscam1 isoforms reveals no changes in splicing after bacterial exposure |
| url | http://europepmc.org/articles/PMC4195611?pdf=render |
| work_keys_str_mv | AT sophieaoarmitage quantitativeprofilingofdrosophilamelanogasterdscam1isoformsrevealsnochangesinsplicingafterbacterialexposure AT weisun quantitativeprofilingofdrosophilamelanogasterdscam1isoformsrevealsnochangesinsplicingafterbacterialexposure AT xintianyou quantitativeprofilingofdrosophilamelanogasterdscam1isoformsrevealsnochangesinsplicingafterbacterialexposure AT joachimkurtz quantitativeprofilingofdrosophilamelanogasterdscam1isoformsrevealsnochangesinsplicingafterbacterialexposure AT dietmarschmucker quantitativeprofilingofdrosophilamelanogasterdscam1isoformsrevealsnochangesinsplicingafterbacterialexposure AT weichen quantitativeprofilingofdrosophilamelanogasterdscam1isoformsrevealsnochangesinsplicingafterbacterialexposure |