Prognostic significance of inflammatory biomarkers in hepatocellular carcinoma following hepatic resection
Background Cancer‐related inflammation has been correlated with cancer prognosis. This study evaluated inflammatory biomarkers, including neutrophil‐to‐lymphocyte ratio (NLR), platelet‐to‐lymphocyte ratio (PLR) and lymphocyte‐to‐monocyte ratio (LMR), programmed death ligand (PD‐L) 1 expression, and...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2019-08-01
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| Series: | BJS Open |
| Online Access: | https://doi.org/10.1002/bjs5.50170 |
| _version_ | 1818944286816731136 |
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| author | S. Itoh K. Yugawa M. Shimokawa S. Yoshiya Y. Mano K. Takeishi T. Toshima Y. Maehara M. Mori T. Yoshizumi |
| author_facet | S. Itoh K. Yugawa M. Shimokawa S. Yoshiya Y. Mano K. Takeishi T. Toshima Y. Maehara M. Mori T. Yoshizumi |
| author_sort | S. Itoh |
| collection | DOAJ |
| description | Background Cancer‐related inflammation has been correlated with cancer prognosis. This study evaluated inflammatory biomarkers, including neutrophil‐to‐lymphocyte ratio (NLR), platelet‐to‐lymphocyte ratio (PLR) and lymphocyte‐to‐monocyte ratio (LMR), programmed death ligand (PD‐L) 1 expression, and tumour microenvironment in relation to prognosis and clinicopathological features of patients with hepatocellular carcinoma (HCC) undergoing curative hepatic resection. Methods Patients who had liver resection for HCC in 2000–2011 were analysed. Univariable and multivariable analyses were conducted for overall (OS) and recurrence‐free (RFS) survival. Immunohistochemical analyses of PD‐L1, CD8 and CD68 expression were performed. HCC cell lines were evaluated for PD‐L1 expression. A subgroup analysis was conducted to determine patient features, survival and the tumour microenvironment. Results were validated in a cohort of patients with HCC treated surgically in 2012–2016. Results Some 281 patients who underwent hepatic resection for HCC were included. Multivariable analysis showed that low LMR was an independent prognostic factor of OS (hazard ratio (HR) 1·59, 95 per cent c.i. 1·00 to 2·41; P = 0·045) and RFS (HR 1·47, 1·05 to 2·04; P = 0·022) after resection. Low preoperative LMR values were correlated with higher α‐fetoprotein values (P < 0·001), larger tumour size (P < 0·001), and high rates of poor differentiation (P = 0·035) and liver cirrhosis (P = 0·008). LMR was significantly lower in PD‐L1‐positive patients than in those with PD‐L1 negativity (P < 0·001). Results were confirmed in the validation cohort. PD‐L1 expression was upregulated in HCC cell lines treated with interferon‐γ and co‐cultured with THP‐1 monocyte cells. Conclusion LMR is an independent predictor of survival after hepatic resection in patients with HCC. Modulation of the immune checkpoint pathway in the tumour microenvironment is associated with a low LMR. |
| first_indexed | 2024-12-20T07:40:49Z |
| format | Article |
| id | doaj.art-67707abe3eed4792a5e114267edf5528 |
| institution | Directory Open Access Journal |
| issn | 2474-9842 |
| language | English |
| last_indexed | 2024-12-20T07:40:49Z |
| publishDate | 2019-08-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | BJS Open |
| spelling | doaj.art-67707abe3eed4792a5e114267edf55282022-12-21T19:48:08ZengOxford University PressBJS Open2474-98422019-08-013450050810.1002/bjs5.50170Prognostic significance of inflammatory biomarkers in hepatocellular carcinoma following hepatic resectionS. Itoh0K. Yugawa1M. Shimokawa2S. Yoshiya3Y. Mano4K. Takeishi5T. Toshima6Y. Maehara7M. Mori8T. Yoshizumi9Department of Surgery and Science, Graduate School of Medical Sciences Kyushu University Fukuoka JapanDepartment of Surgery and Science, Graduate School of Medical Sciences Kyushu University Fukuoka JapanDepartment of Surgery and Science, Graduate School of Medical Sciences Kyushu University Fukuoka JapanDepartment of Surgery and Science, Graduate School of Medical Sciences Kyushu University Fukuoka JapanDepartment of Surgery and Science, Graduate School of Medical Sciences Kyushu University Fukuoka JapanDepartment of Surgery and Science, Graduate School of Medical Sciences Kyushu University Fukuoka JapanDepartment of Surgery and Science, Graduate School of Medical Sciences Kyushu University Fukuoka JapanDepartment of Surgery Kyushu Central Hospital of the Mutual Aid Association of Public School Teachers Fukuoka JapanDepartment of Surgery and Science, Graduate School of Medical Sciences Kyushu University Fukuoka JapanDepartment of Surgery and Science, Graduate School of Medical Sciences Kyushu University Fukuoka JapanBackground Cancer‐related inflammation has been correlated with cancer prognosis. This study evaluated inflammatory biomarkers, including neutrophil‐to‐lymphocyte ratio (NLR), platelet‐to‐lymphocyte ratio (PLR) and lymphocyte‐to‐monocyte ratio (LMR), programmed death ligand (PD‐L) 1 expression, and tumour microenvironment in relation to prognosis and clinicopathological features of patients with hepatocellular carcinoma (HCC) undergoing curative hepatic resection. Methods Patients who had liver resection for HCC in 2000–2011 were analysed. Univariable and multivariable analyses were conducted for overall (OS) and recurrence‐free (RFS) survival. Immunohistochemical analyses of PD‐L1, CD8 and CD68 expression were performed. HCC cell lines were evaluated for PD‐L1 expression. A subgroup analysis was conducted to determine patient features, survival and the tumour microenvironment. Results were validated in a cohort of patients with HCC treated surgically in 2012–2016. Results Some 281 patients who underwent hepatic resection for HCC were included. Multivariable analysis showed that low LMR was an independent prognostic factor of OS (hazard ratio (HR) 1·59, 95 per cent c.i. 1·00 to 2·41; P = 0·045) and RFS (HR 1·47, 1·05 to 2·04; P = 0·022) after resection. Low preoperative LMR values were correlated with higher α‐fetoprotein values (P < 0·001), larger tumour size (P < 0·001), and high rates of poor differentiation (P = 0·035) and liver cirrhosis (P = 0·008). LMR was significantly lower in PD‐L1‐positive patients than in those with PD‐L1 negativity (P < 0·001). Results were confirmed in the validation cohort. PD‐L1 expression was upregulated in HCC cell lines treated with interferon‐γ and co‐cultured with THP‐1 monocyte cells. Conclusion LMR is an independent predictor of survival after hepatic resection in patients with HCC. Modulation of the immune checkpoint pathway in the tumour microenvironment is associated with a low LMR.https://doi.org/10.1002/bjs5.50170 |
| spellingShingle | S. Itoh K. Yugawa M. Shimokawa S. Yoshiya Y. Mano K. Takeishi T. Toshima Y. Maehara M. Mori T. Yoshizumi Prognostic significance of inflammatory biomarkers in hepatocellular carcinoma following hepatic resection BJS Open |
| title | Prognostic significance of inflammatory biomarkers in hepatocellular carcinoma following hepatic resection |
| title_full | Prognostic significance of inflammatory biomarkers in hepatocellular carcinoma following hepatic resection |
| title_fullStr | Prognostic significance of inflammatory biomarkers in hepatocellular carcinoma following hepatic resection |
| title_full_unstemmed | Prognostic significance of inflammatory biomarkers in hepatocellular carcinoma following hepatic resection |
| title_short | Prognostic significance of inflammatory biomarkers in hepatocellular carcinoma following hepatic resection |
| title_sort | prognostic significance of inflammatory biomarkers in hepatocellular carcinoma following hepatic resection |
| url | https://doi.org/10.1002/bjs5.50170 |
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