Some Problems Arising during the Initiation of Somatic Embryogenesis in <i>Pinus sylvestris</i> L.
The use of biotechnological tools, in particular somatic embryogenesis (SE) for mass propagation of conifers, is relevant since this method allows to quickly replicate plant material with desired features. However, there are still a number of difficulties in obtaining an embryogenic cell culture for...
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MDPI AG
2022-11-01
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| Series: | Environmental Sciences Proceedings |
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| Online Access: | https://www.mdpi.com/2673-4931/22/1/48 |
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| author | Roman V. Ignatenko Olga V. Chirva Maria A. Ershova Natalia A. Galibina |
| author_facet | Roman V. Ignatenko Olga V. Chirva Maria A. Ershova Natalia A. Galibina |
| author_sort | Roman V. Ignatenko |
| collection | DOAJ |
| description | The use of biotechnological tools, in particular somatic embryogenesis (SE) for mass propagation of conifers, is relevant since this method allows to quickly replicate plant material with desired features. However, there are still a number of difficulties in obtaining an embryogenic cell culture for <i>Pinus sylvestris</i>. One of the important and unsolved problems is the search for SE-competent genotypes. We cultured 674 megagametophytes from 22 donor plants (16 genotypes) in vitro during the 2021 summer period. As a result of the experiment, callus formation was not recorded for the studied genotypes; however, 9.4 ± 1.0% of the explants formed plants. In addition to the genotype effect, unsuitable nutrient medium or late developmental stages of zygotic embryos could be the reasons for the lack of callus induction. To solve these problems, a number of studies were carried out: (1) the effect of the nutrient medium composition and density (MS, MSG, ½LV, DCR) on the callus initiation from mature seeds was analyzed, (2) the effect of various growth regulators concentrations on the initiation of callus formation was studied, (3) the analysis of the reproductive competence of donor plants was performed by the method of vegetative buds cultivation. As a result, several genotypes were found to have the ability for embryogenic callus formation, and the conditions for explants cultivation were selected. |
| first_indexed | 2024-03-11T06:33:55Z |
| format | Article |
| id | doaj.art-6775d501b4b64f7f9d0b9fae7fe3270e |
| institution | Directory Open Access Journal |
| issn | 2673-4931 |
| language | English |
| last_indexed | 2024-03-11T06:33:55Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Environmental Sciences Proceedings |
| spelling | doaj.art-6775d501b4b64f7f9d0b9fae7fe3270e2023-11-17T10:59:52ZengMDPI AGEnvironmental Sciences Proceedings2673-49312022-11-012214810.3390/IECF2022-13364Some Problems Arising during the Initiation of Somatic Embryogenesis in <i>Pinus sylvestris</i> L.Roman V. Ignatenko0Olga V. Chirva1Maria A. Ershova2Natalia A. Galibina3Department of Multidisciplinary Scientific Research, Karelian Research Centre, Russian Academy of Sciences, 11 Pushkinskaya St., 185910 Petrozavodsk, RussiaDepartment of Multidisciplinary Scientific Research, Karelian Research Centre, Russian Academy of Sciences, 11 Pushkinskaya St., 185910 Petrozavodsk, RussiaDepartment of Multidisciplinary Scientific Research, Karelian Research Centre, Russian Academy of Sciences, 11 Pushkinskaya St., 185910 Petrozavodsk, RussiaForest Research Institute, Karelian Research Centre, Russian Academy of Sciences, 11 Pushkinskaya St., 185910 Petrozavodsk, RussiaThe use of biotechnological tools, in particular somatic embryogenesis (SE) for mass propagation of conifers, is relevant since this method allows to quickly replicate plant material with desired features. However, there are still a number of difficulties in obtaining an embryogenic cell culture for <i>Pinus sylvestris</i>. One of the important and unsolved problems is the search for SE-competent genotypes. We cultured 674 megagametophytes from 22 donor plants (16 genotypes) in vitro during the 2021 summer period. As a result of the experiment, callus formation was not recorded for the studied genotypes; however, 9.4 ± 1.0% of the explants formed plants. In addition to the genotype effect, unsuitable nutrient medium or late developmental stages of zygotic embryos could be the reasons for the lack of callus induction. To solve these problems, a number of studies were carried out: (1) the effect of the nutrient medium composition and density (MS, MSG, ½LV, DCR) on the callus initiation from mature seeds was analyzed, (2) the effect of various growth regulators concentrations on the initiation of callus formation was studied, (3) the analysis of the reproductive competence of donor plants was performed by the method of vegetative buds cultivation. As a result, several genotypes were found to have the ability for embryogenic callus formation, and the conditions for explants cultivation were selected.https://www.mdpi.com/2673-4931/22/1/48somatic embryogenesisScots pinemedium compositionmature seedsvegetative budsKarelia |
| spellingShingle | Roman V. Ignatenko Olga V. Chirva Maria A. Ershova Natalia A. Galibina Some Problems Arising during the Initiation of Somatic Embryogenesis in <i>Pinus sylvestris</i> L. Environmental Sciences Proceedings somatic embryogenesis Scots pine medium composition mature seeds vegetative buds Karelia |
| title | Some Problems Arising during the Initiation of Somatic Embryogenesis in <i>Pinus sylvestris</i> L. |
| title_full | Some Problems Arising during the Initiation of Somatic Embryogenesis in <i>Pinus sylvestris</i> L. |
| title_fullStr | Some Problems Arising during the Initiation of Somatic Embryogenesis in <i>Pinus sylvestris</i> L. |
| title_full_unstemmed | Some Problems Arising during the Initiation of Somatic Embryogenesis in <i>Pinus sylvestris</i> L. |
| title_short | Some Problems Arising during the Initiation of Somatic Embryogenesis in <i>Pinus sylvestris</i> L. |
| title_sort | some problems arising during the initiation of somatic embryogenesis in i pinus sylvestris i l |
| topic | somatic embryogenesis Scots pine medium composition mature seeds vegetative buds Karelia |
| url | https://www.mdpi.com/2673-4931/22/1/48 |
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