Actinorhizal Signaling Molecules: Frankia Root Hair Deforming Factor Shares Properties With NIN Inducing Factor
Actinorhizal plants are able to establish a symbiotic relationship with Frankia bacteria leading to the formation of root nodules. The symbiotic interaction starts with the exchange of symbiotic signals in the soil between the plant and the bacteria. This molecular dialog involves signaling molecule...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2018.01494/full |
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| author | Maimouna Cissoko Maimouna Cissoko Maimouna Cissoko Maimouna Cissoko Valérie Hocher Hassen Gherbi Djamel Gully Alyssa Carré-Mlouka Alyssa Carré-Mlouka Seyni Sane Sarah Pignoly Sarah Pignoly Sarah Pignoly Antony Champion Antony Champion Antony Champion Mariama Ngom Mariama Ngom Petar Pujic Pascale Fournier Maher Gtari Erik Swanson Céline Pesce Louis S. Tisa Mame Oureye Sy Sergio Svistoonoff Sergio Svistoonoff Sergio Svistoonoff |
| author_facet | Maimouna Cissoko Maimouna Cissoko Maimouna Cissoko Maimouna Cissoko Valérie Hocher Hassen Gherbi Djamel Gully Alyssa Carré-Mlouka Alyssa Carré-Mlouka Seyni Sane Sarah Pignoly Sarah Pignoly Sarah Pignoly Antony Champion Antony Champion Antony Champion Mariama Ngom Mariama Ngom Petar Pujic Pascale Fournier Maher Gtari Erik Swanson Céline Pesce Louis S. Tisa Mame Oureye Sy Sergio Svistoonoff Sergio Svistoonoff Sergio Svistoonoff |
| author_sort | Maimouna Cissoko |
| collection | DOAJ |
| description | Actinorhizal plants are able to establish a symbiotic relationship with Frankia bacteria leading to the formation of root nodules. The symbiotic interaction starts with the exchange of symbiotic signals in the soil between the plant and the bacteria. This molecular dialog involves signaling molecules that are responsible for the specific recognition of the plant host and its endosymbiont. Here we studied two factors potentially involved in signaling between Frankia casuarinae and its actinorhizal host Casuarina glauca: (1) the Root Hair Deforming Factor (CgRHDF) detected using a test based on the characteristic deformation of C. glauca root hairs inoculated with F. casuarinae and (2) a NIN activating factor (CgNINA) which is able to activate the expression of CgNIN, a symbiotic gene expressed during preinfection stages of root hair development. We showed that CgRHDF and CgNINA corresponded to small thermoresistant molecules. Both factors were also hydrophilic and resistant to a chitinase digestion indicating structural differences from rhizobial Nod factors (NFs) or mycorrhizal Myc-LCOs. We also investigated the presence of CgNINA and CgRHDF in 16 Frankia strains representative of Frankia diversity. High levels of root hair deformation (RHD) and activation of ProCgNIN were detected for Casuarina-infective strains from clade Ic and closely related strains from clade Ia unable to nodulate C. glauca. Lower levels were present for distantly related strains belonging to clade III. No CgRHDF or CgNINA could be detected for Frankia coriariae (Clade II) or for uninfective strains from clade IV. |
| first_indexed | 2024-12-12T11:34:21Z |
| format | Article |
| id | doaj.art-6775fa30a1f2456bb98cd9e705be5d86 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T11:34:21Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-6775fa30a1f2456bb98cd9e705be5d862022-12-22T00:25:42ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-10-01910.3389/fpls.2018.01494401402Actinorhizal Signaling Molecules: Frankia Root Hair Deforming Factor Shares Properties With NIN Inducing FactorMaimouna Cissoko0Maimouna Cissoko1Maimouna Cissoko2Maimouna Cissoko3Valérie Hocher4Hassen Gherbi5Djamel Gully6Alyssa Carré-Mlouka7Alyssa Carré-Mlouka8Seyni Sane9Sarah Pignoly10Sarah Pignoly11Sarah Pignoly12Antony Champion13Antony Champion14Antony Champion15Mariama Ngom16Mariama Ngom17Petar Pujic18Pascale Fournier19Maher Gtari20Erik Swanson21Céline Pesce22Louis S. Tisa23Mame Oureye Sy24Sergio Svistoonoff25Sergio Svistoonoff26Sergio Svistoonoff27Laboratoire Commun de Microbiologie, Institut de Recherche pour le Développement/Institut Sénégalais de Recherches Agricoles/Université Cheikh Anta Diop, Centre de Recherche de Bel Air, Dakar, SenegalLaboratoire Mixte International Adaptation des Plantes et Microorganismes Associés Aux Stress Environnementaux, Centre de Recherche de Bel Air, Dakar, SenegalLaboratoire Campus de Biotechnologies Végétales, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop, Dakar, SenegalLaboratoire des Symbioses Tropicales et Méditerranéennes, Institut de Recherche pour le Développement/INRA/CIRAD, Université Montpellier/SupAgro, Montpellier, FranceLaboratoire des Symbioses Tropicales et Méditerranéennes, Institut de Recherche pour le Développement/INRA/CIRAD, Université Montpellier/SupAgro, Montpellier, FranceLaboratoire des Symbioses Tropicales et Méditerranéennes, Institut de Recherche pour le Développement/INRA/CIRAD, Université Montpellier/SupAgro, Montpellier, FranceLaboratoire des Symbioses Tropicales et Méditerranéennes, Institut de Recherche pour le Développement/INRA/CIRAD, Université Montpellier/SupAgro, Montpellier, FranceLaboratoire des Symbioses Tropicales et Méditerranéennes, Institut de Recherche pour le Développement/INRA/CIRAD, Université Montpellier/SupAgro, Montpellier, FranceUMR 7245, Molécules de Communication et Adaptation des Microorganismes, Muséum National d’Histoire Naturelle, Centre National de la Recherche Scientifique, Sorbonne Universités, Paris, FranceLaboratoire de Botanique et de Biodiversité Végétale, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop, Dakar, SenegalLaboratoire Commun de Microbiologie, Institut de Recherche pour le Développement/Institut Sénégalais de Recherches Agricoles/Université Cheikh Anta Diop, Centre de Recherche de Bel Air, Dakar, SenegalLaboratoire Mixte International Adaptation des Plantes et Microorganismes Associés Aux Stress Environnementaux, Centre de Recherche de Bel Air, Dakar, SenegalLaboratoire des Symbioses Tropicales et Méditerranéennes, Institut de Recherche pour le Développement/INRA/CIRAD, Université Montpellier/SupAgro, Montpellier, FranceLaboratoire Commun de Microbiologie, Institut de Recherche pour le Développement/Institut Sénégalais de Recherches Agricoles/Université Cheikh Anta Diop, Centre de Recherche de Bel Air, Dakar, SenegalLaboratoire Mixte International Adaptation des Plantes et Microorganismes Associés Aux Stress Environnementaux, Centre de Recherche de Bel Air, Dakar, SenegalUMR Diversité Adaptation et Développement des Plantes (DIADE), Institut de Recherche pour le Développement, Montpellier, FranceLaboratoire Commun de Microbiologie, Institut de Recherche pour le Développement/Institut Sénégalais de Recherches Agricoles/Université Cheikh Anta Diop, Centre de Recherche de Bel Air, Dakar, SenegalLaboratoire Mixte International Adaptation des Plantes et Microorganismes Associés Aux Stress Environnementaux, Centre de Recherche de Bel Air, Dakar, SenegalEcologie Microbienne, UMR 5557 CNRS, Université Lyon 1, Villeurbanne, FranceEcologie Microbienne, UMR 5557 CNRS, Université Lyon 1, Villeurbanne, FranceInstitut National des Sciences Appliquées et de Technologie, Université Carthage, Tunis, Tunisia0Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, United States0Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, United States0Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, United StatesLaboratoire Campus de Biotechnologies Végétales, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop, Dakar, SenegalLaboratoire Commun de Microbiologie, Institut de Recherche pour le Développement/Institut Sénégalais de Recherches Agricoles/Université Cheikh Anta Diop, Centre de Recherche de Bel Air, Dakar, SenegalLaboratoire Mixte International Adaptation des Plantes et Microorganismes Associés Aux Stress Environnementaux, Centre de Recherche de Bel Air, Dakar, SenegalLaboratoire des Symbioses Tropicales et Méditerranéennes, Institut de Recherche pour le Développement/INRA/CIRAD, Université Montpellier/SupAgro, Montpellier, FranceActinorhizal plants are able to establish a symbiotic relationship with Frankia bacteria leading to the formation of root nodules. The symbiotic interaction starts with the exchange of symbiotic signals in the soil between the plant and the bacteria. This molecular dialog involves signaling molecules that are responsible for the specific recognition of the plant host and its endosymbiont. Here we studied two factors potentially involved in signaling between Frankia casuarinae and its actinorhizal host Casuarina glauca: (1) the Root Hair Deforming Factor (CgRHDF) detected using a test based on the characteristic deformation of C. glauca root hairs inoculated with F. casuarinae and (2) a NIN activating factor (CgNINA) which is able to activate the expression of CgNIN, a symbiotic gene expressed during preinfection stages of root hair development. We showed that CgRHDF and CgNINA corresponded to small thermoresistant molecules. Both factors were also hydrophilic and resistant to a chitinase digestion indicating structural differences from rhizobial Nod factors (NFs) or mycorrhizal Myc-LCOs. We also investigated the presence of CgNINA and CgRHDF in 16 Frankia strains representative of Frankia diversity. High levels of root hair deformation (RHD) and activation of ProCgNIN were detected for Casuarina-infective strains from clade Ic and closely related strains from clade Ia unable to nodulate C. glauca. Lower levels were present for distantly related strains belonging to clade III. No CgRHDF or CgNINA could be detected for Frankia coriariae (Clade II) or for uninfective strains from clade IV.https://www.frontiersin.org/article/10.3389/fpls.2018.01494/fullsymbiosesnodulation factorsnodule inceptionCasuarinaAlnusDiscaria |
| spellingShingle | Maimouna Cissoko Maimouna Cissoko Maimouna Cissoko Maimouna Cissoko Valérie Hocher Hassen Gherbi Djamel Gully Alyssa Carré-Mlouka Alyssa Carré-Mlouka Seyni Sane Sarah Pignoly Sarah Pignoly Sarah Pignoly Antony Champion Antony Champion Antony Champion Mariama Ngom Mariama Ngom Petar Pujic Pascale Fournier Maher Gtari Erik Swanson Céline Pesce Louis S. Tisa Mame Oureye Sy Sergio Svistoonoff Sergio Svistoonoff Sergio Svistoonoff Actinorhizal Signaling Molecules: Frankia Root Hair Deforming Factor Shares Properties With NIN Inducing Factor Frontiers in Plant Science symbioses nodulation factors nodule inception Casuarina Alnus Discaria |
| title | Actinorhizal Signaling Molecules: Frankia Root Hair Deforming Factor Shares Properties With NIN Inducing Factor |
| title_full | Actinorhizal Signaling Molecules: Frankia Root Hair Deforming Factor Shares Properties With NIN Inducing Factor |
| title_fullStr | Actinorhizal Signaling Molecules: Frankia Root Hair Deforming Factor Shares Properties With NIN Inducing Factor |
| title_full_unstemmed | Actinorhizal Signaling Molecules: Frankia Root Hair Deforming Factor Shares Properties With NIN Inducing Factor |
| title_short | Actinorhizal Signaling Molecules: Frankia Root Hair Deforming Factor Shares Properties With NIN Inducing Factor |
| title_sort | actinorhizal signaling molecules frankia root hair deforming factor shares properties with nin inducing factor |
| topic | symbioses nodulation factors nodule inception Casuarina Alnus Discaria |
| url | https://www.frontiersin.org/article/10.3389/fpls.2018.01494/full |
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