Relationship between sperm protamine deficiency and apoptosis in couples with unexplained repeated spontaneous abortions
Background: Etiology of more than half of Recurrent Spontaneous Abortion. The etiology of more than 50 percent of Recurrent Spontaneous Abortions (RSA) cases has been remained unexplained. It is supposed that RSA may have "paternal effect" due to supply 50% of embryonic genomic content by...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Shahid Sadoughi University of Medical Science, Yazd, Iran
2016-03-01
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| Series: | Iranian Journal of Reproductive Medicine |
| Online Access: | http://www.ssu.ac.ir/ijrm/index.php/ijrm/article/view/1856/962 |
| _version_ | 1819051767411769344 |
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| author | Ali Reza Talebi Farzaneh Fesahat Esmat Mangoli Jalal Ghasemzadeh Maryam Nayeri Fatemeh Sadeghian-Nodoshan |
| author_facet | Ali Reza Talebi Farzaneh Fesahat Esmat Mangoli Jalal Ghasemzadeh Maryam Nayeri Fatemeh Sadeghian-Nodoshan |
| author_sort | Ali Reza Talebi |
| collection | DOAJ |
| description | Background: Etiology of more than half of Recurrent Spontaneous Abortion. The etiology of more than 50 percent of Recurrent Spontaneous Abortions (RSA) cases has been remained unexplained. It is supposed that RSA may have "paternal effect" due to supply 50% of embryonic genomic content by male gamete.
Objective: The aim of present study was to evaluate the role of sperm apoptosis and protamine deficiency at same time in RSA cases.
Materials and Methods: Forty fertile (control) and 40 unfertile men with RSA (case) were enrolled in this case-control study. Semen analysis was performed in accordance with WHO criteria and sperm apoptosis and protamine deficiency were evaluated by cell apoptosis detection kit and chromomycin A3, respectively.
Results: Results showed significant different between normal morphology and total motility in two groups. Case group had higher percentage of spermatozoa with protamine deficiency and apoptosis compared to controls significantly.
Conclusion: Our results showed that in cases of RSA, in addition to abnormal sperm parameters, we have a high percentage of spermatozoa with protamine deficiency and apoptosis and these two anomalies may consider as important causes of idiopathic recurrent abortions. It should be advised that sperm chromatin and DNA examinations are useful tools in the process of RSA treatments. |
| first_indexed | 2024-12-21T12:09:10Z |
| format | Article |
| id | doaj.art-67776ea54f814fecae5ce1499ffdcd30 |
| institution | Directory Open Access Journal |
| issn | 1680-6433 2008-2177 |
| language | English |
| last_indexed | 2024-12-21T12:09:10Z |
| publishDate | 2016-03-01 |
| publisher | Shahid Sadoughi University of Medical Science, Yazd, Iran |
| record_format | Article |
| series | Iranian Journal of Reproductive Medicine |
| spelling | doaj.art-67776ea54f814fecae5ce1499ffdcd302022-12-21T19:04:37ZengShahid Sadoughi University of Medical Science, Yazd, IranIranian Journal of Reproductive Medicine1680-64332008-21772016-03-01143199204Relationship between sperm protamine deficiency and apoptosis in couples with unexplained repeated spontaneous abortionsAli Reza Talebi0Farzaneh Fesahat1Esmat Mangoli2Jalal Ghasemzadeh3Maryam Nayeri4Fatemeh Sadeghian-Nodoshan5Infertility Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IranInfertility Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IranInfertility Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IranInfertility Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Biology, Islamic Azad University of Ashkezar, Ashkezar, Iran.Stem Cell Biology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IranBackground: Etiology of more than half of Recurrent Spontaneous Abortion. The etiology of more than 50 percent of Recurrent Spontaneous Abortions (RSA) cases has been remained unexplained. It is supposed that RSA may have "paternal effect" due to supply 50% of embryonic genomic content by male gamete. Objective: The aim of present study was to evaluate the role of sperm apoptosis and protamine deficiency at same time in RSA cases. Materials and Methods: Forty fertile (control) and 40 unfertile men with RSA (case) were enrolled in this case-control study. Semen analysis was performed in accordance with WHO criteria and sperm apoptosis and protamine deficiency were evaluated by cell apoptosis detection kit and chromomycin A3, respectively. Results: Results showed significant different between normal morphology and total motility in two groups. Case group had higher percentage of spermatozoa with protamine deficiency and apoptosis compared to controls significantly. Conclusion: Our results showed that in cases of RSA, in addition to abnormal sperm parameters, we have a high percentage of spermatozoa with protamine deficiency and apoptosis and these two anomalies may consider as important causes of idiopathic recurrent abortions. It should be advised that sperm chromatin and DNA examinations are useful tools in the process of RSA treatments.http://www.ssu.ac.ir/ijrm/index.php/ijrm/article/view/1856/962 |
| spellingShingle | Ali Reza Talebi Farzaneh Fesahat Esmat Mangoli Jalal Ghasemzadeh Maryam Nayeri Fatemeh Sadeghian-Nodoshan Relationship between sperm protamine deficiency and apoptosis in couples with unexplained repeated spontaneous abortions Iranian Journal of Reproductive Medicine |
| title | Relationship between sperm protamine deficiency and apoptosis in couples with unexplained repeated spontaneous abortions |
| title_full | Relationship between sperm protamine deficiency and apoptosis in couples with unexplained repeated spontaneous abortions |
| title_fullStr | Relationship between sperm protamine deficiency and apoptosis in couples with unexplained repeated spontaneous abortions |
| title_full_unstemmed | Relationship between sperm protamine deficiency and apoptosis in couples with unexplained repeated spontaneous abortions |
| title_short | Relationship between sperm protamine deficiency and apoptosis in couples with unexplained repeated spontaneous abortions |
| title_sort | relationship between sperm protamine deficiency and apoptosis in couples with unexplained repeated spontaneous abortions |
| url | http://www.ssu.ac.ir/ijrm/index.php/ijrm/article/view/1856/962 |
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