Single cell analysis reveals satellite cell heterogeneity for proinflammatory chemokine expression
Background: The expression of proinflammatory signals at the site of muscle injury are essential for efficient tissue repair and their dysregulation can lead to inflammatory myopathies. Macrophages, neutrophils, and fibroadipogenic progenitor cells residing in the muscle are significant sources of p...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2023.1084068/full |
| _version_ | 1797859691702779904 |
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| author | Alexander B. Andre Alexander B. Andre Katherina P. Rees Katherina P. Rees Samantha O’Connor Samantha O’Connor Grant W. Severson Grant W. Severson Jason M. Newbern Jeanne Wilson-Rawls Christopher L. Plaisier Alan Rawls |
| author_facet | Alexander B. Andre Alexander B. Andre Katherina P. Rees Katherina P. Rees Samantha O’Connor Samantha O’Connor Grant W. Severson Grant W. Severson Jason M. Newbern Jeanne Wilson-Rawls Christopher L. Plaisier Alan Rawls |
| author_sort | Alexander B. Andre |
| collection | DOAJ |
| description | Background: The expression of proinflammatory signals at the site of muscle injury are essential for efficient tissue repair and their dysregulation can lead to inflammatory myopathies. Macrophages, neutrophils, and fibroadipogenic progenitor cells residing in the muscle are significant sources of proinflammatory cytokines and chemokines. However, the inducibility of the myogenic satellite cell population and their contribution to proinflammatory signaling is less understood.Methods: Mouse satellite cells were isolated and exposed to lipopolysaccharide (LPS) to mimic sterile skeletal muscle injury and changes in the expression of proinflammatory genes was examined by RT-qPCR and single cell RNA sequencing. Expression patterns were validated in skeletal muscle injured with cardiotoxin by RT-qPCR and immunofluorescence.Results: Satellite cells in culture were able to express Tnfa, Ccl2, and Il6, within 2 h of treatment with LPS. Single cell RNA-Seq revealed seven cell clusters representing the continuum from activation to differentiation. LPS treatment led to a heterogeneous pattern of induction of C-C and C-X-C chemokines (e.g., Ccl2, Ccl5, and Cxcl0) and cytokines (e.g., Tgfb1, Bmp2, Il18, and Il33) associated with innate immune cell recruitment and satellite cell proliferation. One cell cluster was enriched for expression of the antiviral interferon pathway genes under control conditions and LPS treatment. Activation of this pathway in satellite cells was also detectable at the site of cardiotoxin induced muscle injury.Conclusion: These data demonstrate that satellite cells respond to inflammatory signals and secrete chemokines and cytokines. Further, we identified a previously unrecognized subset of satellite cells that may act as sensors for muscle infection or injury using the antiviral interferon pathway. |
| first_indexed | 2024-04-09T21:34:45Z |
| format | Article |
| id | doaj.art-67782f314e6b445991619e95e0404d50 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-09T21:34:45Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-67782f314e6b445991619e95e0404d502023-03-27T04:42:38ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2023-03-011110.3389/fcell.2023.10840681084068Single cell analysis reveals satellite cell heterogeneity for proinflammatory chemokine expressionAlexander B. Andre0Alexander B. Andre1Katherina P. Rees2Katherina P. Rees3Samantha O’Connor4Samantha O’Connor5Grant W. Severson6Grant W. Severson7Jason M. Newbern8Jeanne Wilson-Rawls9Christopher L. Plaisier10Alan Rawls11School of Life Sciences, Arizona State University, Tempe, AZ, United StatesMolecular and Cellular Biology Graduate Program, Arizona State University, Tempe, AZ, United StatesSchool of Life Sciences, Arizona State University, Tempe, AZ, United StatesMolecular and Cellular Biology Graduate Program, Arizona State University, Tempe, AZ, United StatesSchool of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, United StatesBiomedical Engineering Graduate Program, Arizona State University, Tempe, AZ, United StatesSchool of Life Sciences, Arizona State University, Tempe, AZ, United StatesMolecular and Cellular Biology Graduate Program, Arizona State University, Tempe, AZ, United StatesSchool of Life Sciences, Arizona State University, Tempe, AZ, United StatesSchool of Life Sciences, Arizona State University, Tempe, AZ, United StatesSchool of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, United StatesSchool of Life Sciences, Arizona State University, Tempe, AZ, United StatesBackground: The expression of proinflammatory signals at the site of muscle injury are essential for efficient tissue repair and their dysregulation can lead to inflammatory myopathies. Macrophages, neutrophils, and fibroadipogenic progenitor cells residing in the muscle are significant sources of proinflammatory cytokines and chemokines. However, the inducibility of the myogenic satellite cell population and their contribution to proinflammatory signaling is less understood.Methods: Mouse satellite cells were isolated and exposed to lipopolysaccharide (LPS) to mimic sterile skeletal muscle injury and changes in the expression of proinflammatory genes was examined by RT-qPCR and single cell RNA sequencing. Expression patterns were validated in skeletal muscle injured with cardiotoxin by RT-qPCR and immunofluorescence.Results: Satellite cells in culture were able to express Tnfa, Ccl2, and Il6, within 2 h of treatment with LPS. Single cell RNA-Seq revealed seven cell clusters representing the continuum from activation to differentiation. LPS treatment led to a heterogeneous pattern of induction of C-C and C-X-C chemokines (e.g., Ccl2, Ccl5, and Cxcl0) and cytokines (e.g., Tgfb1, Bmp2, Il18, and Il33) associated with innate immune cell recruitment and satellite cell proliferation. One cell cluster was enriched for expression of the antiviral interferon pathway genes under control conditions and LPS treatment. Activation of this pathway in satellite cells was also detectable at the site of cardiotoxin induced muscle injury.Conclusion: These data demonstrate that satellite cells respond to inflammatory signals and secrete chemokines and cytokines. Further, we identified a previously unrecognized subset of satellite cells that may act as sensors for muscle infection or injury using the antiviral interferon pathway.https://www.frontiersin.org/articles/10.3389/fcell.2023.1084068/fullsatellite cellschemokinesscRNA-seqinterferoninflammationmuscle repair |
| spellingShingle | Alexander B. Andre Alexander B. Andre Katherina P. Rees Katherina P. Rees Samantha O’Connor Samantha O’Connor Grant W. Severson Grant W. Severson Jason M. Newbern Jeanne Wilson-Rawls Christopher L. Plaisier Alan Rawls Single cell analysis reveals satellite cell heterogeneity for proinflammatory chemokine expression Frontiers in Cell and Developmental Biology satellite cells chemokines scRNA-seq interferon inflammation muscle repair |
| title | Single cell analysis reveals satellite cell heterogeneity for proinflammatory chemokine expression |
| title_full | Single cell analysis reveals satellite cell heterogeneity for proinflammatory chemokine expression |
| title_fullStr | Single cell analysis reveals satellite cell heterogeneity for proinflammatory chemokine expression |
| title_full_unstemmed | Single cell analysis reveals satellite cell heterogeneity for proinflammatory chemokine expression |
| title_short | Single cell analysis reveals satellite cell heterogeneity for proinflammatory chemokine expression |
| title_sort | single cell analysis reveals satellite cell heterogeneity for proinflammatory chemokine expression |
| topic | satellite cells chemokines scRNA-seq interferon inflammation muscle repair |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2023.1084068/full |
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