A Novel Propidium Monoazide-Based PCR Assay Can Measure Viable Uropathogenic E. coli In Vitro and In Vivo

A Novel Propidium Monoazide-Based PCR Assay Can Measure Viable Uropathogenic E. coli In Vitro and In Vivo

BackgroundPolymerase chain reaction (PCR) is an important means by which to study the urine microbiome and is emerging as possible alternative to urine cultures to identify pathogens that cause urinary tract infection (UTI). However, PCR is limited by its inability to differentiate DNA originating f...

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Bibliographic Details
Main Authors: Albert S. Lee, Olivia K. Lamanna, Kenji Ishida, Elaise Hill, Andrew Nguyen, Michael H. Hsieh
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-02-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
propidium monoazide
viability
urine
microbiome
non-culturable bacteria
urobiome
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2022.794323/full

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