Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment
Currently tuberculosis is considered as a group of diseases united by one etiological factor. The pathogenesis of certain localizations of tuberculous inflammation, in particular peritoneum tuberculosis, hasn’t been sufficiently studied. The role of cytokine mechanisms in the development of the dise...
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| Format: | Article |
| Language: | Russian |
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Scientific Сentre for Family Health and Human Reproduction Problems
2021-11-01
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| Series: | Acta Biomedica Scientifica |
| Subjects: | |
| Online Access: | https://www.actabiomedica.ru/jour/article/view/3049 |
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| author | D. V. Plotkin T. I. Vinogradova M. N. Reshetnikov Yu. R. Zyuzya S. V. Okovityi M. V. Sinitsyn V. R. Gaitukaev G. V. Rodoman E. M. Bogorodskaya P. K. Yablonskiy |
| author_facet | D. V. Plotkin T. I. Vinogradova M. N. Reshetnikov Yu. R. Zyuzya S. V. Okovityi M. V. Sinitsyn V. R. Gaitukaev G. V. Rodoman E. M. Bogorodskaya P. K. Yablonskiy |
| author_sort | D. V. Plotkin |
| collection | DOAJ |
| description | Currently tuberculosis is considered as a group of diseases united by one etiological factor. The pathogenesis of certain localizations of tuberculous inflammation, in particular peritoneum tuberculosis, hasn’t been sufficiently studied. The role of cytokine mechanisms in the development of the disease and the elaboration of non-sterile immunity requires further experimental studies, in particular the creation of a reproducible model on laboratory animals.The aim: to study the effect of TNF-α on the development of tuberculosis of the serous coat of the abdominal cavity, as well as to evaluate the possibility of modeling tuberculous peritonitis in laboratory animals using infliximab.Materials and methods. The studies were conducted on 18 male rabbits, which were simulated peritoneal tuberculosis by intra-abdominal administration of a suspension of Mycobacterium tuberculosis. 10 rabbits of the experimental group were intravenously injected with an infliximab solution and an iron (III) hydroxide sucrose complex intraperitoneally a day before infection.Results. In the control group of animals, tuberculosis either didn’t develop, or in a third of cases it affected only the pulmonary parenchyma, while proliferative processes prevailed. On the contrary, in animals with inactivated TNF-α, in 100 % of observations, tuberculous peritonitis was detected with associated lung damage and the predominance of alterative caseous processes.Conclusion. The created model of tuberculous peritonitis shows the leading role of TNF-α in the activation of macrophages, as well as in attracting cells to the site of infection. This is the primary signal necessary for the formation and stability of granulomas since the neutralization of this cytokine leads to a loss of control over the infection and the destruction of the granuloma with the development of destructive tuberculosis in the serous coat of the abdominal cavity. |
| first_indexed | 2024-03-08T12:15:56Z |
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| id | doaj.art-677aa0645f874708a609b79c51904b5b |
| institution | Directory Open Access Journal |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| last_indexed | 2024-04-24T17:16:03Z |
| publishDate | 2021-11-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
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| series | Acta Biomedica Scientifica |
| spelling | doaj.art-677aa0645f874708a609b79c51904b5b2024-03-28T07:49:25ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962021-11-016518419510.29413/ABS.2021-6.5.182224Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experimentD. V. Plotkin0T. I. Vinogradova1M. N. Reshetnikov2Yu. R. Zyuzya3S. V. Okovityi4M. V. Sinitsyn5V. R. Gaitukaev6G. V. Rodoman7E. M. Bogorodskaya8P. K. Yablonskiy9Moscow Research and Clinical Center for TB Control, Moscow Healthcare Department; Pirogov Russian National Research Medical University of the Ministry of Healthcare of the Russian FederationSaint Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian FederationMoscow Research and Clinical Center for TB Control, Moscow Healthcare DepartmentMoscow Research and Clinical Center for TB Control, Moscow Healthcare DepartmentSaint Petersburg State Chemical Pharmaceutical University of the Ministry of Healthcare of the Russian FederationMoscow Research and Clinical Center for TB Control, Moscow Healthcare DepartmentOLLA-MED CompanyPirogov Russian National Research Medical University of the Ministry of Healthcare of the Russian FederationMoscow Research and Clinical Center for TB Control, Moscow Healthcare DepartmentSaint Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian FederationCurrently tuberculosis is considered as a group of diseases united by one etiological factor. The pathogenesis of certain localizations of tuberculous inflammation, in particular peritoneum tuberculosis, hasn’t been sufficiently studied. The role of cytokine mechanisms in the development of the disease and the elaboration of non-sterile immunity requires further experimental studies, in particular the creation of a reproducible model on laboratory animals.The aim: to study the effect of TNF-α on the development of tuberculosis of the serous coat of the abdominal cavity, as well as to evaluate the possibility of modeling tuberculous peritonitis in laboratory animals using infliximab.Materials and methods. The studies were conducted on 18 male rabbits, which were simulated peritoneal tuberculosis by intra-abdominal administration of a suspension of Mycobacterium tuberculosis. 10 rabbits of the experimental group were intravenously injected with an infliximab solution and an iron (III) hydroxide sucrose complex intraperitoneally a day before infection.Results. In the control group of animals, tuberculosis either didn’t develop, or in a third of cases it affected only the pulmonary parenchyma, while proliferative processes prevailed. On the contrary, in animals with inactivated TNF-α, in 100 % of observations, tuberculous peritonitis was detected with associated lung damage and the predominance of alterative caseous processes.Conclusion. The created model of tuberculous peritonitis shows the leading role of TNF-α in the activation of macrophages, as well as in attracting cells to the site of infection. This is the primary signal necessary for the formation and stability of granulomas since the neutralization of this cytokine leads to a loss of control over the infection and the destruction of the granuloma with the development of destructive tuberculosis in the serous coat of the abdominal cavity.https://www.actabiomedica.ru/jour/article/view/3049tuberculous peritonitisrabbittumor necrosis factormodel of tuberculous peritonitisinfliximab |
| spellingShingle | D. V. Plotkin T. I. Vinogradova M. N. Reshetnikov Yu. R. Zyuzya S. V. Okovityi M. V. Sinitsyn V. R. Gaitukaev G. V. Rodoman E. M. Bogorodskaya P. K. Yablonskiy Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment Acta Biomedica Scientifica tuberculous peritonitis rabbit tumor necrosis factor model of tuberculous peritonitis infliximab |
| title | Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment |
| title_full | Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment |
| title_fullStr | Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment |
| title_full_unstemmed | Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment |
| title_short | Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment |
| title_sort | pathogenetic role of tumor necrosis factor tnf α for the development of peritoneal tuberculosis in an experiment |
| topic | tuberculous peritonitis rabbit tumor necrosis factor model of tuberculous peritonitis infliximab |
| url | https://www.actabiomedica.ru/jour/article/view/3049 |
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