Mannose Receptor Ligands Regulate the Gene Expression of Toll-like Receptors in Chicken Monocytes
Pattern recognition receptors play an important role in distinguishing foreign organisms and triggering host defense responses. In this study, we present evidence for differential immune responses in chicken monocytes exposed to the mannose receptor (MR) ligands mannan and horseradish peroxidase (H...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Japan Poultry Science Association
2013-10-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/50/4/50_0120178/_pdf/-char/en |
| _version_ | 1797816181422292992 |
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| author | Li Yan Zandong Li |
| author_facet | Li Yan Zandong Li |
| author_sort | Li Yan |
| collection | DOAJ |
| description | Pattern recognition receptors play an important role in distinguishing foreign organisms and triggering host defense responses. In this study, we present evidence for differential immune responses in chicken monocytes exposed to the mannose receptor (MR) ligands mannan and horseradish peroxidase (HRP). In our study, mannan significantly up-regulated the expression of the MR gene at a high concentration of 200 μg/ml, but HRP did not. In stimulation experiments, neither mannan nor HRP induced nitric oxide (NO) production or stimulated cytokine (IL-1β, IL-6, and IL-10) gene expression. A concentration of 200 μg/ml mannan induced a significant increase in Toll-like receptor 2 (TLR2) gene expression. The gene expression of TLR1 and 3 was significantly up-regulated by different HRP concentrations. In contrast to TLR1 and 3, TLR4 exhibited a significant decrease in gene expression in a dose-dependent manner. These results may suggest that MR ligands affect the innate immune response of TLRs in chicken monocytes. |
| first_indexed | 2024-03-13T08:33:57Z |
| format | Article |
| id | doaj.art-677dc72ff71148f6b4bd31b31804fd7d |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:33:57Z |
| publishDate | 2013-10-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-677dc72ff71148f6b4bd31b31804fd7d2023-05-31T01:28:36ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862013-10-0150438839510.2141/jpsa.0120178jpsaMannose Receptor Ligands Regulate the Gene Expression of Toll-like Receptors in Chicken MonocytesLi Yan0Zandong Li1State Key Laboratories of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Science, China Agricultural University, ChinaState Key Laboratories of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Science, China Agricultural University, ChinaPattern recognition receptors play an important role in distinguishing foreign organisms and triggering host defense responses. In this study, we present evidence for differential immune responses in chicken monocytes exposed to the mannose receptor (MR) ligands mannan and horseradish peroxidase (HRP). In our study, mannan significantly up-regulated the expression of the MR gene at a high concentration of 200 μg/ml, but HRP did not. In stimulation experiments, neither mannan nor HRP induced nitric oxide (NO) production or stimulated cytokine (IL-1β, IL-6, and IL-10) gene expression. A concentration of 200 μg/ml mannan induced a significant increase in Toll-like receptor 2 (TLR2) gene expression. The gene expression of TLR1 and 3 was significantly up-regulated by different HRP concentrations. In contrast to TLR1 and 3, TLR4 exhibited a significant decrease in gene expression in a dose-dependent manner. These results may suggest that MR ligands affect the innate immune response of TLRs in chicken monocytes.https://www.jstage.jst.go.jp/article/jpsa/50/4/50_0120178/_pdf/-char/enchickenmannose receptormonocytestoll-like receptor |
| spellingShingle | Li Yan Zandong Li Mannose Receptor Ligands Regulate the Gene Expression of Toll-like Receptors in Chicken Monocytes The Journal of Poultry Science chicken mannose receptor monocytes toll-like receptor |
| title | Mannose Receptor Ligands Regulate the Gene Expression of Toll-like Receptors in Chicken Monocytes |
| title_full | Mannose Receptor Ligands Regulate the Gene Expression of Toll-like Receptors in Chicken Monocytes |
| title_fullStr | Mannose Receptor Ligands Regulate the Gene Expression of Toll-like Receptors in Chicken Monocytes |
| title_full_unstemmed | Mannose Receptor Ligands Regulate the Gene Expression of Toll-like Receptors in Chicken Monocytes |
| title_short | Mannose Receptor Ligands Regulate the Gene Expression of Toll-like Receptors in Chicken Monocytes |
| title_sort | mannose receptor ligands regulate the gene expression of toll like receptors in chicken monocytes |
| topic | chicken mannose receptor monocytes toll-like receptor |
| url | https://www.jstage.jst.go.jp/article/jpsa/50/4/50_0120178/_pdf/-char/en |
| work_keys_str_mv | AT liyan mannosereceptorligandsregulatethegeneexpressionoftolllikereceptorsinchickenmonocytes AT zandongli mannosereceptorligandsregulatethegeneexpressionoftolllikereceptorsinchickenmonocytes |