Effect of sugar transporter on galactose utilization in Streptococcus thermophilus
IntroductionStreptococcus thermophilus is a traditional starter for dairy products. The lactose rich in milk is the main carbon source for the growth of S. thermophilus. However, the utilization of galactose by S. thermophilus is strain-specific, and many genetic factors can affect the sugar utiliza...
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1267237/full |
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| author | Jiancun Zhao Yan Liang Yan Liang Susu Zhang Zhenshang Xu Zhenshang Xu |
| author_facet | Jiancun Zhao Yan Liang Yan Liang Susu Zhang Zhenshang Xu Zhenshang Xu |
| author_sort | Jiancun Zhao |
| collection | DOAJ |
| description | IntroductionStreptococcus thermophilus is a traditional starter for dairy products. The lactose rich in milk is the main carbon source for the growth of S. thermophilus. However, the utilization of galactose by S. thermophilus is strain-specific, and many genetic factors can affect the sugar utilization phenotype of S. thermophilus strains.MethodsIn this study, S. thermophilus A25, which is capable of utilizing lactose and galactose, was used as the starting strain to construct lactose permease-deficient mutant S. thermophilus ΔlacS. Subsequently, the complement vectors expressing complete lactose permease of S. thermophilus and its N-terminal 1–486 amino acid residues were constructed and transformed into S. thermophilus ΔlacS, respectively. Meanwhile, complement vectors expressing lactose permease and galactose/proton symporter of Escherichia coli were also constructed.Results and DiscussionResults showed that S. thermophilus ΔlacS lost the ability to utilize lactose and galactose. By measuring the growth of the recombinant strains, it was found that the strain expressing complete lactose permease of S. thermophilus recovered the growth ability in lactose and galactose medium, while the strain expressing N-terminal of lactose permease recovered the growth ability only in lactose medium. Furthermore, the transformation of S. thermophilus ΔlacS was not successful with the complement vector expressing E. coli lactose permease, while the strain expressing E. coli galactose/proton symporter could recover its growth ability in the galactose medium. These results suggest that the properties of sugar transporters play an important role in galactose utilization by S. thermophilus. |
| first_indexed | 2024-03-10T00:07:10Z |
| format | Article |
| id | doaj.art-6780df6072464a07bb0f7036508ce2ca |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-10T00:07:10Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6780df6072464a07bb0f7036508ce2ca2023-11-23T16:06:00ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-11-011410.3389/fmicb.2023.12672371267237Effect of sugar transporter on galactose utilization in Streptococcus thermophilusJiancun Zhao0Yan Liang1Yan Liang2Susu Zhang3Zhenshang Xu4Zhenshang Xu5Department of Pharmacy and Biotechnology, Zibo Vocational Institute, Zibo, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Science, Jinan, ChinaSchool of Bioengineering, Qilu University of Technology, Shandong Academy of Science, Jinan, ChinaCollege of Life Science, Shandong Normal University, Jinan, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Science, Jinan, ChinaSchool of Bioengineering, Qilu University of Technology, Shandong Academy of Science, Jinan, ChinaIntroductionStreptococcus thermophilus is a traditional starter for dairy products. The lactose rich in milk is the main carbon source for the growth of S. thermophilus. However, the utilization of galactose by S. thermophilus is strain-specific, and many genetic factors can affect the sugar utilization phenotype of S. thermophilus strains.MethodsIn this study, S. thermophilus A25, which is capable of utilizing lactose and galactose, was used as the starting strain to construct lactose permease-deficient mutant S. thermophilus ΔlacS. Subsequently, the complement vectors expressing complete lactose permease of S. thermophilus and its N-terminal 1–486 amino acid residues were constructed and transformed into S. thermophilus ΔlacS, respectively. Meanwhile, complement vectors expressing lactose permease and galactose/proton symporter of Escherichia coli were also constructed.Results and DiscussionResults showed that S. thermophilus ΔlacS lost the ability to utilize lactose and galactose. By measuring the growth of the recombinant strains, it was found that the strain expressing complete lactose permease of S. thermophilus recovered the growth ability in lactose and galactose medium, while the strain expressing N-terminal of lactose permease recovered the growth ability only in lactose medium. Furthermore, the transformation of S. thermophilus ΔlacS was not successful with the complement vector expressing E. coli lactose permease, while the strain expressing E. coli galactose/proton symporter could recover its growth ability in the galactose medium. These results suggest that the properties of sugar transporters play an important role in galactose utilization by S. thermophilus.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1267237/fullStreptococcus thermophilussugar transportergalactose utilizationlactose permeaselactose utilization |
| spellingShingle | Jiancun Zhao Yan Liang Yan Liang Susu Zhang Zhenshang Xu Zhenshang Xu Effect of sugar transporter on galactose utilization in Streptococcus thermophilus Frontiers in Microbiology Streptococcus thermophilus sugar transporter galactose utilization lactose permease lactose utilization |
| title | Effect of sugar transporter on galactose utilization in Streptococcus thermophilus |
| title_full | Effect of sugar transporter on galactose utilization in Streptococcus thermophilus |
| title_fullStr | Effect of sugar transporter on galactose utilization in Streptococcus thermophilus |
| title_full_unstemmed | Effect of sugar transporter on galactose utilization in Streptococcus thermophilus |
| title_short | Effect of sugar transporter on galactose utilization in Streptococcus thermophilus |
| title_sort | effect of sugar transporter on galactose utilization in streptococcus thermophilus |
| topic | Streptococcus thermophilus sugar transporter galactose utilization lactose permease lactose utilization |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1267237/full |
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