Application of DNA Replicons in Gene Therapy and Vaccine Development
DNA-based gene therapy and vaccine development has received plenty of attention lately. DNA replicons based on self-replicating RNA viruses such as alphaviruses and flaviviruses have been of particular interest due to the amplification of RNA transcripts leading to enhanced transgene expression in t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/3/947 |
| _version_ | 1797609498362249216 |
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| author | Kenneth Lundstrom |
| author_facet | Kenneth Lundstrom |
| author_sort | Kenneth Lundstrom |
| collection | DOAJ |
| description | DNA-based gene therapy and vaccine development has received plenty of attention lately. DNA replicons based on self-replicating RNA viruses such as alphaviruses and flaviviruses have been of particular interest due to the amplification of RNA transcripts leading to enhanced transgene expression in transfected host cells. Moreover, significantly reduced doses of DNA replicons compared to conventional DNA plasmids can elicit equivalent immune responses. DNA replicons have been evaluated in preclinical animal models for cancer immunotherapy and for vaccines against infectious diseases and various cancers. Strong immune responses and tumor regression have been obtained in rodent tumor models. Immunization with DNA replicons has provided robust immune responses and protection against challenges with pathogens and tumor cells. DNA replicon-based COVID-19 vaccines have shown positive results in preclinical animal models. |
| first_indexed | 2024-03-11T06:02:12Z |
| format | Article |
| id | doaj.art-678703d85a554edca043f6488fbada51 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T06:02:12Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-678703d85a554edca043f6488fbada512023-11-17T13:16:38ZengMDPI AGPharmaceutics1999-49232023-03-0115394710.3390/pharmaceutics15030947Application of DNA Replicons in Gene Therapy and Vaccine DevelopmentKenneth Lundstrom0PanTherapeutics, CH1095 Lutry, SwitzerlandDNA-based gene therapy and vaccine development has received plenty of attention lately. DNA replicons based on self-replicating RNA viruses such as alphaviruses and flaviviruses have been of particular interest due to the amplification of RNA transcripts leading to enhanced transgene expression in transfected host cells. Moreover, significantly reduced doses of DNA replicons compared to conventional DNA plasmids can elicit equivalent immune responses. DNA replicons have been evaluated in preclinical animal models for cancer immunotherapy and for vaccines against infectious diseases and various cancers. Strong immune responses and tumor regression have been obtained in rodent tumor models. Immunization with DNA replicons has provided robust immune responses and protection against challenges with pathogens and tumor cells. DNA replicon-based COVID-19 vaccines have shown positive results in preclinical animal models.https://www.mdpi.com/1999-4923/15/3/947DNA repliconsself-replication RNA virusesRNA amplificationalphavirusesflavivirusescancer gene therapy |
| spellingShingle | Kenneth Lundstrom Application of DNA Replicons in Gene Therapy and Vaccine Development Pharmaceutics DNA replicons self-replication RNA viruses RNA amplification alphaviruses flaviviruses cancer gene therapy |
| title | Application of DNA Replicons in Gene Therapy and Vaccine Development |
| title_full | Application of DNA Replicons in Gene Therapy and Vaccine Development |
| title_fullStr | Application of DNA Replicons in Gene Therapy and Vaccine Development |
| title_full_unstemmed | Application of DNA Replicons in Gene Therapy and Vaccine Development |
| title_short | Application of DNA Replicons in Gene Therapy and Vaccine Development |
| title_sort | application of dna replicons in gene therapy and vaccine development |
| topic | DNA replicons self-replication RNA viruses RNA amplification alphaviruses flaviviruses cancer gene therapy |
| url | https://www.mdpi.com/1999-4923/15/3/947 |
| work_keys_str_mv | AT kennethlundstrom applicationofdnarepliconsingenetherapyandvaccinedevelopment |