Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginseng
Background: The present study aimed to compare the relative abundance of proteins and amino acid metabolites to explore the mechanisms underlying the difference between wild and cultivated ginseng (Panax ginseng Meyer) at the amino acid level. Methods: Two-dimensional polyacrylamide gel electrophore...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-04-01
|
| Series: | Journal of Ginseng Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845315000512 |
| _version_ | 1828778755205627904 |
|---|---|
| author | Hang Sun Fangbing Liu Liwei Sun Jianzeng Liu Manying Wang Xuenan Chen Xiaohao Xu Rui Ma Kai Feng Rui Jiang |
| author_facet | Hang Sun Fangbing Liu Liwei Sun Jianzeng Liu Manying Wang Xuenan Chen Xiaohao Xu Rui Ma Kai Feng Rui Jiang |
| author_sort | Hang Sun |
| collection | DOAJ |
| description | Background: The present study aimed to compare the relative abundance of proteins and amino acid metabolites to explore the mechanisms underlying the difference between wild and cultivated ginseng (Panax ginseng Meyer) at the amino acid level.
Methods: Two-dimensional polyacrylamide gel electrophoresis and isobaric tags for relative and absolute quantitation were used to identify the differential abundance of proteins between wild and cultivated ginseng. Total amino acids in wild and cultivated ginseng were compared using an automated amino acid analyzer. The activities of amino acid metabolism-related enzymes and the contents of intermediate metabolites between wild and cultivated ginseng were measured using enzyme-linked immunosorbent assay and spectrophotometric methods.
Results: Our results showed that the contents of 14 types of amino acids were higher in wild ginseng compared with cultivated ginseng. The amino acid metabolism-related enzymes and their derivatives, such as glutamate decarboxylase and S-adenosylmethionine, all had high levels of accumulation in wild ginseng. The accumulation of sulfur amino acid synthesis-related proteins, such as methionine synthase, was also higher in wild ginseng. In addition, glycolysis and tricarboxylic acid cycle-related enzymes as well as their intermediates had high levels of accumulation in wild ginseng.
Conclusion: This study elucidates the differences in amino acids between wild and cultivated ginseng. These results will provide a reference for further studies on the medicinal functions of wild ginseng. |
| first_indexed | 2024-12-11T16:49:54Z |
| format | Article |
| id | doaj.art-678b15a271614006901f029d44fe2adf |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-12-11T16:49:54Z |
| publishDate | 2016-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-678b15a271614006901f029d44fe2adf2022-12-22T00:58:08ZengElsevierJournal of Ginseng Research1226-84532016-04-0140211312010.1016/j.jgr.2015.06.001Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginsengHang Sun0Fangbing Liu1Liwei Sun2Jianzeng Liu3Manying Wang4Xuenan Chen5Xiaohao Xu6Rui Ma7Kai Feng8Rui Jiang9Jilin Technology Innovation Center for Chinese Medicine Biotechnology, Beihua University, Jilin Province, PR ChinaCollege of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, Jiangsu Province, PR ChinaJilin Technology Innovation Center for Chinese Medicine Biotechnology, Beihua University, Jilin Province, PR ChinaJilin Technology Innovation Center for Chinese Medicine Biotechnology, Beihua University, Jilin Province, PR ChinaJilin Technology Innovation Center for Chinese Medicine Biotechnology, Beihua University, Jilin Province, PR ChinaResearch Center of Traditional Chinese Medicine, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin Province, PR ChinaJilin Technology Innovation Center for Chinese Medicine Biotechnology, Beihua University, Jilin Province, PR ChinaJilin Technology Innovation Center for Chinese Medicine Biotechnology, Beihua University, Jilin Province, PR ChinaJilin Technology Innovation Center for Chinese Medicine Biotechnology, Beihua University, Jilin Province, PR ChinaJilin Technology Innovation Center for Chinese Medicine Biotechnology, Beihua University, Jilin Province, PR ChinaBackground: The present study aimed to compare the relative abundance of proteins and amino acid metabolites to explore the mechanisms underlying the difference between wild and cultivated ginseng (Panax ginseng Meyer) at the amino acid level. Methods: Two-dimensional polyacrylamide gel electrophoresis and isobaric tags for relative and absolute quantitation were used to identify the differential abundance of proteins between wild and cultivated ginseng. Total amino acids in wild and cultivated ginseng were compared using an automated amino acid analyzer. The activities of amino acid metabolism-related enzymes and the contents of intermediate metabolites between wild and cultivated ginseng were measured using enzyme-linked immunosorbent assay and spectrophotometric methods. Results: Our results showed that the contents of 14 types of amino acids were higher in wild ginseng compared with cultivated ginseng. The amino acid metabolism-related enzymes and their derivatives, such as glutamate decarboxylase and S-adenosylmethionine, all had high levels of accumulation in wild ginseng. The accumulation of sulfur amino acid synthesis-related proteins, such as methionine synthase, was also higher in wild ginseng. In addition, glycolysis and tricarboxylic acid cycle-related enzymes as well as their intermediates had high levels of accumulation in wild ginseng. Conclusion: This study elucidates the differences in amino acids between wild and cultivated ginseng. These results will provide a reference for further studies on the medicinal functions of wild ginseng.http://www.sciencedirect.com/science/article/pii/S1226845315000512amino acid metabolismcultivated ginsengPanax ginseng Meyerproteomicswild ginseng |
| spellingShingle | Hang Sun Fangbing Liu Liwei Sun Jianzeng Liu Manying Wang Xuenan Chen Xiaohao Xu Rui Ma Kai Feng Rui Jiang Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginseng Journal of Ginseng Research amino acid metabolism cultivated ginseng Panax ginseng Meyer proteomics wild ginseng |
| title | Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginseng |
| title_full | Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginseng |
| title_fullStr | Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginseng |
| title_full_unstemmed | Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginseng |
| title_short | Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginseng |
| title_sort | proteomic analysis of amino acid metabolism differences between wild and cultivated panax ginseng |
| topic | amino acid metabolism cultivated ginseng Panax ginseng Meyer proteomics wild ginseng |
| url | http://www.sciencedirect.com/science/article/pii/S1226845315000512 |
| work_keys_str_mv | AT hangsun proteomicanalysisofaminoacidmetabolismdifferencesbetweenwildandcultivatedpanaxginseng AT fangbingliu proteomicanalysisofaminoacidmetabolismdifferencesbetweenwildandcultivatedpanaxginseng AT liweisun proteomicanalysisofaminoacidmetabolismdifferencesbetweenwildandcultivatedpanaxginseng AT jianzengliu proteomicanalysisofaminoacidmetabolismdifferencesbetweenwildandcultivatedpanaxginseng AT manyingwang proteomicanalysisofaminoacidmetabolismdifferencesbetweenwildandcultivatedpanaxginseng AT xuenanchen proteomicanalysisofaminoacidmetabolismdifferencesbetweenwildandcultivatedpanaxginseng AT xiaohaoxu proteomicanalysisofaminoacidmetabolismdifferencesbetweenwildandcultivatedpanaxginseng AT ruima proteomicanalysisofaminoacidmetabolismdifferencesbetweenwildandcultivatedpanaxginseng AT kaifeng proteomicanalysisofaminoacidmetabolismdifferencesbetweenwildandcultivatedpanaxginseng AT ruijiang proteomicanalysisofaminoacidmetabolismdifferencesbetweenwildandcultivatedpanaxginseng |