Reference interval, longitudinal variability and reliability of activated clotting time in healthy dogs using a point‐of‐care analyser
Abstract Background Activated clotting times (ACTs) are used to screen for coagulopathies and monitor heparin therapy. Objectives To determine a reference interval (RI) for ACT in dogs using a point‐of‐care analyser, to quantify intra‐subject within‐ and between‐day variability, to quantify analyser...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-07-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.1148 |
| _version_ | 1797776591139373056 |
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| author | Arnaut Hellemans Nausikaa Devriendt Filip De Somer Sofie Marynissen Sylvie Daminet Dominique Paepe Pascale Smets |
| author_facet | Arnaut Hellemans Nausikaa Devriendt Filip De Somer Sofie Marynissen Sylvie Daminet Dominique Paepe Pascale Smets |
| author_sort | Arnaut Hellemans |
| collection | DOAJ |
| description | Abstract Background Activated clotting times (ACTs) are used to screen for coagulopathies and monitor heparin therapy. Objectives To determine a reference interval (RI) for ACT in dogs using a point‐of‐care analyser, to quantify intra‐subject within‐ and between‐day variability, to quantify analyser reliability and inter‐analyser agreement and to study the influence of a delay in measurement. Methods Forty‐two healthy dogs were included. Measurements were performed on fresh venous blood using the i‐STAT 1 analyser. The RI was determined using the Robust method. Intra‐subject within‐day variability and between‐day variability were quantified between baseline and 2 h (n = 8) or 48 h (n = 10) later. Analyser reliability and inter‐analyser agreement were studied by duplicate measurements (n = 8) on identical analysers. The influence of measurement delay was studied before and after a delay of one analytical run (n = 6). Results Mean, lower and upper reference limits for ACT were 92.9 ± 9.1, 74.4 and 111.2 s, respectively. Coefficients of variation of intra‐subject within‐ and between‐day variability were 8.1% and 10.4%, respectively, resulting in a significant between‐day measurement difference. Analyser reliability assessed by the intraclass correlation coefficient and coefficient of variation were 0.87% and 3.3%, respectively. Significantly lower ACT values were observed after a measurement delay compared to direct analysis. Conclusions Our study provides an RI for ACT in healthy dogs using the i‐STAT 1 and suggests low intra‐subject within‐ and between‐day variability. Analyser reliability and inter‐analyser agreement were good; however, analysis delay and between‐day differences could significantly influence ACT results. |
| first_indexed | 2024-03-12T22:52:01Z |
| format | Article |
| id | doaj.art-6790223fe1934a1fb620ae181f021a32 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-03-12T22:52:01Z |
| publishDate | 2023-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-6790223fe1934a1fb620ae181f021a322023-07-20T08:09:46ZengWileyVeterinary Medicine and Science2053-10952023-07-01941534154010.1002/vms3.1148Reference interval, longitudinal variability and reliability of activated clotting time in healthy dogs using a point‐of‐care analyserArnaut Hellemans0Nausikaa Devriendt1Filip De Somer2Sofie Marynissen3Sylvie Daminet4Dominique Paepe5Pascale Smets6Small Animal Department, Faculty of Veterinary Medicine Ghent University Merelbeke BelgiumSmall Animal Department, Faculty of Veterinary Medicine Ghent University Merelbeke BelgiumDepartment of Cardiac Surgery, Faculty of Medical Sciences Ghent University Ghent BelgiumSmall Animal Department, Faculty of Veterinary Medicine Ghent University Merelbeke BelgiumSmall Animal Department, Faculty of Veterinary Medicine Ghent University Merelbeke BelgiumSmall Animal Department, Faculty of Veterinary Medicine Ghent University Merelbeke BelgiumSmall Animal Department, Faculty of Veterinary Medicine Ghent University Merelbeke BelgiumAbstract Background Activated clotting times (ACTs) are used to screen for coagulopathies and monitor heparin therapy. Objectives To determine a reference interval (RI) for ACT in dogs using a point‐of‐care analyser, to quantify intra‐subject within‐ and between‐day variability, to quantify analyser reliability and inter‐analyser agreement and to study the influence of a delay in measurement. Methods Forty‐two healthy dogs were included. Measurements were performed on fresh venous blood using the i‐STAT 1 analyser. The RI was determined using the Robust method. Intra‐subject within‐day variability and between‐day variability were quantified between baseline and 2 h (n = 8) or 48 h (n = 10) later. Analyser reliability and inter‐analyser agreement were studied by duplicate measurements (n = 8) on identical analysers. The influence of measurement delay was studied before and after a delay of one analytical run (n = 6). Results Mean, lower and upper reference limits for ACT were 92.9 ± 9.1, 74.4 and 111.2 s, respectively. Coefficients of variation of intra‐subject within‐ and between‐day variability were 8.1% and 10.4%, respectively, resulting in a significant between‐day measurement difference. Analyser reliability assessed by the intraclass correlation coefficient and coefficient of variation were 0.87% and 3.3%, respectively. Significantly lower ACT values were observed after a measurement delay compared to direct analysis. Conclusions Our study provides an RI for ACT in healthy dogs using the i‐STAT 1 and suggests low intra‐subject within‐ and between‐day variability. Analyser reliability and inter‐analyser agreement were good; however, analysis delay and between‐day differences could significantly influence ACT results.https://doi.org/10.1002/vms3.1148bedside testingcaninecoagulationheparin monitoringi‐STAT 1screening |
| spellingShingle | Arnaut Hellemans Nausikaa Devriendt Filip De Somer Sofie Marynissen Sylvie Daminet Dominique Paepe Pascale Smets Reference interval, longitudinal variability and reliability of activated clotting time in healthy dogs using a point‐of‐care analyser Veterinary Medicine and Science bedside testing canine coagulation heparin monitoring i‐STAT 1 screening |
| title | Reference interval, longitudinal variability and reliability of activated clotting time in healthy dogs using a point‐of‐care analyser |
| title_full | Reference interval, longitudinal variability and reliability of activated clotting time in healthy dogs using a point‐of‐care analyser |
| title_fullStr | Reference interval, longitudinal variability and reliability of activated clotting time in healthy dogs using a point‐of‐care analyser |
| title_full_unstemmed | Reference interval, longitudinal variability and reliability of activated clotting time in healthy dogs using a point‐of‐care analyser |
| title_short | Reference interval, longitudinal variability and reliability of activated clotting time in healthy dogs using a point‐of‐care analyser |
| title_sort | reference interval longitudinal variability and reliability of activated clotting time in healthy dogs using a point of care analyser |
| topic | bedside testing canine coagulation heparin monitoring i‐STAT 1 screening |
| url | https://doi.org/10.1002/vms3.1148 |
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