Determination of kanamycin residue in anatolian buffalo milk by lc-ms/ms
The present study aimed to evaluate the persistence of kanamycin in lactating Anatolian buffalo milk followed by an intramuscular injection of kanamycin. The collection of milk samples was performed twice daily up to the 10th milking followed by kanamycin injection and liquid chromatography coupled...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2019-08-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2636 |
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| author | Ulas ACAROZ Sinan INCE Damla ARSLAN-ACAROZ Ismail KUCUKKURT Abdullah ERYAVUZ |
| author_facet | Ulas ACAROZ Sinan INCE Damla ARSLAN-ACAROZ Ismail KUCUKKURT Abdullah ERYAVUZ |
| author_sort | Ulas ACAROZ |
| collection | DOAJ |
| description | The present study aimed to evaluate the persistence of kanamycin in lactating Anatolian buffalo milk followed by an intramuscular injection
of kanamycin. The collection of milk samples was performed twice daily up to the 10th milking followed by kanamycin injection and liquid
chromatography coupled with tandem mass spectrometry (LC-MS/MS) was employed for the residue analysis. The detection limit of the
method was determined as 3.56 μg/kg. The highest concentrations of kanamycin were determined in the first milking after injection and
mean concentration of this milking was found to be as 1473 μg/kg. Kanamycin residue in all buffalo milk samples was lower than the
maximum residue limit (150 μg/kg) with the fifth milking. In addition, a monitoring study was conducted to determine whether buffalo milk
marketed in Afyonkarahisar pose a public health risk regarding kanamycin residue. The results of the monitoring study showed that one (2%) of
the 50 buffalo milk samples contained kanamycin at the concentration of 22.36 μg/kg. In conclusion, this study determined the persistence
of kanamycin in Anatolian buffalo milks based on an LC-MS/MS method. In addition, results of the study showed that buffalo milks marketed
in Afyonkarahisar Province pose a very low risk regarding kanamycin residue. |
| first_indexed | 2024-03-13T04:38:20Z |
| format | Article |
| id | doaj.art-67914d04916c4e6bb0050dcf31216cfc |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:38:20Z |
| publishDate | 2019-08-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-67914d04916c4e6bb0050dcf31216cfc2023-06-19T06:33:13ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512019-08-012619710210.9775/kvfd.2019.224012636Determination of kanamycin residue in anatolian buffalo milk by lc-ms/msUlas ACAROZ0Sinan INCE1Damla ARSLAN-ACAROZ2Ismail KUCUKKURT3Abdullah ERYAVUZ4Afyon Kocatepe University, Veterinary Faculty, Department of Food Hygiene and Technology, TR-03200 Afyonkarahisar - TURKEYAfyon Kocatepe University, Veterinary Faculty, Department of Pharmacology and Toxicology, TR-03200 Afyonkarahisar - TURKEYAfyon Kocatepe University, Bayat Vocational School, TR-03780 Bayat, Afyonkarahisar - TURKEYAfyon Kocatepe University, Veterinary Faculty, Department of Biochemistry, TR-03200 Afyonkarahisar - TURKEYAfyon Kocatepe University, Veterinary Faculty, Department of Physiology, TR-03200 Afyonkarahisar - TURKEYThe present study aimed to evaluate the persistence of kanamycin in lactating Anatolian buffalo milk followed by an intramuscular injection of kanamycin. The collection of milk samples was performed twice daily up to the 10th milking followed by kanamycin injection and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was employed for the residue analysis. The detection limit of the method was determined as 3.56 μg/kg. The highest concentrations of kanamycin were determined in the first milking after injection and mean concentration of this milking was found to be as 1473 μg/kg. Kanamycin residue in all buffalo milk samples was lower than the maximum residue limit (150 μg/kg) with the fifth milking. In addition, a monitoring study was conducted to determine whether buffalo milk marketed in Afyonkarahisar pose a public health risk regarding kanamycin residue. The results of the monitoring study showed that one (2%) of the 50 buffalo milk samples contained kanamycin at the concentration of 22.36 μg/kg. In conclusion, this study determined the persistence of kanamycin in Anatolian buffalo milks based on an LC-MS/MS method. In addition, results of the study showed that buffalo milks marketed in Afyonkarahisar Province pose a very low risk regarding kanamycin residue.https://vetdergikafkas.org/pdf.php?id=2636kanamycinaminoglycosidesanatolian buffaloesmilklc-ms/ms |
| spellingShingle | Ulas ACAROZ Sinan INCE Damla ARSLAN-ACAROZ Ismail KUCUKKURT Abdullah ERYAVUZ Determination of kanamycin residue in anatolian buffalo milk by lc-ms/ms Kafkas Universitesi Veteriner Fakültesi Dergisi kanamycin aminoglycosides anatolian buffaloes milk lc-ms/ms |
| title | Determination of kanamycin residue in anatolian buffalo milk by lc-ms/ms |
| title_full | Determination of kanamycin residue in anatolian buffalo milk by lc-ms/ms |
| title_fullStr | Determination of kanamycin residue in anatolian buffalo milk by lc-ms/ms |
| title_full_unstemmed | Determination of kanamycin residue in anatolian buffalo milk by lc-ms/ms |
| title_short | Determination of kanamycin residue in anatolian buffalo milk by lc-ms/ms |
| title_sort | determination of kanamycin residue in anatolian buffalo milk by lc ms ms |
| topic | kanamycin aminoglycosides anatolian buffaloes milk lc-ms/ms |
| url | https://vetdergikafkas.org/pdf.php?id=2636 |
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