Melatonin in preservation solutions prevents ischemic injury in rat kidneys.
Transplantation is lifesaving and the most effective treatment for end-stage organ failure. The transplantation success depends on the functional preservation of organs prior to transplantation. Currently, the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) are the most com...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0273921 |
| _version_ | 1818023009981562880 |
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| author | Abdurrahman Coskun Cumhur Yegen Serap Arbak Wafi Attaallah Omer Gunal Merve Acikel Elmas Yasemin Ucal Ozge Can Banu Baş Zeynep Yildirim Ismail Seckin Sibel Demirci Mustafa Serteser Aysel Ozpinar Ahmet Belce Gulcin Basdemir Derya Emel Moldur Ecenur Izzete Derelioglu Tahir Koray Yozgatli Yigit Erdemgil Ibrahim Unsal |
| author_facet | Abdurrahman Coskun Cumhur Yegen Serap Arbak Wafi Attaallah Omer Gunal Merve Acikel Elmas Yasemin Ucal Ozge Can Banu Baş Zeynep Yildirim Ismail Seckin Sibel Demirci Mustafa Serteser Aysel Ozpinar Ahmet Belce Gulcin Basdemir Derya Emel Moldur Ecenur Izzete Derelioglu Tahir Koray Yozgatli Yigit Erdemgil Ibrahim Unsal |
| author_sort | Abdurrahman Coskun |
| collection | DOAJ |
| description | Transplantation is lifesaving and the most effective treatment for end-stage organ failure. The transplantation success depends on the functional preservation of organs prior to transplantation. Currently, the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) are the most commonly used preservation solutions. Despite intensive efforts, the functional preservation of solid organs prior to transplantation is limited to hours. In this study, we modified the UW solution containing components from both the UW and HTK solutions and analyzed their tissue-protective effect against ischemic injury. The composition of the UW solution was changed by reducing hydroxyethyl starch concentration and adding Histidine/Histidine-HCl which is the main component of HTK solution. Additionally, the preservation solutions were supplemented with melatonin and glucosamine. The protective effects of the preservation solutions were assessed by biochemical and microscopical analysis at 2, 10, 24, and 72 h after preserving the rat kidneys with static cold storage. Lactate dehydrogenase (LDH) activity in preservation solutions was measured at 2, 10, 24, and 72. It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups. At the 72nd hour, the lowest LDH activity (0.91 IU/g (0.63-1.17)) was measured in the mUW-m group. In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours. The mUW-m solution at low temperature was an effective and suitable solution to protect renal tissue for up to 72 h. |
| first_indexed | 2024-12-10T03:37:31Z |
| format | Article |
| id | doaj.art-67922badc3324c8194aa966886f94834 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T03:37:31Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-67922badc3324c8194aa966886f948342022-12-22T02:03:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01178e027392110.1371/journal.pone.0273921Melatonin in preservation solutions prevents ischemic injury in rat kidneys.Abdurrahman CoskunCumhur YegenSerap ArbakWafi AttaallahOmer GunalMerve Acikel ElmasYasemin UcalOzge CanBanu BaşZeynep YildirimIsmail SeckinSibel DemirciMustafa SerteserAysel OzpinarAhmet BelceGulcin BasdemirDerya Emel MoldurEcenur Izzete DereliogluTahir Koray YozgatliYigit ErdemgilIbrahim UnsalTransplantation is lifesaving and the most effective treatment for end-stage organ failure. The transplantation success depends on the functional preservation of organs prior to transplantation. Currently, the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) are the most commonly used preservation solutions. Despite intensive efforts, the functional preservation of solid organs prior to transplantation is limited to hours. In this study, we modified the UW solution containing components from both the UW and HTK solutions and analyzed their tissue-protective effect against ischemic injury. The composition of the UW solution was changed by reducing hydroxyethyl starch concentration and adding Histidine/Histidine-HCl which is the main component of HTK solution. Additionally, the preservation solutions were supplemented with melatonin and glucosamine. The protective effects of the preservation solutions were assessed by biochemical and microscopical analysis at 2, 10, 24, and 72 h after preserving the rat kidneys with static cold storage. Lactate dehydrogenase (LDH) activity in preservation solutions was measured at 2, 10, 24, and 72. It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups. At the 72nd hour, the lowest LDH activity (0.91 IU/g (0.63-1.17)) was measured in the mUW-m group. In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours. The mUW-m solution at low temperature was an effective and suitable solution to protect renal tissue for up to 72 h.https://doi.org/10.1371/journal.pone.0273921 |
| spellingShingle | Abdurrahman Coskun Cumhur Yegen Serap Arbak Wafi Attaallah Omer Gunal Merve Acikel Elmas Yasemin Ucal Ozge Can Banu Baş Zeynep Yildirim Ismail Seckin Sibel Demirci Mustafa Serteser Aysel Ozpinar Ahmet Belce Gulcin Basdemir Derya Emel Moldur Ecenur Izzete Derelioglu Tahir Koray Yozgatli Yigit Erdemgil Ibrahim Unsal Melatonin in preservation solutions prevents ischemic injury in rat kidneys. PLoS ONE |
| title | Melatonin in preservation solutions prevents ischemic injury in rat kidneys. |
| title_full | Melatonin in preservation solutions prevents ischemic injury in rat kidneys. |
| title_fullStr | Melatonin in preservation solutions prevents ischemic injury in rat kidneys. |
| title_full_unstemmed | Melatonin in preservation solutions prevents ischemic injury in rat kidneys. |
| title_short | Melatonin in preservation solutions prevents ischemic injury in rat kidneys. |
| title_sort | melatonin in preservation solutions prevents ischemic injury in rat kidneys |
| url | https://doi.org/10.1371/journal.pone.0273921 |
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