Micro-computed tomography assessment of bone structure in aging mice
Abstract High-resolution computed tomography (CT) is widely used to assess bone structure under physiological and pathological conditions. Although the analytic protocols and parameters for micro-CT (μCT) analyses in mice are standardized for long bones, vertebrae, and the palms in aging mice, they...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-11965-4 |
| _version_ | 1811248881605804032 |
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| author | Junbo Shim Chihiro Iwaya Catherine G. Ambrose Akiko Suzuki Junichi Iwata |
| author_facet | Junbo Shim Chihiro Iwaya Catherine G. Ambrose Akiko Suzuki Junichi Iwata |
| author_sort | Junbo Shim |
| collection | DOAJ |
| description | Abstract High-resolution computed tomography (CT) is widely used to assess bone structure under physiological and pathological conditions. Although the analytic protocols and parameters for micro-CT (μCT) analyses in mice are standardized for long bones, vertebrae, and the palms in aging mice, they have not yet been established for craniofacial bones. In this study, we conducted a morphometric assessment of craniofacial bones, in comparison with long bones, in aging mice. Although age-related changes were observed in the microarchitecture of the femur, tibia, vertebra, and basisphenoid bone, and were more pronounced in females than in males, the microarchitecture of both the interparietal bone and body of the mandible, which develop by intramembranous ossification, was less affected by age and sex. By contrast, the condyle of the mandible was more affected by aging in males compared to females. Taken together, our results indicate that mouse craniofacial bones are uniquely affected by age and sex. |
| first_indexed | 2024-04-12T15:35:30Z |
| format | Article |
| id | doaj.art-6793ab69420b45e2be981c041109b0d1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T15:35:30Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6793ab69420b45e2be981c041109b0d12022-12-22T03:26:58ZengNature PortfolioScientific Reports2045-23222022-05-0112111610.1038/s41598-022-11965-4Micro-computed tomography assessment of bone structure in aging miceJunbo Shim0Chihiro Iwaya1Catherine G. Ambrose2Akiko Suzuki3Junichi Iwata4Department of Diagnostic and Biomedical Sciences, School of Dentistry, The University of Texas Health Science Center at HoustonDepartment of Diagnostic and Biomedical Sciences, School of Dentistry, The University of Texas Health Science Center at HoustonDepartment of Orthopedic Surgery, McGovern Medical School, The University of Texas Health Science Center at HoustonDepartment of Diagnostic and Biomedical Sciences, School of Dentistry, The University of Texas Health Science Center at HoustonDepartment of Diagnostic and Biomedical Sciences, School of Dentistry, The University of Texas Health Science Center at HoustonAbstract High-resolution computed tomography (CT) is widely used to assess bone structure under physiological and pathological conditions. Although the analytic protocols and parameters for micro-CT (μCT) analyses in mice are standardized for long bones, vertebrae, and the palms in aging mice, they have not yet been established for craniofacial bones. In this study, we conducted a morphometric assessment of craniofacial bones, in comparison with long bones, in aging mice. Although age-related changes were observed in the microarchitecture of the femur, tibia, vertebra, and basisphenoid bone, and were more pronounced in females than in males, the microarchitecture of both the interparietal bone and body of the mandible, which develop by intramembranous ossification, was less affected by age and sex. By contrast, the condyle of the mandible was more affected by aging in males compared to females. Taken together, our results indicate that mouse craniofacial bones are uniquely affected by age and sex.https://doi.org/10.1038/s41598-022-11965-4 |
| spellingShingle | Junbo Shim Chihiro Iwaya Catherine G. Ambrose Akiko Suzuki Junichi Iwata Micro-computed tomography assessment of bone structure in aging mice Scientific Reports |
| title | Micro-computed tomography assessment of bone structure in aging mice |
| title_full | Micro-computed tomography assessment of bone structure in aging mice |
| title_fullStr | Micro-computed tomography assessment of bone structure in aging mice |
| title_full_unstemmed | Micro-computed tomography assessment of bone structure in aging mice |
| title_short | Micro-computed tomography assessment of bone structure in aging mice |
| title_sort | micro computed tomography assessment of bone structure in aging mice |
| url | https://doi.org/10.1038/s41598-022-11965-4 |
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