Genetic ancestry and population structure of vaccinia virus
Abstract Vaccinia virus (VACV) was used for smallpox eradication, but its ultimate origin remains unknown. The genetic relationships among vaccine stocks are also poorly understood. We analyzed 63 vaccine strains with different origin, as well horsepox virus (HPXV). Results indicated the genetic div...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-08-01
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| Series: | npj Vaccines |
| Online Access: | https://doi.org/10.1038/s41541-022-00519-4 |
| _version_ | 1797642371327852544 |
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| author | Cristian Molteni Diego Forni Rachele Cagliani Mario Clerici Manuela Sironi |
| author_facet | Cristian Molteni Diego Forni Rachele Cagliani Mario Clerici Manuela Sironi |
| author_sort | Cristian Molteni |
| collection | DOAJ |
| description | Abstract Vaccinia virus (VACV) was used for smallpox eradication, but its ultimate origin remains unknown. The genetic relationships among vaccine stocks are also poorly understood. We analyzed 63 vaccine strains with different origin, as well horsepox virus (HPXV). Results indicated the genetic diversity of VACV is intermediate between variola and cowpox viruses, and that mutation contributed more than recombination to VACV evolution. STRUCTURE identified 9 contributing subpopulations and showed that the lowest drift was experienced by the ancestry components of Tian Tan and HPXV/Mütter/Mulford genomes. Subpopulations that experienced very strong drift include those that contributed the ancestry of MVA and IHD-W, in good agreement with the very long passage history of these vaccines. Another highly drifted population contributed the full ancestry of viruses sampled from human/cattle infections in Brazil and, partially, to IOC clones, strongly suggesting that the recurrent infections in Brazil derive from the spillback of IOC to the feral state. |
| first_indexed | 2024-03-11T13:59:07Z |
| format | Article |
| id | doaj.art-6793acc7732448eba0db9243e555a0c1 |
| institution | Directory Open Access Journal |
| issn | 2059-0105 |
| language | English |
| last_indexed | 2024-03-11T13:59:07Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Vaccines |
| spelling | doaj.art-6793acc7732448eba0db9243e555a0c12023-11-02T05:13:13ZengNature Portfolionpj Vaccines2059-01052022-08-01711910.1038/s41541-022-00519-4Genetic ancestry and population structure of vaccinia virusCristian Molteni0Diego Forni1Rachele Cagliani2Mario Clerici3Manuela Sironi4IRCCS E. MEDEA, BioinformaticsIRCCS E. MEDEA, BioinformaticsIRCCS E. MEDEA, BioinformaticsUniversity of MilanIRCCS E. MEDEA, BioinformaticsAbstract Vaccinia virus (VACV) was used for smallpox eradication, but its ultimate origin remains unknown. The genetic relationships among vaccine stocks are also poorly understood. We analyzed 63 vaccine strains with different origin, as well horsepox virus (HPXV). Results indicated the genetic diversity of VACV is intermediate between variola and cowpox viruses, and that mutation contributed more than recombination to VACV evolution. STRUCTURE identified 9 contributing subpopulations and showed that the lowest drift was experienced by the ancestry components of Tian Tan and HPXV/Mütter/Mulford genomes. Subpopulations that experienced very strong drift include those that contributed the ancestry of MVA and IHD-W, in good agreement with the very long passage history of these vaccines. Another highly drifted population contributed the full ancestry of viruses sampled from human/cattle infections in Brazil and, partially, to IOC clones, strongly suggesting that the recurrent infections in Brazil derive from the spillback of IOC to the feral state.https://doi.org/10.1038/s41541-022-00519-4 |
| spellingShingle | Cristian Molteni Diego Forni Rachele Cagliani Mario Clerici Manuela Sironi Genetic ancestry and population structure of vaccinia virus npj Vaccines |
| title | Genetic ancestry and population structure of vaccinia virus |
| title_full | Genetic ancestry and population structure of vaccinia virus |
| title_fullStr | Genetic ancestry and population structure of vaccinia virus |
| title_full_unstemmed | Genetic ancestry and population structure of vaccinia virus |
| title_short | Genetic ancestry and population structure of vaccinia virus |
| title_sort | genetic ancestry and population structure of vaccinia virus |
| url | https://doi.org/10.1038/s41541-022-00519-4 |
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