RNA-Seq profiling of circular RNAs in human colorectal Cancer liver metastasis and the potential biomarkers
Abstract In this study, the secondary sequencing was used to profile circRNA expression in the tissue samples from three CRC patients with liver metastasis and three matched CRC patients. After verified some candidates in another 40 CRC and CRC-m samples by qRT-PCR, we further demonstrated that circ...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | Molecular Cancer |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12943-018-0932-8 |
| _version_ | 1819136278243835904 |
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| author | Hanchen Xu Chunyan Wang Haiyan Song Yangxian Xu Guang Ji |
| author_facet | Hanchen Xu Chunyan Wang Haiyan Song Yangxian Xu Guang Ji |
| author_sort | Hanchen Xu |
| collection | DOAJ |
| description | Abstract In this study, the secondary sequencing was used to profile circRNA expression in the tissue samples from three CRC patients with liver metastasis and three matched CRC patients. After verified some candidates in another 40 CRC and CRC-m samples by qRT-PCR, we further demonstrated that circRNA_0001178 and circRNA_0000826 were significantly upregulated in CRC-m tissues, and both of them had the potential for diagnosing liver metastases from colorectal cancer. Finally, the networks of circRNA-miRNA-mRNA base on these two circRNAs were constructed respectively. This study showed that differentially expressed circRNAs were existed between the tissue samples from colorectal cancer patients with and without liver metastasis. And also suggested that circRNA_0001178 and circRNA_0000826 may serve as a potential diagnostic biomarker for liver metastases from colorectal cancer. |
| first_indexed | 2024-12-22T10:32:26Z |
| format | Article |
| id | doaj.art-67955a79c3fd4a6b9cdb24be15bf8c32 |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-12-22T10:32:26Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-67955a79c3fd4a6b9cdb24be15bf8c322022-12-21T18:29:17ZengBMCMolecular Cancer1476-45982019-01-011811610.1186/s12943-018-0932-8RNA-Seq profiling of circular RNAs in human colorectal Cancer liver metastasis and the potential biomarkersHanchen Xu0Chunyan Wang1Haiyan Song2Yangxian Xu3Guang Ji4Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese MedicineInstitute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese MedicineInstitute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese MedicineDepartment of General Surgery, Longhua Hospital, Shanghai University of Traditional Chinese MedicineInstitute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese MedicineAbstract In this study, the secondary sequencing was used to profile circRNA expression in the tissue samples from three CRC patients with liver metastasis and three matched CRC patients. After verified some candidates in another 40 CRC and CRC-m samples by qRT-PCR, we further demonstrated that circRNA_0001178 and circRNA_0000826 were significantly upregulated in CRC-m tissues, and both of them had the potential for diagnosing liver metastases from colorectal cancer. Finally, the networks of circRNA-miRNA-mRNA base on these two circRNAs were constructed respectively. This study showed that differentially expressed circRNAs were existed between the tissue samples from colorectal cancer patients with and without liver metastasis. And also suggested that circRNA_0001178 and circRNA_0000826 may serve as a potential diagnostic biomarker for liver metastases from colorectal cancer.http://link.springer.com/article/10.1186/s12943-018-0932-8Colorectal cancer liver metastasisCircular RNAs (circRNAs)RNA-sequencing (RNA-seq)Biomarker |
| spellingShingle | Hanchen Xu Chunyan Wang Haiyan Song Yangxian Xu Guang Ji RNA-Seq profiling of circular RNAs in human colorectal Cancer liver metastasis and the potential biomarkers Molecular Cancer Colorectal cancer liver metastasis Circular RNAs (circRNAs) RNA-sequencing (RNA-seq) Biomarker |
| title | RNA-Seq profiling of circular RNAs in human colorectal Cancer liver metastasis and the potential biomarkers |
| title_full | RNA-Seq profiling of circular RNAs in human colorectal Cancer liver metastasis and the potential biomarkers |
| title_fullStr | RNA-Seq profiling of circular RNAs in human colorectal Cancer liver metastasis and the potential biomarkers |
| title_full_unstemmed | RNA-Seq profiling of circular RNAs in human colorectal Cancer liver metastasis and the potential biomarkers |
| title_short | RNA-Seq profiling of circular RNAs in human colorectal Cancer liver metastasis and the potential biomarkers |
| title_sort | rna seq profiling of circular rnas in human colorectal cancer liver metastasis and the potential biomarkers |
| topic | Colorectal cancer liver metastasis Circular RNAs (circRNAs) RNA-sequencing (RNA-seq) Biomarker |
| url | http://link.springer.com/article/10.1186/s12943-018-0932-8 |
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