Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats
Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocyte...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2017-03-01
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| Series: | Acta Veterinaria |
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| Online Access: | https://doi.org/10.1515/acve-2017-0009 |
| _version_ | 1818407030470213632 |
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| author | Danilović Luković Jelena Korać Aleksandra Milošević Ivan Lužajić Tijana Milanović Zorana Kovačević Filipović Milica Radovanović Anita |
| author_facet | Danilović Luković Jelena Korać Aleksandra Milošević Ivan Lužajić Tijana Milanović Zorana Kovačević Filipović Milica Radovanović Anita |
| author_sort | Danilović Luković Jelena |
| collection | DOAJ |
| description | Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/oocytes was significantly lower in the hypothyroid group. No significant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly reflecting that these proteins were not involved in the oogonia/oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/oocytes altered with the advanced process of autophagy. |
| first_indexed | 2024-12-14T09:21:21Z |
| format | Article |
| id | doaj.art-6796683b782542a3b7e4e22c6b101355 |
| institution | Directory Open Access Journal |
| issn | 1820-7448 |
| language | English |
| last_indexed | 2024-12-14T09:21:21Z |
| publishDate | 2017-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Veterinaria |
| spelling | doaj.art-6796683b782542a3b7e4e22c6b1013552022-12-21T23:08:19ZengSciendoActa Veterinaria1820-74482017-03-016719210610.1515/acve-2017-0009Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn ratsDanilović Luković Jelena0Korać Aleksandra1Milošević Ivan2Lužajić Tijana3Milanović Zorana4Kovačević Filipović Milica5Radovanović Anita6State University of Novi Pazar, Department of Biomedical Sciences, Vuka Karadžića bb, 36300 Novi Pazar, Serbia University of Belgrade, Center for Electron Microscopy, Faculty of Biology, Studentski trg 16, 11000 Belgrade, Serbia University of Belgrade, Faculty of Veterinary Medicine, Bulevar oslobođenja 18, 11000 Belgrade, Serbia University of Belgrade, Faculty of Veterinary Medicine, Bulevar oslobođenja 18, 11000 Belgrade, Serbia University of Belgrade, Faculty of Veterinary Medicine, Bulevar oslobođenja 18, 11000 Belgrade, Serbia University of Belgrade, Faculty of Veterinary Medicine, Bulevar oslobođenja 18, 11000 Belgrade, Serbia University of Belgrade, Faculty of Veterinary Medicine, Bulevar oslobođenja 18, 11000 Belgrade, SerbiaInduced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/oocytes was significantly lower in the hypothyroid group. No significant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly reflecting that these proteins were not involved in the oogonia/oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/oocytes altered with the advanced process of autophagy.https://doi.org/10.1515/acve-2017-0009atg7caspase-3lc3meiosis prophase isecondary necrosis |
| spellingShingle | Danilović Luković Jelena Korać Aleksandra Milošević Ivan Lužajić Tijana Milanović Zorana Kovačević Filipović Milica Radovanović Anita Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats Acta Veterinaria atg7 caspase-3 lc3 meiosis prophase i secondary necrosis |
| title | Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats |
| title_full | Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats |
| title_fullStr | Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats |
| title_full_unstemmed | Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats |
| title_short | Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats |
| title_sort | z cells and oogonia oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats |
| topic | atg7 caspase-3 lc3 meiosis prophase i secondary necrosis |
| url | https://doi.org/10.1515/acve-2017-0009 |
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