Evaluation of Rapid Diagnosis of Candidemia by Agglutination Test Using Gold Nanoparticles
Background: Candidemia showed high morbidity and mortality and should be diagnosed immediately. The aim of this study was to provide a simple and rapid method for direct detection of Candida in the blood, using conjugated antibodies with gold nanoparticles by the agglutination method.Methods: In thi...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Isfahan University of Medical Sciences
2022-05-01
|
| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | https://jims.mui.ac.ir/article_17205_2b343ac0945c5d3072b2bfa91ed0420a.pdf |
| _version_ | 1797704118531260416 |
|---|---|
| author | Zainab Mussaie Hossein Yousofi-Darani Hossein Hashemi Iman Zareie Parvin Dehghan |
| author_facet | Zainab Mussaie Hossein Yousofi-Darani Hossein Hashemi Iman Zareie Parvin Dehghan |
| author_sort | Zainab Mussaie |
| collection | DOAJ |
| description | Background: Candidemia showed high morbidity and mortality and should be diagnosed immediately. The aim of this study was to provide a simple and rapid method for direct detection of Candida in the blood, using conjugated antibodies with gold nanoparticles by the agglutination method.Methods: In this study, yeast species isolated from 40 blood samples of patients tested by the BACTEC method were used. After identifying the etiologic agents by morphological and molecular methods, a mixture of yeast antigens was prepared. Briefly, a mixture of antigens was prepared from the Candida isolates detected from candidemia, and injected into healthy rabbit skin with complete adjuvant to produce antibodies. After injecting the incomplete adjuvant in four reminders, blood samples and serum were finally prepared from the rabbit. Antibody production and its efficacy were confirmed by ELISA method. Gold nanoparticles were chemically conjugated with antibodies. For rapid diagnosis of candidemia, the nanoparticle method was examined on the blood of four patients and blood contaminated with a certain number of Candida yeasts in the laboratory.Findings: Candida albicans was the most common etiologic agent of candidemia, followed by Candida glabrata and Candida parapsilosis complex. In determining the cut-off nanoparticle method, the least yeast detectable in the patient's blood was one yeast. The sensitivity and specificity of the nanoparticle method for detecting Candida in the blood of four patients compared to blood culture was 100%.Conclusion: Under our laboratory conditions, if testing 15 µl blood of a suspected patient to candidemia containing one yeast or even just Candida antigens exposed to 35 microliters of the nanoparticles conjugated antibodies, it shows agglutination during one minute. |
| first_indexed | 2024-03-12T05:15:36Z |
| format | Article |
| id | doaj.art-679b177d15c04872bf378fb2f366e293 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T05:15:36Z |
| publishDate | 2022-05-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-679b177d15c04872bf378fb2f366e2932023-09-03T08:09:15ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2022-05-014066824825510.48305/jims.v40.i668.024817205Evaluation of Rapid Diagnosis of Candidemia by Agglutination Test Using Gold NanoparticlesZainab Mussaie0Hossein Yousofi-Darani1Hossein Hashemi2Iman Zareie3Parvin Dehghan4MSc Student of Medical Mycology, Department of Mycology and Parasitology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranProfessor, Department of Mycology and Parasitology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranPhD in Medical Parasitology, Shahrekord Blood Transfusion Organization, Shahrekord, Shahrekord, IranMSc student of Medical Mycology, Mycology and Parasitology Department, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssociate Professor, Department of Mycology and Parasitology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranBackground: Candidemia showed high morbidity and mortality and should be diagnosed immediately. The aim of this study was to provide a simple and rapid method for direct detection of Candida in the blood, using conjugated antibodies with gold nanoparticles by the agglutination method.Methods: In this study, yeast species isolated from 40 blood samples of patients tested by the BACTEC method were used. After identifying the etiologic agents by morphological and molecular methods, a mixture of yeast antigens was prepared. Briefly, a mixture of antigens was prepared from the Candida isolates detected from candidemia, and injected into healthy rabbit skin with complete adjuvant to produce antibodies. After injecting the incomplete adjuvant in four reminders, blood samples and serum were finally prepared from the rabbit. Antibody production and its efficacy were confirmed by ELISA method. Gold nanoparticles were chemically conjugated with antibodies. For rapid diagnosis of candidemia, the nanoparticle method was examined on the blood of four patients and blood contaminated with a certain number of Candida yeasts in the laboratory.Findings: Candida albicans was the most common etiologic agent of candidemia, followed by Candida glabrata and Candida parapsilosis complex. In determining the cut-off nanoparticle method, the least yeast detectable in the patient's blood was one yeast. The sensitivity and specificity of the nanoparticle method for detecting Candida in the blood of four patients compared to blood culture was 100%.Conclusion: Under our laboratory conditions, if testing 15 µl blood of a suspected patient to candidemia containing one yeast or even just Candida antigens exposed to 35 microliters of the nanoparticles conjugated antibodies, it shows agglutination during one minute.https://jims.mui.ac.ir/article_17205_2b343ac0945c5d3072b2bfa91ed0420a.pdfcandidemiananoparticlesagglutinationantibody |
| spellingShingle | Zainab Mussaie Hossein Yousofi-Darani Hossein Hashemi Iman Zareie Parvin Dehghan Evaluation of Rapid Diagnosis of Candidemia by Agglutination Test Using Gold Nanoparticles مجله دانشکده پزشکی اصفهان candidemia nanoparticles agglutination antibody |
| title | Evaluation of Rapid Diagnosis of Candidemia by Agglutination Test Using Gold Nanoparticles |
| title_full | Evaluation of Rapid Diagnosis of Candidemia by Agglutination Test Using Gold Nanoparticles |
| title_fullStr | Evaluation of Rapid Diagnosis of Candidemia by Agglutination Test Using Gold Nanoparticles |
| title_full_unstemmed | Evaluation of Rapid Diagnosis of Candidemia by Agglutination Test Using Gold Nanoparticles |
| title_short | Evaluation of Rapid Diagnosis of Candidemia by Agglutination Test Using Gold Nanoparticles |
| title_sort | evaluation of rapid diagnosis of candidemia by agglutination test using gold nanoparticles |
| topic | candidemia nanoparticles agglutination antibody |
| url | https://jims.mui.ac.ir/article_17205_2b343ac0945c5d3072b2bfa91ed0420a.pdf |
| work_keys_str_mv | AT zainabmussaie evaluationofrapiddiagnosisofcandidemiabyagglutinationtestusinggoldnanoparticles AT hosseinyousofidarani evaluationofrapiddiagnosisofcandidemiabyagglutinationtestusinggoldnanoparticles AT hosseinhashemi evaluationofrapiddiagnosisofcandidemiabyagglutinationtestusinggoldnanoparticles AT imanzareie evaluationofrapiddiagnosisofcandidemiabyagglutinationtestusinggoldnanoparticles AT parvindehghan evaluationofrapiddiagnosisofcandidemiabyagglutinationtestusinggoldnanoparticles |