Comparison of In Vitro Bacterial Susceptibility to Common and Novel Equine Wound Care Dressings
Antimicrobial resistance is becoming a problem of concern in the veterinary field, necessitating the use of effective topical treatments to aid the healing of wounds. Honey has been used for thousands of years for its medicinal properties, but in recent years medical-grade Manuka honey has been used...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/14/5/776 |
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| author | Merrill Simpson Dean A. Hendrickson Doreene R. Hyatt Sangeeta Rao |
| author_facet | Merrill Simpson Dean A. Hendrickson Doreene R. Hyatt Sangeeta Rao |
| author_sort | Merrill Simpson |
| collection | DOAJ |
| description | Antimicrobial resistance is becoming a problem of concern in the veterinary field, necessitating the use of effective topical treatments to aid the healing of wounds. Honey has been used for thousands of years for its medicinal properties, but in recent years medical-grade Manuka honey has been used to treat infected wounds. The goal of this study was to determine the relative susceptibility of four common equine wound pathogens to ten different types of antimicrobial agents based on the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The pathogens studied include ATCC lab-acclimated <i>Pseudomonas aeruginosa</i>, <i>Escherichia coli</i>, and methicillin-resistant <i>Staphylococcus aureus</i> and one from an equine sample submitted to the Colorado State Veterinary Diagnostic Laboratory (<i>Streptococcus equi</i> ssp. <i>zooepidemicus</i> (<i>Streptococcus zooepidemicus</i>)). An additional goal of the study was to describe the comparison of bactericidal activity of medical-grade Manuka honey, local honey, and commercial, food-grade honey to other commonly used wound dressings (20% hypertonic saline, silver sulfadiazine cream, PHMB gauze, and PHMB foam). The objective is to provide veterinary practitioners with comparative data on the use of a variety of antimicrobial dressings for inhibiting the growth of common wound bacteria. MIC and MBC for Manuka, store, and local honeys were comparable to those of sterile gauze, sugar, and hypertonic saline. Across bacterial species, local honey proved to have more bactericidal activity when compared to Manuka honey and commercial, food-grade honey. The MIC and MBC for PHMB gauze and foam was consistently at a higher dilution compared to the other antimicrobials. The majority of antimicrobials exhibited stronger inhibitory and bactericidal activity against a Streptococcus zooepidemicus isolate obtained from a wound compared to other bacteria that were ATCC lab-acclimated. Additional research for in vivo applications needs to be done to see whether differences exist in effective wound management. |
| first_indexed | 2024-04-25T00:36:31Z |
| format | Article |
| id | doaj.art-679b4cbc3df14e759003b9c5b5b159a3 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-04-25T00:36:31Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-679b4cbc3df14e759003b9c5b5b159a32024-03-12T16:38:17ZengMDPI AGAnimals2076-26152024-03-0114577610.3390/ani14050776Comparison of In Vitro Bacterial Susceptibility to Common and Novel Equine Wound Care DressingsMerrill Simpson0Dean A. Hendrickson1Doreene R. Hyatt2Sangeeta Rao3Department of Clinical Sciences, Colorado State University, Fort Collins, CO 80523, USADepartment of Clinical Sciences, Colorado State University, Fort Collins, CO 80523, USADepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USADepartment of Clinical Sciences, Colorado State University, Fort Collins, CO 80523, USAAntimicrobial resistance is becoming a problem of concern in the veterinary field, necessitating the use of effective topical treatments to aid the healing of wounds. Honey has been used for thousands of years for its medicinal properties, but in recent years medical-grade Manuka honey has been used to treat infected wounds. The goal of this study was to determine the relative susceptibility of four common equine wound pathogens to ten different types of antimicrobial agents based on the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The pathogens studied include ATCC lab-acclimated <i>Pseudomonas aeruginosa</i>, <i>Escherichia coli</i>, and methicillin-resistant <i>Staphylococcus aureus</i> and one from an equine sample submitted to the Colorado State Veterinary Diagnostic Laboratory (<i>Streptococcus equi</i> ssp. <i>zooepidemicus</i> (<i>Streptococcus zooepidemicus</i>)). An additional goal of the study was to describe the comparison of bactericidal activity of medical-grade Manuka honey, local honey, and commercial, food-grade honey to other commonly used wound dressings (20% hypertonic saline, silver sulfadiazine cream, PHMB gauze, and PHMB foam). The objective is to provide veterinary practitioners with comparative data on the use of a variety of antimicrobial dressings for inhibiting the growth of common wound bacteria. MIC and MBC for Manuka, store, and local honeys were comparable to those of sterile gauze, sugar, and hypertonic saline. Across bacterial species, local honey proved to have more bactericidal activity when compared to Manuka honey and commercial, food-grade honey. The MIC and MBC for PHMB gauze and foam was consistently at a higher dilution compared to the other antimicrobials. The majority of antimicrobials exhibited stronger inhibitory and bactericidal activity against a Streptococcus zooepidemicus isolate obtained from a wound compared to other bacteria that were ATCC lab-acclimated. Additional research for in vivo applications needs to be done to see whether differences exist in effective wound management.https://www.mdpi.com/2076-2615/14/5/776wound dressingbacterial susceptibilityminimum inhibitory concentration (MIC)minimum bactericidal concentration (MBC)Manuka honeyhypertonic saline |
| spellingShingle | Merrill Simpson Dean A. Hendrickson Doreene R. Hyatt Sangeeta Rao Comparison of In Vitro Bacterial Susceptibility to Common and Novel Equine Wound Care Dressings Animals wound dressing bacterial susceptibility minimum inhibitory concentration (MIC) minimum bactericidal concentration (MBC) Manuka honey hypertonic saline |
| title | Comparison of In Vitro Bacterial Susceptibility to Common and Novel Equine Wound Care Dressings |
| title_full | Comparison of In Vitro Bacterial Susceptibility to Common and Novel Equine Wound Care Dressings |
| title_fullStr | Comparison of In Vitro Bacterial Susceptibility to Common and Novel Equine Wound Care Dressings |
| title_full_unstemmed | Comparison of In Vitro Bacterial Susceptibility to Common and Novel Equine Wound Care Dressings |
| title_short | Comparison of In Vitro Bacterial Susceptibility to Common and Novel Equine Wound Care Dressings |
| title_sort | comparison of in vitro bacterial susceptibility to common and novel equine wound care dressings |
| topic | wound dressing bacterial susceptibility minimum inhibitory concentration (MIC) minimum bactericidal concentration (MBC) Manuka honey hypertonic saline |
| url | https://www.mdpi.com/2076-2615/14/5/776 |
| work_keys_str_mv | AT merrillsimpson comparisonofinvitrobacterialsusceptibilitytocommonandnovelequinewoundcaredressings AT deanahendrickson comparisonofinvitrobacterialsusceptibilitytocommonandnovelequinewoundcaredressings AT doreenerhyatt comparisonofinvitrobacterialsusceptibilitytocommonandnovelequinewoundcaredressings AT sangeetarao comparisonofinvitrobacterialsusceptibilitytocommonandnovelequinewoundcaredressings |