Adipocyte hypertrophy associates with in vivo postprandial fatty acid metabolism and adipose single-cell transcriptional dynamics
Summary: Adipocyte hypertrophy is associated with metabolic complications independent of obesity. We aimed to determine: 1) the association between adipocyte size and postprandial fatty acid metabolism; 2) the potential mechanisms driving the obesity-independent, hypertrophy-associated dysmetabolism...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223027694 |
| _version_ | 1827400237585530880 |
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| author | Run Zhou Ye Emilie Montastier Frédérique Frisch Christophe Noll Hugues Allard-Chamard Nicolas Gévry André Tchernof André C. Carpentier |
| author_facet | Run Zhou Ye Emilie Montastier Frédérique Frisch Christophe Noll Hugues Allard-Chamard Nicolas Gévry André Tchernof André C. Carpentier |
| author_sort | Run Zhou Ye |
| collection | DOAJ |
| description | Summary: Adipocyte hypertrophy is associated with metabolic complications independent of obesity. We aimed to determine: 1) the association between adipocyte size and postprandial fatty acid metabolism; 2) the potential mechanisms driving the obesity-independent, hypertrophy-associated dysmetabolism in vivo and at a single-cell resolution. Tracers with positron emission tomography were used to measure fatty acid metabolism in 40 men and women with normal or impaired glucose tolerance (NCT02808182), and single nuclei RNA-sequencing (snRNA-seq) to determine transcriptional dynamics of subcutaneous adipose tissue (AT) between individuals with AT hypertrophy vs. hyperplasia matched for sex, ethnicity, glucose-tolerance status, BMI, total and percent body fat, and waist circumference. Adipocyte size was associated with high postprandial total cardiac fatty acid uptake and higher visceral AT dietary fatty acid uptake, but lower lean tissue dietary fatty acid uptake. We found major shifts in cell transcriptomal dynamics with AT hypertrophy that were consistent with in vivo metabolic changes. |
| first_indexed | 2024-03-08T19:58:08Z |
| format | Article |
| id | doaj.art-679ccead7b3043cbb1c81f76245c3683 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-08T19:58:08Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-679ccead7b3043cbb1c81f76245c36832023-12-24T04:46:18ZengElsevieriScience2589-00422024-01-01271108692Adipocyte hypertrophy associates with in vivo postprandial fatty acid metabolism and adipose single-cell transcriptional dynamicsRun Zhou Ye0Emilie Montastier1Frédérique Frisch2Christophe Noll3Hugues Allard-Chamard4Nicolas Gévry5André Tchernof6André C. Carpentier7Division of Endocrinology, Department of Medicine, Centre de recherche du CHUS, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, CanadaDivision of Endocrinology, Department of Medicine, Centre de recherche du CHUS, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, CanadaDivision of Endocrinology, Department of Medicine, Centre de recherche du CHUS, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, CanadaDivision of Endocrinology, Department of Medicine, Centre de recherche du CHUS, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, CanadaDivision of Endocrinology, Department of Medicine, Centre de recherche du CHUS, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, CanadaDepartment of Biology, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, CanadaQuébec Heart and Lung Research Institute, Laval University, Québec, QC G1V 4G5, CanadaDivision of Endocrinology, Department of Medicine, Centre de recherche du CHUS, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada; Department of Nuclear Medicine and Radiobiology, Centre de Recherche du CHUS, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada; Corresponding authorSummary: Adipocyte hypertrophy is associated with metabolic complications independent of obesity. We aimed to determine: 1) the association between adipocyte size and postprandial fatty acid metabolism; 2) the potential mechanisms driving the obesity-independent, hypertrophy-associated dysmetabolism in vivo and at a single-cell resolution. Tracers with positron emission tomography were used to measure fatty acid metabolism in 40 men and women with normal or impaired glucose tolerance (NCT02808182), and single nuclei RNA-sequencing (snRNA-seq) to determine transcriptional dynamics of subcutaneous adipose tissue (AT) between individuals with AT hypertrophy vs. hyperplasia matched for sex, ethnicity, glucose-tolerance status, BMI, total and percent body fat, and waist circumference. Adipocyte size was associated with high postprandial total cardiac fatty acid uptake and higher visceral AT dietary fatty acid uptake, but lower lean tissue dietary fatty acid uptake. We found major shifts in cell transcriptomal dynamics with AT hypertrophy that were consistent with in vivo metabolic changes.http://www.sciencedirect.com/science/article/pii/S2589004223027694Human metabolismCell biologyTranscriptomics |
| spellingShingle | Run Zhou Ye Emilie Montastier Frédérique Frisch Christophe Noll Hugues Allard-Chamard Nicolas Gévry André Tchernof André C. Carpentier Adipocyte hypertrophy associates with in vivo postprandial fatty acid metabolism and adipose single-cell transcriptional dynamics iScience Human metabolism Cell biology Transcriptomics |
| title | Adipocyte hypertrophy associates with in vivo postprandial fatty acid metabolism and adipose single-cell transcriptional dynamics |
| title_full | Adipocyte hypertrophy associates with in vivo postprandial fatty acid metabolism and adipose single-cell transcriptional dynamics |
| title_fullStr | Adipocyte hypertrophy associates with in vivo postprandial fatty acid metabolism and adipose single-cell transcriptional dynamics |
| title_full_unstemmed | Adipocyte hypertrophy associates with in vivo postprandial fatty acid metabolism and adipose single-cell transcriptional dynamics |
| title_short | Adipocyte hypertrophy associates with in vivo postprandial fatty acid metabolism and adipose single-cell transcriptional dynamics |
| title_sort | adipocyte hypertrophy associates with in vivo postprandial fatty acid metabolism and adipose single cell transcriptional dynamics |
| topic | Human metabolism Cell biology Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223027694 |
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