Detection and Aggregation of Listeria Monocytogenes Using Polyclonal Antibody Gold-Coated Magnetic Nanoshells Surface-Enhanced Raman Spectroscopy Substrates
Magnetic nanoshells with tailored surface chemistry can enhance bacterial detection and separation technologies. This work demonstrated a simple technique to detect, capture, and aggregate bacteria with the aid of end-functionalized polyclonal antibody gold-coated magnetic nanoshells (pAb-Lis-AuMNs)...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Nanotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnano.2021.653744/full |
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| author | Robert T. Busch Farzia Karim Yvonne Sun Yvonne Sun H. Christopher Fry Yuzi Liu Chenglong Zhao Chenglong Zhao Erick S. Vasquez Erick S. Vasquez |
| author_facet | Robert T. Busch Farzia Karim Yvonne Sun Yvonne Sun H. Christopher Fry Yuzi Liu Chenglong Zhao Chenglong Zhao Erick S. Vasquez Erick S. Vasquez |
| author_sort | Robert T. Busch |
| collection | DOAJ |
| description | Magnetic nanoshells with tailored surface chemistry can enhance bacterial detection and separation technologies. This work demonstrated a simple technique to detect, capture, and aggregate bacteria with the aid of end-functionalized polyclonal antibody gold-coated magnetic nanoshells (pAb-Lis-AuMNs) as surface-enhanced Raman spectroscopy (SERS) probes. Listeria monocytogenes were used as the pathogenic bacteria and the pAb-Lis-AuMNs, 300 nm diameter, were used as probes allowing facile magnetic separation and aggregation. An optimized covalent bioconjugation procedure between the magnetic nanoshells and the polyclonal antibody was performed at pH six via a carbodiimide crosslinking reaction. Spectroscopic and morphological characterization techniques confirmed the fabrication of stable pAb-Lis-AuMNs. The resulting pAb-Lis-AuMNs acted as a SERS probe for L. monocytogenes based on the targeted capture via surface binding interactions and magnetically induced aggregation. Label-free SERS measurements were recorded for the minimum detectable amount of L. monocytogenes based on the SERS intensity at the 1388 cm−1 Raman shift. L. monocytogenes concentrations exhibited detection limits in the range of 104–107 CFU ml−1, before and after aggregation. By fitting these concentrations, the limit of detection of this method was ∼103 CFU ml−1. Using a low-intensity magnetic field of 35 G, pAb-Lis-AuMNs aggregated L. monocytogenes as demonstrated with microscopy techniques, including SEM and optical microscopy. Overall, this work presents a label-free SERS probe method comprised of a surface-modified polyclonal antibody sub-micron magnetic nanoshell structures with high sensitivity and magnetic induced separation that could lead to the fabrication of multiple single-step sensors. |
| first_indexed | 2024-12-20T01:22:22Z |
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| id | doaj.art-679da3cf5c4548c88fb5c6ae780a2219 |
| institution | Directory Open Access Journal |
| issn | 2673-3013 |
| language | English |
| last_indexed | 2024-12-20T01:22:22Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nanotechnology |
| spelling | doaj.art-679da3cf5c4548c88fb5c6ae780a22192022-12-21T19:58:22ZengFrontiers Media S.A.Frontiers in Nanotechnology2673-30132021-04-01310.3389/fnano.2021.653744653744Detection and Aggregation of Listeria Monocytogenes Using Polyclonal Antibody Gold-Coated Magnetic Nanoshells Surface-Enhanced Raman Spectroscopy SubstratesRobert T. Busch0Farzia Karim1Yvonne Sun2Yvonne Sun3H. Christopher Fry4Yuzi Liu5Chenglong Zhao6Chenglong Zhao7Erick S. Vasquez8Erick S. Vasquez9Department of Chemical and Materials Engineering, University of Dayton, Dayton, OH, United StatesDepartment of Electro-Optics and Photonics, University of Dayton, Dayton, OH, United StatesDepartment of Biology, University of Dayton, Dayton, OH, United StatesIntegrative Science and Engineering Center, University of Dayton, Dayton, OH, United StatesCenter for Nanoscale Materials, Argonne National Laboratory, Lemont, IL, United StatesCenter for Nanoscale Materials, Argonne National Laboratory, Lemont, IL, United StatesDepartment of Electro-Optics and Photonics, University of Dayton, Dayton, OH, United StatesDepartment of Physics, University of Dayton, Dayton, OH, United StatesDepartment of Chemical and Materials Engineering, University of Dayton, Dayton, OH, United StatesIntegrative Science and Engineering Center, University of Dayton, Dayton, OH, United StatesMagnetic nanoshells with tailored surface chemistry can enhance bacterial detection and separation technologies. This work demonstrated a simple technique to detect, capture, and aggregate bacteria with the aid of end-functionalized polyclonal antibody gold-coated magnetic nanoshells (pAb-Lis-AuMNs) as surface-enhanced Raman spectroscopy (SERS) probes. Listeria monocytogenes were used as the pathogenic bacteria and the pAb-Lis-AuMNs, 300 nm diameter, were used as probes allowing facile magnetic separation and aggregation. An optimized covalent bioconjugation procedure between the magnetic nanoshells and the polyclonal antibody was performed at pH six via a carbodiimide crosslinking reaction. Spectroscopic and morphological characterization techniques confirmed the fabrication of stable pAb-Lis-AuMNs. The resulting pAb-Lis-AuMNs acted as a SERS probe for L. monocytogenes based on the targeted capture via surface binding interactions and magnetically induced aggregation. Label-free SERS measurements were recorded for the minimum detectable amount of L. monocytogenes based on the SERS intensity at the 1388 cm−1 Raman shift. L. monocytogenes concentrations exhibited detection limits in the range of 104–107 CFU ml−1, before and after aggregation. By fitting these concentrations, the limit of detection of this method was ∼103 CFU ml−1. Using a low-intensity magnetic field of 35 G, pAb-Lis-AuMNs aggregated L. monocytogenes as demonstrated with microscopy techniques, including SEM and optical microscopy. Overall, this work presents a label-free SERS probe method comprised of a surface-modified polyclonal antibody sub-micron magnetic nanoshell structures with high sensitivity and magnetic induced separation that could lead to the fabrication of multiple single-step sensors.https://www.frontiersin.org/articles/10.3389/fnano.2021.653744/fulllisteria monocytogenespolyclonal antibodygold-coated magnetic nanoshellsSERS detectionmagnetic separationsurface functionalization |
| spellingShingle | Robert T. Busch Farzia Karim Yvonne Sun Yvonne Sun H. Christopher Fry Yuzi Liu Chenglong Zhao Chenglong Zhao Erick S. Vasquez Erick S. Vasquez Detection and Aggregation of Listeria Monocytogenes Using Polyclonal Antibody Gold-Coated Magnetic Nanoshells Surface-Enhanced Raman Spectroscopy Substrates Frontiers in Nanotechnology listeria monocytogenes polyclonal antibody gold-coated magnetic nanoshells SERS detection magnetic separation surface functionalization |
| title | Detection and Aggregation of Listeria Monocytogenes Using Polyclonal Antibody Gold-Coated Magnetic Nanoshells Surface-Enhanced Raman Spectroscopy Substrates |
| title_full | Detection and Aggregation of Listeria Monocytogenes Using Polyclonal Antibody Gold-Coated Magnetic Nanoshells Surface-Enhanced Raman Spectroscopy Substrates |
| title_fullStr | Detection and Aggregation of Listeria Monocytogenes Using Polyclonal Antibody Gold-Coated Magnetic Nanoshells Surface-Enhanced Raman Spectroscopy Substrates |
| title_full_unstemmed | Detection and Aggregation of Listeria Monocytogenes Using Polyclonal Antibody Gold-Coated Magnetic Nanoshells Surface-Enhanced Raman Spectroscopy Substrates |
| title_short | Detection and Aggregation of Listeria Monocytogenes Using Polyclonal Antibody Gold-Coated Magnetic Nanoshells Surface-Enhanced Raman Spectroscopy Substrates |
| title_sort | detection and aggregation of listeria monocytogenes using polyclonal antibody gold coated magnetic nanoshells surface enhanced raman spectroscopy substrates |
| topic | listeria monocytogenes polyclonal antibody gold-coated magnetic nanoshells SERS detection magnetic separation surface functionalization |
| url | https://www.frontiersin.org/articles/10.3389/fnano.2021.653744/full |
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