MODIFICATION VARIABILITY OF AURICULARIA AURICULA-JUDAE, CULTURED ON MEDIA OF DIFFERENT COMPOSITIONS
Aim. Defining features of modification variability of A. auricula-judae vegetative mycelium depending on the composition of the nutrient medium at the coefficient of growth, colony morphology and multiple molecular forms of carboxylesterases. Methods. Vegetative mycelium of Auricularia auricula-juda...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Odessa I. I. Mechnikov National University
2014-06-01
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| Series: | Mìkrobìologìâ ì Bìotehnologìâ |
| Subjects: | |
| Online Access: | http://mbt.onu.edu.ua/article/view/48260 |
| Summary: | Aim. Defining features of modification variability of A. auricula-judae vegetative mycelium depending on the composition of the nutrient medium at the coefficient of growth, colony morphology and multiple molecular forms of carboxylesterases. Methods. Vegetative mycelium of Auricularia auricula-judae (Bull.: Fr.) Quél. ONU F201 strain was grown in the following environments: wort agar (CA) – control variant, wheat agar (ПA), oatmeal agar (BA), barley agar (ЯA), buckwheat agar (ГA), millet agar (ПрА), potato and glucose agar with high glucose content (KГA 10%). The modified coefficient of growth (PKj) and morphology of the obtained colonies were determined. Using electrophoretic separation on 7% polyacrylamide gels the number of multiple molecular forms (MMF) of carboxylesterases in mycelium of each option were established. Ste statistical analysis of the data was performed using non-parametric Wilcoxon test. The growth character of the colonies ranged from dense cottony, fast growing to arachnoid, slow growing; the number of isoformes of the enzyme varied from 2 to 10, depending on the composition of the substrate. The rank order of the number of isoformes did not match the rank of the coefficient of growth of mycelium. Conclusions. Vegetative mycelium of A. auricula-judae has extensive modification variability cultured on different media, both morphological and biochemical characteristics. Depending on the composition of the nutrient medium in the mycelium A. auricula-judae expressed from 2 to 10 isoforms of carboxylesterases, only two of which – with Rf 0.50 and 0.54, were determined in all the variants of studied culture media. |
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| ISSN: | 2076-0558 2307-4663 |