Identification of miRNAs Involving Potato-<i>Phytophthora infestans</i> Interaction
sRNAs (small RNAs) play an important role in regulation of plant immunity against a variety of pathogens. In this study, sRNA sequencing analysis was performed to identify miRNAs (microRNAs) during the interaction of potato and <i>Phytophthora infestans</i>. Totally, 171 potato miRNAs we...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/3/461 |
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| author | Ming Luo Xinyuan Sun Meng Xu Zhendong Tian |
| author_facet | Ming Luo Xinyuan Sun Meng Xu Zhendong Tian |
| author_sort | Ming Luo |
| collection | DOAJ |
| description | sRNAs (small RNAs) play an important role in regulation of plant immunity against a variety of pathogens. In this study, sRNA sequencing analysis was performed to identify miRNAs (microRNAs) during the interaction of potato and <i>Phytophthora infestans</i>. Totally, 171 potato miRNAs were identified, 43 of which were annotated in the miRNA database and 128 were assigned as novel miRNAs in this study. Those potato miRNAs may target 878 potato genes and half of them encode resistance proteins. Fifty-three potato miRNAs may target 194 <i>P. infestans</i> genes. Three potato miRNAs (novel 72, 133, and 140) were predicted to have targets only in the <i>P. infestans</i> genome. miRNAs transient expression and <i>P. infestans</i> inoculation assay showed that miR396, miR166, miR6149-5P, novel133, or novel140 promoted <i>P. infestans</i> colonization, while miR394 inhibited colonization on <i>Nicotiana benthamiana</i> leaves. An artificial miRNA target (amiRNA) degradation experiment demonstrated that miR394 could target both potato gene (<i>PGSC0003DMG400034305</i>) and <i>P. infestans</i> genes. miR396 targets the multicystatin gene (<i>PGSC0003DMG400026899</i>) and miR6149-5p could shear the galactose oxidase F-box protein gene <i>CPR30</i> (<i>PGSC0003DMG400021641</i>). This study provides new information on the aspect of cross-kingdom immune regulation in potato-<i>P. infestans</i> interaction at the sRNAs regulation level. |
| first_indexed | 2024-03-11T09:30:26Z |
| format | Article |
| id | doaj.art-67a2bde8131d4898bcde821a46c099d0 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T09:30:26Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-67a2bde8131d4898bcde821a46c099d02023-11-16T17:42:40ZengMDPI AGPlants2223-77472023-01-0112346110.3390/plants12030461Identification of miRNAs Involving Potato-<i>Phytophthora infestans</i> InteractionMing Luo0Xinyuan Sun1Meng Xu2Zhendong Tian3National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University (HZAU), Wuhan 430070, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University (HZAU), Wuhan 430070, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University (HZAU), Wuhan 430070, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University (HZAU), Wuhan 430070, ChinasRNAs (small RNAs) play an important role in regulation of plant immunity against a variety of pathogens. In this study, sRNA sequencing analysis was performed to identify miRNAs (microRNAs) during the interaction of potato and <i>Phytophthora infestans</i>. Totally, 171 potato miRNAs were identified, 43 of which were annotated in the miRNA database and 128 were assigned as novel miRNAs in this study. Those potato miRNAs may target 878 potato genes and half of them encode resistance proteins. Fifty-three potato miRNAs may target 194 <i>P. infestans</i> genes. Three potato miRNAs (novel 72, 133, and 140) were predicted to have targets only in the <i>P. infestans</i> genome. miRNAs transient expression and <i>P. infestans</i> inoculation assay showed that miR396, miR166, miR6149-5P, novel133, or novel140 promoted <i>P. infestans</i> colonization, while miR394 inhibited colonization on <i>Nicotiana benthamiana</i> leaves. An artificial miRNA target (amiRNA) degradation experiment demonstrated that miR394 could target both potato gene (<i>PGSC0003DMG400034305</i>) and <i>P. infestans</i> genes. miR396 targets the multicystatin gene (<i>PGSC0003DMG400026899</i>) and miR6149-5p could shear the galactose oxidase F-box protein gene <i>CPR30</i> (<i>PGSC0003DMG400021641</i>). This study provides new information on the aspect of cross-kingdom immune regulation in potato-<i>P. infestans</i> interaction at the sRNAs regulation level.https://www.mdpi.com/2223-7747/12/3/461potato<i>Phytophthora infestans</i>miRNAdisease resistancetransboundary regulation |
| spellingShingle | Ming Luo Xinyuan Sun Meng Xu Zhendong Tian Identification of miRNAs Involving Potato-<i>Phytophthora infestans</i> Interaction Plants potato <i>Phytophthora infestans</i> miRNA disease resistance transboundary regulation |
| title | Identification of miRNAs Involving Potato-<i>Phytophthora infestans</i> Interaction |
| title_full | Identification of miRNAs Involving Potato-<i>Phytophthora infestans</i> Interaction |
| title_fullStr | Identification of miRNAs Involving Potato-<i>Phytophthora infestans</i> Interaction |
| title_full_unstemmed | Identification of miRNAs Involving Potato-<i>Phytophthora infestans</i> Interaction |
| title_short | Identification of miRNAs Involving Potato-<i>Phytophthora infestans</i> Interaction |
| title_sort | identification of mirnas involving potato i phytophthora infestans i interaction |
| topic | potato <i>Phytophthora infestans</i> miRNA disease resistance transboundary regulation |
| url | https://www.mdpi.com/2223-7747/12/3/461 |
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