Carrageenan-Based Crowding and Confinement Combination Approach to Increase Collagen Deposition for In Vitro Tissue Development
Connective tissue models grown from cell monolayers can be instrumental in a variety of biomedical fields such as drug screening, wound healing, and regenerative engineering. However, while connective tissues contain abundant fibrillar collagen, achieving a sufficient assembly and retention of fibri...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/9/9/705 |
| _version_ | 1797579952747446272 |
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| author | Joseph Krebs Samuel Stealey Alyssa Brown Austin Krohn Silviya Petrova Zustiak Natasha Case |
| author_facet | Joseph Krebs Samuel Stealey Alyssa Brown Austin Krohn Silviya Petrova Zustiak Natasha Case |
| author_sort | Joseph Krebs |
| collection | DOAJ |
| description | Connective tissue models grown from cell monolayers can be instrumental in a variety of biomedical fields such as drug screening, wound healing, and regenerative engineering. However, while connective tissues contain abundant fibrillar collagen, achieving a sufficient assembly and retention of fibrillar collagen in vitro is challenging. Unlike the dilute cell culture environment, the body’s environment is characterized by a high density of soluble macromolecules (crowding) and macromolecular networks (confinement), which contribute to extracellular matrix (ECM) assembly in vivo. Consequently, macromolecular crowding (MMC) has been successfully used to enhance the processing of type I procollagen, leading to significant increases in fibrillar collagen assembly and accumulation during in vitro culture of a variety of cell types. In this study, we developed a combination approach using a carrageenan hydrogel, which released soluble macromolecules and served as a confinement barrier. We first evaluated the local carrageenan release and then confirmed the effectiveness of this combination approach on collagen accumulation by the human MG-63 bone cell line. Additionally, computational modeling of oxygen and glucose transport within the culture system showed no negative effects of the hydrogel and its releasates on cell viability. |
| first_indexed | 2024-03-10T22:44:20Z |
| format | Article |
| id | doaj.art-67a32a436810420e9df920fba6035678 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-10T22:44:20Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-67a32a436810420e9df920fba60356782023-11-19T10:50:39ZengMDPI AGGels2310-28612023-09-019970510.3390/gels9090705Carrageenan-Based Crowding and Confinement Combination Approach to Increase Collagen Deposition for In Vitro Tissue DevelopmentJoseph Krebs0Samuel Stealey1Alyssa Brown2Austin Krohn3Silviya Petrova Zustiak4Natasha Case5Department of Biomedical Engineering, Saint Louis University, Saint Louis, MO 63103, USADepartment of Biomedical Engineering, Saint Louis University, Saint Louis, MO 63103, USADepartment of Biomedical Engineering, Saint Louis University, Saint Louis, MO 63103, USADepartment of Biomedical Engineering, Saint Louis University, Saint Louis, MO 63103, USADepartment of Biomedical Engineering, Saint Louis University, Saint Louis, MO 63103, USADepartment of Biomedical Engineering, Saint Louis University, Saint Louis, MO 63103, USAConnective tissue models grown from cell monolayers can be instrumental in a variety of biomedical fields such as drug screening, wound healing, and regenerative engineering. However, while connective tissues contain abundant fibrillar collagen, achieving a sufficient assembly and retention of fibrillar collagen in vitro is challenging. Unlike the dilute cell culture environment, the body’s environment is characterized by a high density of soluble macromolecules (crowding) and macromolecular networks (confinement), which contribute to extracellular matrix (ECM) assembly in vivo. Consequently, macromolecular crowding (MMC) has been successfully used to enhance the processing of type I procollagen, leading to significant increases in fibrillar collagen assembly and accumulation during in vitro culture of a variety of cell types. In this study, we developed a combination approach using a carrageenan hydrogel, which released soluble macromolecules and served as a confinement barrier. We first evaluated the local carrageenan release and then confirmed the effectiveness of this combination approach on collagen accumulation by the human MG-63 bone cell line. Additionally, computational modeling of oxygen and glucose transport within the culture system showed no negative effects of the hydrogel and its releasates on cell viability.https://www.mdpi.com/2310-2861/9/9/705macromolecular crowdinghydrogelcollagenextracellular matrix synthesis |
| spellingShingle | Joseph Krebs Samuel Stealey Alyssa Brown Austin Krohn Silviya Petrova Zustiak Natasha Case Carrageenan-Based Crowding and Confinement Combination Approach to Increase Collagen Deposition for In Vitro Tissue Development Gels macromolecular crowding hydrogel collagen extracellular matrix synthesis |
| title | Carrageenan-Based Crowding and Confinement Combination Approach to Increase Collagen Deposition for In Vitro Tissue Development |
| title_full | Carrageenan-Based Crowding and Confinement Combination Approach to Increase Collagen Deposition for In Vitro Tissue Development |
| title_fullStr | Carrageenan-Based Crowding and Confinement Combination Approach to Increase Collagen Deposition for In Vitro Tissue Development |
| title_full_unstemmed | Carrageenan-Based Crowding and Confinement Combination Approach to Increase Collagen Deposition for In Vitro Tissue Development |
| title_short | Carrageenan-Based Crowding and Confinement Combination Approach to Increase Collagen Deposition for In Vitro Tissue Development |
| title_sort | carrageenan based crowding and confinement combination approach to increase collagen deposition for in vitro tissue development |
| topic | macromolecular crowding hydrogel collagen extracellular matrix synthesis |
| url | https://www.mdpi.com/2310-2861/9/9/705 |
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