Emergence of Ciprofloxacin-Resistant Salmonella enterica Serovar Typhi in Italy.
In developed countries, typhoid fever is often associated with persons who travel to endemic areas or immigrate from them. Typhoid fever is a systemic infection caused by Salmonella enterica serovar Typhi. Because of the emergence of antimicrobial resistance to standard first-line drugs, fluoroquino...
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| Format: | Article |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4488240?pdf=render |
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| author | Aurora García-Fernández Silvia Gallina Slawomir Owczarek Anna Maria Dionisi Ildo Benedetti Lucia Decastelli Ida Luzzi |
| author_facet | Aurora García-Fernández Silvia Gallina Slawomir Owczarek Anna Maria Dionisi Ildo Benedetti Lucia Decastelli Ida Luzzi |
| author_sort | Aurora García-Fernández |
| collection | DOAJ |
| description | In developed countries, typhoid fever is often associated with persons who travel to endemic areas or immigrate from them. Typhoid fever is a systemic infection caused by Salmonella enterica serovar Typhi. Because of the emergence of antimicrobial resistance to standard first-line drugs, fluoroquinolones are the drugs of choice. Resistance to ciprofloxacin by this Salmonella serovar represents an emerging public health issue. Two S. enterica ser. Typhi strains resistant to ciprofloxacin (CIP) were reported to the Italian surveillance system for foodborne and waterborne diseases (EnterNet-Italia) in 2013. The strains were isolated from two Italian tourists upon their arrival from India. A retrospective analysis of 17 other S. enterica ser. Typhi strains isolated in Italy during 2011-2013 was performed to determine their resistance to CIP. For this purpose, we assayed for susceptibility to antimicrobial agents and conducted PCR and nucleotide sequence analyses. Moreover, all strains were typed using pulsed-field gel electrophoresis to evaluate possible clonal relationships. Sixty-eight percent of the S. enterica ser. Typhi strains were resistant to CIP (MICs, 0.125-16 mg/L), and all isolates were negative for determinants of plasmid-mediated quinolone resistance. Analysis of sequences encoding DNA gyrase and topoisomerase IV subunits revealed mutations in gyrA, gyrB, and parC. Thirteen different clonal groups were detected, and the two CIP-resistant strains isolated from the individuals who visited India exhibited the same PFGE pattern. Because of these findings, the emergence of CIP-resistant S. enterica ser. Typhi isolates in Italy deserves attention, and monitoring antibiotic susceptibility is important for efficiently managing cases of typhoid fever. |
| first_indexed | 2024-12-12T07:06:51Z |
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| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T07:06:51Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-67a5f7baa9d84ac2a0161e63fc88dad52022-12-22T00:33:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e013206510.1371/journal.pone.0132065Emergence of Ciprofloxacin-Resistant Salmonella enterica Serovar Typhi in Italy.Aurora García-FernándezSilvia GallinaSlawomir OwczarekAnna Maria DionisiIldo BenedettiLucia DecastelliIda LuzziIn developed countries, typhoid fever is often associated with persons who travel to endemic areas or immigrate from them. Typhoid fever is a systemic infection caused by Salmonella enterica serovar Typhi. Because of the emergence of antimicrobial resistance to standard first-line drugs, fluoroquinolones are the drugs of choice. Resistance to ciprofloxacin by this Salmonella serovar represents an emerging public health issue. Two S. enterica ser. Typhi strains resistant to ciprofloxacin (CIP) were reported to the Italian surveillance system for foodborne and waterborne diseases (EnterNet-Italia) in 2013. The strains were isolated from two Italian tourists upon their arrival from India. A retrospective analysis of 17 other S. enterica ser. Typhi strains isolated in Italy during 2011-2013 was performed to determine their resistance to CIP. For this purpose, we assayed for susceptibility to antimicrobial agents and conducted PCR and nucleotide sequence analyses. Moreover, all strains were typed using pulsed-field gel electrophoresis to evaluate possible clonal relationships. Sixty-eight percent of the S. enterica ser. Typhi strains were resistant to CIP (MICs, 0.125-16 mg/L), and all isolates were negative for determinants of plasmid-mediated quinolone resistance. Analysis of sequences encoding DNA gyrase and topoisomerase IV subunits revealed mutations in gyrA, gyrB, and parC. Thirteen different clonal groups were detected, and the two CIP-resistant strains isolated from the individuals who visited India exhibited the same PFGE pattern. Because of these findings, the emergence of CIP-resistant S. enterica ser. Typhi isolates in Italy deserves attention, and monitoring antibiotic susceptibility is important for efficiently managing cases of typhoid fever.http://europepmc.org/articles/PMC4488240?pdf=render |
| spellingShingle | Aurora García-Fernández Silvia Gallina Slawomir Owczarek Anna Maria Dionisi Ildo Benedetti Lucia Decastelli Ida Luzzi Emergence of Ciprofloxacin-Resistant Salmonella enterica Serovar Typhi in Italy. PLoS ONE |
| title | Emergence of Ciprofloxacin-Resistant Salmonella enterica Serovar Typhi in Italy. |
| title_full | Emergence of Ciprofloxacin-Resistant Salmonella enterica Serovar Typhi in Italy. |
| title_fullStr | Emergence of Ciprofloxacin-Resistant Salmonella enterica Serovar Typhi in Italy. |
| title_full_unstemmed | Emergence of Ciprofloxacin-Resistant Salmonella enterica Serovar Typhi in Italy. |
| title_short | Emergence of Ciprofloxacin-Resistant Salmonella enterica Serovar Typhi in Italy. |
| title_sort | emergence of ciprofloxacin resistant salmonella enterica serovar typhi in italy |
| url | http://europepmc.org/articles/PMC4488240?pdf=render |
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