The Effect of Ampicillin and Gentamicin Conjugated with Gold Nanoparticles on the Formation of Biofilms in Pseudomonas aeruginosa
Background and Objectives: Pseudomonas aeruginosa is a gram-negative bacterium and an opportunistic pathogen with several virulence factors. This bacterium is one of the common pathogens in nosocomial infections, particularly in patients with cystic fibrosis . Also, one of the important mechanisms o...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Qom University of Medical Sciences
2015-07-01
|
| Series: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
| Subjects: | |
| Online Access: | http://journal.muq.ac.ir/article-1-218-en.html |
| _version_ | 1819067260302524416 |
|---|---|
| author | Ali Javadi Mehdi Rostamirad Mohammad Reza Zand Monfared Fariba Dastjani Farahani Azam Heidarpour ohammad Khodadad Motlagh |
| author_facet | Ali Javadi Mehdi Rostamirad Mohammad Reza Zand Monfared Fariba Dastjani Farahani Azam Heidarpour ohammad Khodadad Motlagh |
| author_sort | Ali Javadi |
| collection | DOAJ |
| description | Background and Objectives: Pseudomonas aeruginosa is a gram-negative bacterium and an opportunistic pathogen with several virulence factors. This bacterium is one of the common pathogens in nosocomial infections, particularly in patients with cystic fibrosis . Also, one of the important mechanisms of the survival of this pathogen in the pulmonary disease is biofilm formation. This study was conducted with the purpose of determining the sub-MIC concentrations of two antibiotics, ampicillin and gentamicin, along with the form conjugated with gold nanoparticles on the formation of biofilms in mucoid strain of Pseudomonas aeruginosa. Methods: In this study, gold nanoparticles were synthesized using trisodium citrate in a chemical reduction method, then the shape and size of gold nanoparticles were determined by transmission electron microscopy (TEM).Conjugation of gold nanoparticles with ampicillin and gentamicin antibiotics was performed by mixing them together. To test the effect of compound son biofilm formation, micro plate method was performed. Results: In this study, approximate size of nanoparticles was determined to be 10nm. In electronic image (in conjugated form), accumulation of nanoparticles was observed in the image. Also, gold nanoparticles conjugated with the drugs, had better effect on biofilm formation compared to free form of the drugs. Conclusion: According to the results of this study, gold nanoparticles by increasing the concentration of drug around themselves result in better inhibitory effect of drug on the biofilm formation compared to the free form of drug. |
| first_indexed | 2024-12-21T16:15:25Z |
| format | Article |
| id | doaj.art-67aa444be47e4f6f94e9542803e649dd |
| institution | Directory Open Access Journal |
| issn | 1735-7799 2008-1375 |
| language | fas |
| last_indexed | 2024-12-21T16:15:25Z |
| publishDate | 2015-07-01 |
| publisher | Qom University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
| spelling | doaj.art-67aa444be47e4f6f94e9542803e649dd2022-12-21T18:57:42ZfasQom University of Medical SciencesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum1735-77992008-13752015-07-01953541The Effect of Ampicillin and Gentamicin Conjugated with Gold Nanoparticles on the Formation of Biofilms in Pseudomonas aeruginosaAli Javadi0Mehdi Rostamirad1Mohammad Reza Zand Monfared2Fariba Dastjani Farahani3Azam Heidarpour4ohammad Khodadad Motlagh5 Tehran University of Medical Sciences Tehran University of Medical Sciences Islamic Azad University Islamic Azad University Qom University of Medical Sciences Shiraz University Background and Objectives: Pseudomonas aeruginosa is a gram-negative bacterium and an opportunistic pathogen with several virulence factors. This bacterium is one of the common pathogens in nosocomial infections, particularly in patients with cystic fibrosis . Also, one of the important mechanisms of the survival of this pathogen in the pulmonary disease is biofilm formation. This study was conducted with the purpose of determining the sub-MIC concentrations of two antibiotics, ampicillin and gentamicin, along with the form conjugated with gold nanoparticles on the formation of biofilms in mucoid strain of Pseudomonas aeruginosa. Methods: In this study, gold nanoparticles were synthesized using trisodium citrate in a chemical reduction method, then the shape and size of gold nanoparticles were determined by transmission electron microscopy (TEM).Conjugation of gold nanoparticles with ampicillin and gentamicin antibiotics was performed by mixing them together. To test the effect of compound son biofilm formation, micro plate method was performed. Results: In this study, approximate size of nanoparticles was determined to be 10nm. In electronic image (in conjugated form), accumulation of nanoparticles was observed in the image. Also, gold nanoparticles conjugated with the drugs, had better effect on biofilm formation compared to free form of the drugs. Conclusion: According to the results of this study, gold nanoparticles by increasing the concentration of drug around themselves result in better inhibitory effect of drug on the biofilm formation compared to the free form of drug.http://journal.muq.ac.ir/article-1-218-en.htmlpseudomonas aeruginosananoparticlesampicillingentamicinsantibiofilm activity |
| spellingShingle | Ali Javadi Mehdi Rostamirad Mohammad Reza Zand Monfared Fariba Dastjani Farahani Azam Heidarpour ohammad Khodadad Motlagh The Effect of Ampicillin and Gentamicin Conjugated with Gold Nanoparticles on the Formation of Biofilms in Pseudomonas aeruginosa Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum pseudomonas aeruginosa nanoparticles ampicillin gentamicins antibiofilm activity |
| title | The Effect of Ampicillin and Gentamicin Conjugated with Gold Nanoparticles on the Formation of Biofilms in Pseudomonas aeruginosa |
| title_full | The Effect of Ampicillin and Gentamicin Conjugated with Gold Nanoparticles on the Formation of Biofilms in Pseudomonas aeruginosa |
| title_fullStr | The Effect of Ampicillin and Gentamicin Conjugated with Gold Nanoparticles on the Formation of Biofilms in Pseudomonas aeruginosa |
| title_full_unstemmed | The Effect of Ampicillin and Gentamicin Conjugated with Gold Nanoparticles on the Formation of Biofilms in Pseudomonas aeruginosa |
| title_short | The Effect of Ampicillin and Gentamicin Conjugated with Gold Nanoparticles on the Formation of Biofilms in Pseudomonas aeruginosa |
| title_sort | effect of ampicillin and gentamicin conjugated with gold nanoparticles on the formation of biofilms in pseudomonas aeruginosa |
| topic | pseudomonas aeruginosa nanoparticles ampicillin gentamicins antibiofilm activity |
| url | http://journal.muq.ac.ir/article-1-218-en.html |
| work_keys_str_mv | AT alijavadi theeffectofampicillinandgentamicinconjugatedwithgoldnanoparticlesontheformationofbiofilmsinpseudomonasaeruginosa AT mehdirostamirad theeffectofampicillinandgentamicinconjugatedwithgoldnanoparticlesontheformationofbiofilmsinpseudomonasaeruginosa AT mohammadrezazandmonfared theeffectofampicillinandgentamicinconjugatedwithgoldnanoparticlesontheformationofbiofilmsinpseudomonasaeruginosa AT faribadastjanifarahani theeffectofampicillinandgentamicinconjugatedwithgoldnanoparticlesontheformationofbiofilmsinpseudomonasaeruginosa AT azamheidarpour theeffectofampicillinandgentamicinconjugatedwithgoldnanoparticlesontheformationofbiofilmsinpseudomonasaeruginosa AT ohammadkhodadadmotlagh theeffectofampicillinandgentamicinconjugatedwithgoldnanoparticlesontheformationofbiofilmsinpseudomonasaeruginosa AT alijavadi effectofampicillinandgentamicinconjugatedwithgoldnanoparticlesontheformationofbiofilmsinpseudomonasaeruginosa AT mehdirostamirad effectofampicillinandgentamicinconjugatedwithgoldnanoparticlesontheformationofbiofilmsinpseudomonasaeruginosa AT mohammadrezazandmonfared effectofampicillinandgentamicinconjugatedwithgoldnanoparticlesontheformationofbiofilmsinpseudomonasaeruginosa AT faribadastjanifarahani effectofampicillinandgentamicinconjugatedwithgoldnanoparticlesontheformationofbiofilmsinpseudomonasaeruginosa AT azamheidarpour effectofampicillinandgentamicinconjugatedwithgoldnanoparticlesontheformationofbiofilmsinpseudomonasaeruginosa AT ohammadkhodadadmotlagh effectofampicillinandgentamicinconjugatedwithgoldnanoparticlesontheformationofbiofilmsinpseudomonasaeruginosa |