THE KINETICS BOTH OF GROWTH AND METABOLITE PRODUCTION OF X.CAMPESTRIS USING OF 4% LIQUID SUGAR SUBSTRATE FROM CASSAVA HYDROLISATE
X. campestris is aerobic bacteria producing extracellular biopolymers (EPS, extracellular polysaccharide) known as xanthan gum. To determine the technology and the process conditions appropriate to the formation of this product, therefore the understanding of both the kinetics of growth and metaboli...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Negeri Semarang
2017-06-01
|
| Series: | Jurnal Bahan Alam Terbarukan |
| Subjects: | |
| Online Access: | https://journal.unnes.ac.id/nju/index.php/jbat/article/view/7950/6331 |
| _version_ | 1818855010191015936 |
|---|---|
| author | Nancy Siti Djenar Edi Wahyu Sri Mulyono |
| author_facet | Nancy Siti Djenar Edi Wahyu Sri Mulyono |
| author_sort | Nancy Siti Djenar |
| collection | DOAJ |
| description | X. campestris is aerobic bacteria producing extracellular biopolymers (EPS, extracellular polysaccharide) known as xanthan gum. To determine the technology and the process conditions appropriate to the formation of this product, therefore the understanding of both the kinetics of growth and metabolite production of bacteria is needed. In this research, to assess the growth of X. campestris using the method of calculation of dry cell weight. For determining the kinetics of production of metabolite used substrates of 4% liquid sugar from cassava starch hydrolysate. From this research was showed that X. campestris maximum growth in NB medium obtained in about 58thhour, at the growth rate of about 0.04 g / hour, stationary phase obtained at the 60th hour with a maximum dry cell weight of 2.7688 g/L and specific growth rate (μ) of X. campestris amounted to 0.043 hour-1. Based on the kinetic curves both on growth and its metabolite production, X.campestris has non-growth associated product pattern. In this case the production of xanthan gum occured after cell growth stopped then its product is a secondary metabolite with highest amount of 3.73 g / L at 102nd hour, ie the 4th day of fermentation. Overall of this research indicated that Nutrient Broth (NB) may be used for the growth of X. campestris. But based on the value of μ above, the rate of cell reproduction was still low. Liquid sugar can be used as a substrate to produce xanthan gum. However to increase its productivity, there should be an addition of other carbon or energy and nitrogen sources. |
| first_indexed | 2024-12-19T08:01:48Z |
| format | Article |
| id | doaj.art-67ab1dca80854347ab3141c701f2742b |
| institution | Directory Open Access Journal |
| issn | 2303-0623 |
| language | English |
| last_indexed | 2024-12-19T08:01:48Z |
| publishDate | 2017-06-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Jurnal Bahan Alam Terbarukan |
| spelling | doaj.art-67ab1dca80854347ab3141c701f2742b2022-12-21T20:29:50ZengUniversitas Negeri SemarangJurnal Bahan Alam Terbarukan2303-06232017-06-01614549THE KINETICS BOTH OF GROWTH AND METABOLITE PRODUCTION OF X.CAMPESTRIS USING OF 4% LIQUID SUGAR SUBSTRATE FROM CASSAVA HYDROLISATENancy Siti Djenar0Edi Wahyu Sri Mulyono1Jurusan Teknik Kimia Politeknik Negeri Bandung Jln. Gegerkalong Hilir, Ds.Ciwaruga, Bandung 40012, Kotak Pos 1234 Jurusan Teknik Kimia Politeknik Negeri Bandung Jln. Gegerkalong Hilir, Ds.Ciwaruga, Bandung 40012, Kotak Pos 1234 X. campestris is aerobic bacteria producing extracellular biopolymers (EPS, extracellular polysaccharide) known as xanthan gum. To determine the technology and the process conditions appropriate to the formation of this product, therefore the understanding of both the kinetics of growth and metabolite production of bacteria is needed. In this research, to assess the growth of X. campestris using the method of calculation of dry cell weight. For determining the kinetics of production of metabolite used substrates of 4% liquid sugar from cassava starch hydrolysate. From this research was showed that X. campestris maximum growth in NB medium obtained in about 58thhour, at the growth rate of about 0.04 g / hour, stationary phase obtained at the 60th hour with a maximum dry cell weight of 2.7688 g/L and specific growth rate (μ) of X. campestris amounted to 0.043 hour-1. Based on the kinetic curves both on growth and its metabolite production, X.campestris has non-growth associated product pattern. In this case the production of xanthan gum occured after cell growth stopped then its product is a secondary metabolite with highest amount of 3.73 g / L at 102nd hour, ie the 4th day of fermentation. Overall of this research indicated that Nutrient Broth (NB) may be used for the growth of X. campestris. But based on the value of μ above, the rate of cell reproduction was still low. Liquid sugar can be used as a substrate to produce xanthan gum. However to increase its productivity, there should be an addition of other carbon or energy and nitrogen sources.https://journal.unnes.ac.id/nju/index.php/jbat/article/view/7950/6331Kineticliquid sugarxanthan gumX. campestris |
| spellingShingle | Nancy Siti Djenar Edi Wahyu Sri Mulyono THE KINETICS BOTH OF GROWTH AND METABOLITE PRODUCTION OF X.CAMPESTRIS USING OF 4% LIQUID SUGAR SUBSTRATE FROM CASSAVA HYDROLISATE Jurnal Bahan Alam Terbarukan Kinetic liquid sugar xanthan gum X. campestris |
| title | THE KINETICS BOTH OF GROWTH AND METABOLITE PRODUCTION OF X.CAMPESTRIS USING OF 4% LIQUID SUGAR SUBSTRATE FROM CASSAVA HYDROLISATE |
| title_full | THE KINETICS BOTH OF GROWTH AND METABOLITE PRODUCTION OF X.CAMPESTRIS USING OF 4% LIQUID SUGAR SUBSTRATE FROM CASSAVA HYDROLISATE |
| title_fullStr | THE KINETICS BOTH OF GROWTH AND METABOLITE PRODUCTION OF X.CAMPESTRIS USING OF 4% LIQUID SUGAR SUBSTRATE FROM CASSAVA HYDROLISATE |
| title_full_unstemmed | THE KINETICS BOTH OF GROWTH AND METABOLITE PRODUCTION OF X.CAMPESTRIS USING OF 4% LIQUID SUGAR SUBSTRATE FROM CASSAVA HYDROLISATE |
| title_short | THE KINETICS BOTH OF GROWTH AND METABOLITE PRODUCTION OF X.CAMPESTRIS USING OF 4% LIQUID SUGAR SUBSTRATE FROM CASSAVA HYDROLISATE |
| title_sort | kinetics both of growth and metabolite production of x campestris using of 4 liquid sugar substrate from cassava hydrolisate |
| topic | Kinetic liquid sugar xanthan gum X. campestris |
| url | https://journal.unnes.ac.id/nju/index.php/jbat/article/view/7950/6331 |
| work_keys_str_mv | AT nancysitidjenar thekineticsbothofgrowthandmetaboliteproductionofxcampestrisusingof4liquidsugarsubstratefromcassavahydrolisate AT ediwahyusrimulyono thekineticsbothofgrowthandmetaboliteproductionofxcampestrisusingof4liquidsugarsubstratefromcassavahydrolisate AT nancysitidjenar kineticsbothofgrowthandmetaboliteproductionofxcampestrisusingof4liquidsugarsubstratefromcassavahydrolisate AT ediwahyusrimulyono kineticsbothofgrowthandmetaboliteproductionofxcampestrisusingof4liquidsugarsubstratefromcassavahydrolisate |