M2 macrophagy-derived exosomal miRNA-26a-5p induces osteogenic differentiation of bone mesenchymal stem cells
Abstract Background Bone marrow mesenchymal stem cells have always been a heated research topic in bone tissue regeneration and repair because of their self-renewal and multi-differentiation potential. A large number of studies have been focused on finding the inducing factors that will promote the...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-03-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13018-022-03029-0 |
| _version_ | 1798005399650041856 |
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| author | Zhang Bin-bin Zha Xi Da-wa Li Chao Zhang Lan-tao Wu Tao Lu Chuan Liu Chao-zheng Li De-chun Feng Chang Wei Shu-qing Dong Zu-nan Pei Xian-wei Zhi-xia Zhang Li Ke-wen |
| author_facet | Zhang Bin-bin Zha Xi Da-wa Li Chao Zhang Lan-tao Wu Tao Lu Chuan Liu Chao-zheng Li De-chun Feng Chang Wei Shu-qing Dong Zu-nan Pei Xian-wei Zhi-xia Zhang Li Ke-wen |
| author_sort | Zhang Bin-bin |
| collection | DOAJ |
| description | Abstract Background Bone marrow mesenchymal stem cells have always been a heated research topic in bone tissue regeneration and repair because of their self-renewal and multi-differentiation potential. A large number of studies have been focused on finding the inducing factors that will promote the osteogenic differentiation of bone marrow mesenchymal stem cells. Previous studies have shown that macrophage exosomes or miRNA-26a-5p can make it work, but the function of this kind of substance on cell osteogenic differentiation has not been public. Methods M2 macrophages are obtained from IL-4 polarized bone marrow-derived macrophages. Exosomes were isolated from the supernatant of M2 macrophages and identified via transmission electron microscopy (TEM), western blotting, and DLS. Chondrogenic differentiation potential was detected by Alcian blue staining. Oil red O staining was used to detect the potential for lipogenic differentiation. And MTT would detect the proliferative capacity of cells. Western blot was performed to detect differential expression of osteogenic differentiation-related proteins. Results The results showed that M2 macrophage exosomes will promote bone differentiation and at the same time inhibit lipid differentiation. In addition, M2 macrophage-derived exosomes have the function of promoting the expression of SOX and Aggrecan suppressing the level of MMP13. The exosome inhibitor GW4689 suppresses miRNA-26a-5p in M2 macrophage exosomes, and the treated exosomes do not play an important role in promoting bone differentiation. Moreover, miRNA-26a-5p can enable to promote bone differentiation and inhibit lipid differentiation. miRNA-26a-5p can promote the expression of ALP (alkaline phosphatase), RUNX-2 (Runt-related transcription factor 2), OPN(osteopontin), and Col-2(collagen type II). Therefore, it is speculated that exosomal miRNA-26a-5p is indispensable in osteogenic differentiation. Conclusions The present study indicated that M2 macrophage exosomes carrying miRNA-26a-5p can induce osteogenic differentiation of bone marrow-derived stem cells to inhibit lipogenic differentiation, and miRNA-26a-5p will also promote the expression of osteogenic differentiation-related proteins ALP, RUNX-2, OPN, and Col-2. |
| first_indexed | 2024-04-11T12:39:45Z |
| format | Article |
| id | doaj.art-67ab5a115c5646a7be382a1fb54701bd |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T12:39:45Z |
| publishDate | 2022-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-67ab5a115c5646a7be382a1fb54701bd2022-12-22T04:23:32ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2022-03-0117111010.1186/s13018-022-03029-0M2 macrophagy-derived exosomal miRNA-26a-5p induces osteogenic differentiation of bone mesenchymal stem cellsZhang Bin-bin0Zha Xi Da-wa1Li Chao2Zhang Lan-tao3Wu Tao4Lu Chuan5Liu Chao-zheng6Li De-chun7Feng Chang8Wei Shu-qing9Dong Zu-nan10Pei Xian-wei11Zhi-xia Zhang12Li Ke-wen13Department of Joint Surgery, Qinghai University Affiliated HospitalDepartment of Joint Surgery, Qinghai University Affiliated HospitalDepartment of Joint Surgery, Qinghai University Affiliated HospitalDepartment of Joint Surgery, Qinghai University Affiliated HospitalDepartment of Joint Surgery, Qinghai University Affiliated HospitalDepartment of Joint Surgery, Qinghai University Affiliated HospitalDepartment of Joint Surgery, Qinghai University Affiliated HospitalDepartment of Joint Surgery, Qinghai University Affiliated HospitalDepartment of Joint Surgery, Qinghai University Affiliated HospitalDepartment of Joint Surgery, Qinghai University Affiliated HospitalDepartment of Joint Surgery, Qinghai University Affiliated HospitalDepartment of Joint Surgery, Qinghai University Affiliated HospitalState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Out-Patient, School of Stomatology, Fourth Military Medical UniversityDepartment of Joint Surgery, Qinghai University Affiliated HospitalAbstract Background Bone marrow mesenchymal stem cells have always been a heated research topic in bone tissue regeneration and repair because of their self-renewal and multi-differentiation potential. A large number of studies have been focused on finding the inducing factors that will promote the osteogenic differentiation of bone marrow mesenchymal stem cells. Previous studies have shown that macrophage exosomes or miRNA-26a-5p can make it work, but the function of this kind of substance on cell osteogenic differentiation has not been public. Methods M2 macrophages are obtained from IL-4 polarized bone marrow-derived macrophages. Exosomes were isolated from the supernatant of M2 macrophages and identified via transmission electron microscopy (TEM), western blotting, and DLS. Chondrogenic differentiation potential was detected by Alcian blue staining. Oil red O staining was used to detect the potential for lipogenic differentiation. And MTT would detect the proliferative capacity of cells. Western blot was performed to detect differential expression of osteogenic differentiation-related proteins. Results The results showed that M2 macrophage exosomes will promote bone differentiation and at the same time inhibit lipid differentiation. In addition, M2 macrophage-derived exosomes have the function of promoting the expression of SOX and Aggrecan suppressing the level of MMP13. The exosome inhibitor GW4689 suppresses miRNA-26a-5p in M2 macrophage exosomes, and the treated exosomes do not play an important role in promoting bone differentiation. Moreover, miRNA-26a-5p can enable to promote bone differentiation and inhibit lipid differentiation. miRNA-26a-5p can promote the expression of ALP (alkaline phosphatase), RUNX-2 (Runt-related transcription factor 2), OPN(osteopontin), and Col-2(collagen type II). Therefore, it is speculated that exosomal miRNA-26a-5p is indispensable in osteogenic differentiation. Conclusions The present study indicated that M2 macrophage exosomes carrying miRNA-26a-5p can induce osteogenic differentiation of bone marrow-derived stem cells to inhibit lipogenic differentiation, and miRNA-26a-5p will also promote the expression of osteogenic differentiation-related proteins ALP, RUNX-2, OPN, and Col-2.https://doi.org/10.1186/s13018-022-03029-0ExosomesmiRNA-26a-5pMacrophagesOsteogenic differentiation |
| spellingShingle | Zhang Bin-bin Zha Xi Da-wa Li Chao Zhang Lan-tao Wu Tao Lu Chuan Liu Chao-zheng Li De-chun Feng Chang Wei Shu-qing Dong Zu-nan Pei Xian-wei Zhi-xia Zhang Li Ke-wen M2 macrophagy-derived exosomal miRNA-26a-5p induces osteogenic differentiation of bone mesenchymal stem cells Journal of Orthopaedic Surgery and Research Exosomes miRNA-26a-5p Macrophages Osteogenic differentiation |
| title | M2 macrophagy-derived exosomal miRNA-26a-5p induces osteogenic differentiation of bone mesenchymal stem cells |
| title_full | M2 macrophagy-derived exosomal miRNA-26a-5p induces osteogenic differentiation of bone mesenchymal stem cells |
| title_fullStr | M2 macrophagy-derived exosomal miRNA-26a-5p induces osteogenic differentiation of bone mesenchymal stem cells |
| title_full_unstemmed | M2 macrophagy-derived exosomal miRNA-26a-5p induces osteogenic differentiation of bone mesenchymal stem cells |
| title_short | M2 macrophagy-derived exosomal miRNA-26a-5p induces osteogenic differentiation of bone mesenchymal stem cells |
| title_sort | m2 macrophagy derived exosomal mirna 26a 5p induces osteogenic differentiation of bone mesenchymal stem cells |
| topic | Exosomes miRNA-26a-5p Macrophages Osteogenic differentiation |
| url | https://doi.org/10.1186/s13018-022-03029-0 |
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