Regulation of seed oil accumulation by lncRNAs in Brassica napus
Abstract Background Studies have indicated that long non-coding RNAs (lncRNAs) play important regulatory roles in many biological processes. However, the regulation of seed oil biosynthesis by lncRNAs remains largely unknown. Results We comprehensively identified and characterized the lncRNAs from s...
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-022-02256-1 |
| _version_ | 1811166003498844160 |
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| author | Yuqing Li Zengdong Tan Chenghao Zeng Mengying Xiao Shengli Lin Wei Yao Qing Li Liang Guo Shaoping Lu |
| author_facet | Yuqing Li Zengdong Tan Chenghao Zeng Mengying Xiao Shengli Lin Wei Yao Qing Li Liang Guo Shaoping Lu |
| author_sort | Yuqing Li |
| collection | DOAJ |
| description | Abstract Background Studies have indicated that long non-coding RNAs (lncRNAs) play important regulatory roles in many biological processes. However, the regulation of seed oil biosynthesis by lncRNAs remains largely unknown. Results We comprehensively identified and characterized the lncRNAs from seeds in three developing stages in two accessions of Brassica napus (B. napus), ZS11 (high oil content) and WH5557 (low oil content). Finally, 8094 expressed lncRNAs were identified. LncRNAs MSTRG.22563 and MSTRG.86004 were predicted to be related to seed oil accumulation. Experimental results show that the seed oil content is decreased by 3.1–3.9% in MSTRG.22563 overexpression plants, while increased about 2% in MSTRG.86004, compared to WT. Further study showed that most genes related to lipid metabolism had much lower expression, and the content of some metabolites in the processes of respiration and TCA (tricarboxylic acid) cycle was reduced in MSTRG.22563 transgenic seeds. The expression of genes involved in fatty acid synthesis and seed embryonic development (e.g., LEC1) was increased, but genes related to TAG assembly was decreased in MSTRG.86004 transgenic seeds. Conclusion Our results suggest that MSTRG.22563 might impact seed oil content by affecting the respiration and TCA cycle, while MSTRG.86004 plays a role in prolonging the seed developmental time to increase seed oil accumulation. |
| first_indexed | 2024-04-10T15:46:24Z |
| format | Article |
| id | doaj.art-67aba2c8446b468ea9826f95889975c0 |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-04-10T15:46:24Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-67aba2c8446b468ea9826f95889975c02023-02-12T12:06:42ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542023-02-0116111910.1186/s13068-022-02256-1Regulation of seed oil accumulation by lncRNAs in Brassica napusYuqing Li0Zengdong Tan1Chenghao Zeng2Mengying Xiao3Shengli Lin4Wei Yao5Qing Li6Liang Guo7Shaoping Lu8National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityAbstract Background Studies have indicated that long non-coding RNAs (lncRNAs) play important regulatory roles in many biological processes. However, the regulation of seed oil biosynthesis by lncRNAs remains largely unknown. Results We comprehensively identified and characterized the lncRNAs from seeds in three developing stages in two accessions of Brassica napus (B. napus), ZS11 (high oil content) and WH5557 (low oil content). Finally, 8094 expressed lncRNAs were identified. LncRNAs MSTRG.22563 and MSTRG.86004 were predicted to be related to seed oil accumulation. Experimental results show that the seed oil content is decreased by 3.1–3.9% in MSTRG.22563 overexpression plants, while increased about 2% in MSTRG.86004, compared to WT. Further study showed that most genes related to lipid metabolism had much lower expression, and the content of some metabolites in the processes of respiration and TCA (tricarboxylic acid) cycle was reduced in MSTRG.22563 transgenic seeds. The expression of genes involved in fatty acid synthesis and seed embryonic development (e.g., LEC1) was increased, but genes related to TAG assembly was decreased in MSTRG.86004 transgenic seeds. Conclusion Our results suggest that MSTRG.22563 might impact seed oil content by affecting the respiration and TCA cycle, while MSTRG.86004 plays a role in prolonging the seed developmental time to increase seed oil accumulation.https://doi.org/10.1186/s13068-022-02256-1LncRNAOil contentLipidMetaboliteTransgenic plantsBrassica napus |
| spellingShingle | Yuqing Li Zengdong Tan Chenghao Zeng Mengying Xiao Shengli Lin Wei Yao Qing Li Liang Guo Shaoping Lu Regulation of seed oil accumulation by lncRNAs in Brassica napus Biotechnology for Biofuels and Bioproducts LncRNA Oil content Lipid Metabolite Transgenic plants Brassica napus |
| title | Regulation of seed oil accumulation by lncRNAs in Brassica napus |
| title_full | Regulation of seed oil accumulation by lncRNAs in Brassica napus |
| title_fullStr | Regulation of seed oil accumulation by lncRNAs in Brassica napus |
| title_full_unstemmed | Regulation of seed oil accumulation by lncRNAs in Brassica napus |
| title_short | Regulation of seed oil accumulation by lncRNAs in Brassica napus |
| title_sort | regulation of seed oil accumulation by lncrnas in brassica napus |
| topic | LncRNA Oil content Lipid Metabolite Transgenic plants Brassica napus |
| url | https://doi.org/10.1186/s13068-022-02256-1 |
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