Association of Interleukin-27 gene rs153109 polymorphism and chronic hepatitis B infection
Background: According to World Health Organization (WHO) report about 400 million people are chronically infected with hepatitis B virus (HBV). Host immune responses which are mainly controlled by cytokines, can be either effective in disease progression or control the infection. Interleukin-27 (IL-...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Kashan University of Medical Sciences and Health Services
2017-04-01
|
| Series: | Fiyz̤ |
| Subjects: | |
| Online Access: | http://feyz.kaums.ac.ir/article-1-3280-en.html |
| _version_ | 1818050945672544256 |
|---|---|
| author | Samira Mokhtari Seyyed Masud Hosseini Seyyed Reza Mohebbi Pedram Azimzadeh Mohammad Reza Zali |
| author_facet | Samira Mokhtari Seyyed Masud Hosseini Seyyed Reza Mohebbi Pedram Azimzadeh Mohammad Reza Zali |
| author_sort | Samira Mokhtari |
| collection | DOAJ |
| description | Background: According to World Health Organization (WHO) report about 400 million people are chronically infected with hepatitis B virus (HBV). Host immune responses which are mainly controlled by cytokines, can be either effective in disease progression or control the infection. Interleukin-27 (IL-27) is a pro-inflammatory cytokine which promotes Th1 responses. Genetic variations (e.g. single nucleotide polymorphisms [SNPs]) can affect the product or activity of IL-27 gene. The aim of present study was to determine the association between IL-27 rs153109 and chronic HBV infection among the Iranian population.
Materials and Methods: In this study chronic HBV patients (n=120, Anti-HBc Ab positive and HBsAg positive for more than 6 months) and controls (n=120) from healthy individuals referred to Tehran Taleghani hospital (2013-2014) were studied. Genotypes of IL-27 gene polymorphism were detected by PCR-RFLP. DNA sequencing was applied on 10% of samples to validate the genotyping results. The studied variables were polymorphism genotypes/alleles, clinical status, age and gender.
Results: Results showed no statistically significant difference for patients and control groups neither in genotype frequencies of AA among the chronic group (30%) compared to healthy controls (32.5%) (P=0.368); nor in allele frequency A) 60.4%) for patients against A 59.2% in control groups (P=0.780).
Conclusion: Despite the importance of IL-27 in the immune response, the findings of this study suggests that genetic variants of IL-27 SNP 153109A/G were not associated with susceptibility to the chronic infection of HBV. |
| first_indexed | 2024-12-10T11:01:32Z |
| format | Article |
| id | doaj.art-67ac2202f2d947dcb76c8f1e79763f07 |
| institution | Directory Open Access Journal |
| issn | 1029-7855 2008-9821 |
| language | fas |
| last_indexed | 2024-12-10T11:01:32Z |
| publishDate | 2017-04-01 |
| publisher | Kashan University of Medical Sciences and Health Services |
| record_format | Article |
| series | Fiyz̤ |
| spelling | doaj.art-67ac2202f2d947dcb76c8f1e79763f072022-12-22T01:51:40ZfasKashan University of Medical Sciences and Health ServicesFiyz̤1029-78552008-98212017-04-012113541Association of Interleukin-27 gene rs153109 polymorphism and chronic hepatitis B infectionSamira Mokhtari 0 Seyyed Masud Hosseini 1Seyyed Reza Mohebbi 2Pedram Azimzadeh 3 Mohammad Reza Zali 4Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, I. R. Iran.Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, I. R. Iran.Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, I. R. Iran.Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, I. R. Iran.Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, I. R. Iran.Background: According to World Health Organization (WHO) report about 400 million people are chronically infected with hepatitis B virus (HBV). Host immune responses which are mainly controlled by cytokines, can be either effective in disease progression or control the infection. Interleukin-27 (IL-27) is a pro-inflammatory cytokine which promotes Th1 responses. Genetic variations (e.g. single nucleotide polymorphisms [SNPs]) can affect the product or activity of IL-27 gene. The aim of present study was to determine the association between IL-27 rs153109 and chronic HBV infection among the Iranian population. Materials and Methods: In this study chronic HBV patients (n=120, Anti-HBc Ab positive and HBsAg positive for more than 6 months) and controls (n=120) from healthy individuals referred to Tehran Taleghani hospital (2013-2014) were studied. Genotypes of IL-27 gene polymorphism were detected by PCR-RFLP. DNA sequencing was applied on 10% of samples to validate the genotyping results. The studied variables were polymorphism genotypes/alleles, clinical status, age and gender. Results: Results showed no statistically significant difference for patients and control groups neither in genotype frequencies of AA among the chronic group (30%) compared to healthy controls (32.5%) (P=0.368); nor in allele frequency A) 60.4%) for patients against A 59.2% in control groups (P=0.780). Conclusion: Despite the importance of IL-27 in the immune response, the findings of this study suggests that genetic variants of IL-27 SNP 153109A/G were not associated with susceptibility to the chronic infection of HBV.http://feyz.kaums.ac.ir/article-1-3280-en.htmlChronic HBV infectionIL-27SNPrs153109 |
| spellingShingle | Samira Mokhtari Seyyed Masud Hosseini Seyyed Reza Mohebbi Pedram Azimzadeh Mohammad Reza Zali Association of Interleukin-27 gene rs153109 polymorphism and chronic hepatitis B infection Fiyz̤ Chronic HBV infection IL-27 SNP rs153109 |
| title | Association of Interleukin-27 gene rs153109 polymorphism and chronic hepatitis B infection |
| title_full | Association of Interleukin-27 gene rs153109 polymorphism and chronic hepatitis B infection |
| title_fullStr | Association of Interleukin-27 gene rs153109 polymorphism and chronic hepatitis B infection |
| title_full_unstemmed | Association of Interleukin-27 gene rs153109 polymorphism and chronic hepatitis B infection |
| title_short | Association of Interleukin-27 gene rs153109 polymorphism and chronic hepatitis B infection |
| title_sort | association of interleukin 27 gene rs153109 polymorphism and chronic hepatitis b infection |
| topic | Chronic HBV infection IL-27 SNP rs153109 |
| url | http://feyz.kaums.ac.ir/article-1-3280-en.html |
| work_keys_str_mv | AT samiramokhtari associationofinterleukin27geners153109polymorphismandchronichepatitisbinfection AT seyyedmasudhosseini associationofinterleukin27geners153109polymorphismandchronichepatitisbinfection AT seyyedrezamohebbi associationofinterleukin27geners153109polymorphismandchronichepatitisbinfection AT pedramazimzadeh associationofinterleukin27geners153109polymorphismandchronichepatitisbinfection AT mohammadrezazali associationofinterleukin27geners153109polymorphismandchronichepatitisbinfection |