Protective Effect and Mechanism of Fucoidan on Alcohol-Induced Liver Injury in Mice
Objective: To investigate the protective effect and potential molecular mechanism of fucoidan on alcoholic liver injury. Methods: A mouse model of alcoholic liver disease (ALD) was established using 50% (V/V) alcohol, and then administered with 300 mg/kg mb of fucoidan. Liver histomorphological chan...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2023-02-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-3-016.pdf |
| _version_ | 1811159494955106304 |
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| author | YANG Jia, DANG Kai, XUE Meilan, LIANG Hui, ZHANG Nan, WANG Qing, PEI Zhongqian, QIN Yimin |
| author_facet | YANG Jia, DANG Kai, XUE Meilan, LIANG Hui, ZHANG Nan, WANG Qing, PEI Zhongqian, QIN Yimin |
| author_sort | YANG Jia, DANG Kai, XUE Meilan, LIANG Hui, ZHANG Nan, WANG Qing, PEI Zhongqian, QIN Yimin |
| collection | DOAJ |
| description | Objective: To investigate the protective effect and potential molecular mechanism of fucoidan on alcoholic liver injury. Methods: A mouse model of alcoholic liver disease (ALD) was established using 50% (V/V) alcohol, and then administered with 300 mg/kg mb of fucoidan. Liver histomorphological changes were compared in the control, ALD and fucoidan intervention groups, and the levels of serum aminotransferase, serum lipids, and inflammatory factors, the proportion of T helper (Th) cells including Th1, Th2 and Th17, and the expression levels of autophagy-related proteins and microtubule-associated protein 1 light chain 3 beta (LC3B) were detected to explore the mechanism by which fucoidan can improve alcoholic liver injury. Results: Fucoidan could improve the pathological changes of liver tissue, reduce the levels of serum alanine (aminotransferase (ALT) and aspartate aminotransferase (AST)) and blood lipids (triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and total bile acid (TBA)) (P < 0.05), and decrease the levels of serum inflammatory factors (TNF-α, IL-1β and IL-6) and the proportion of Th1, Th2 and Th17 (P < 0.05). Meanwhile, it could down-regulate the expression of autophagy-related protein p62, mammalian target of rapamycin complex 1 (mTORC1) and ribosomal protein 70S6 kinase (p70S6K) (P < 0.05), promote the nuclear translocation of transcription factor EB (TFEB) (P < 0.05), and up-regulate the expression of LC3B II protein and LC3B fluorescent protein (P < 0.05). Conclusion: Fucoidan can alleviate lipotoxicity and inflammatory injury in the liver of ALD mice, and its mechanism may be related to the activation of autophagy. |
| first_indexed | 2024-04-10T05:42:06Z |
| format | Article |
| id | doaj.art-67adf84929d94c67be1a06663dde61e5 |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-04-10T05:42:06Z |
| publishDate | 2023-02-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-67adf84929d94c67be1a06663dde61e52023-03-06T07:13:22ZengChina Food Publishing CompanyShipin Kexue1002-66302023-02-0144313714510.7506/spkx1002-6630-20220322-276Protective Effect and Mechanism of Fucoidan on Alcohol-Induced Liver Injury in MiceYANG Jia, DANG Kai, XUE Meilan, LIANG Hui, ZHANG Nan, WANG Qing, PEI Zhongqian, QIN Yimin0(1. School of Basic Medicine, Qingdao University, Qingdao 266071, China;2. School of Public Health, Qingdao University, Qingdao 266071, China; 3. Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao 266021, China; 4. State Key Laboratory of Bioactive Seaweed Substances, Qingdao Brightmoon Seaweed Group Co., Ltd., Qingdao 266555, China)Objective: To investigate the protective effect and potential molecular mechanism of fucoidan on alcoholic liver injury. Methods: A mouse model of alcoholic liver disease (ALD) was established using 50% (V/V) alcohol, and then administered with 300 mg/kg mb of fucoidan. Liver histomorphological changes were compared in the control, ALD and fucoidan intervention groups, and the levels of serum aminotransferase, serum lipids, and inflammatory factors, the proportion of T helper (Th) cells including Th1, Th2 and Th17, and the expression levels of autophagy-related proteins and microtubule-associated protein 1 light chain 3 beta (LC3B) were detected to explore the mechanism by which fucoidan can improve alcoholic liver injury. Results: Fucoidan could improve the pathological changes of liver tissue, reduce the levels of serum alanine (aminotransferase (ALT) and aspartate aminotransferase (AST)) and blood lipids (triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and total bile acid (TBA)) (P < 0.05), and decrease the levels of serum inflammatory factors (TNF-α, IL-1β and IL-6) and the proportion of Th1, Th2 and Th17 (P < 0.05). Meanwhile, it could down-regulate the expression of autophagy-related protein p62, mammalian target of rapamycin complex 1 (mTORC1) and ribosomal protein 70S6 kinase (p70S6K) (P < 0.05), promote the nuclear translocation of transcription factor EB (TFEB) (P < 0.05), and up-regulate the expression of LC3B II protein and LC3B fluorescent protein (P < 0.05). Conclusion: Fucoidan can alleviate lipotoxicity and inflammatory injury in the liver of ALD mice, and its mechanism may be related to the activation of autophagy.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-3-016.pdfautophagy; ethanol-induced liver injury; fucoidan; blood lipids; inflammation |
| spellingShingle | YANG Jia, DANG Kai, XUE Meilan, LIANG Hui, ZHANG Nan, WANG Qing, PEI Zhongqian, QIN Yimin Protective Effect and Mechanism of Fucoidan on Alcohol-Induced Liver Injury in Mice Shipin Kexue autophagy; ethanol-induced liver injury; fucoidan; blood lipids; inflammation |
| title | Protective Effect and Mechanism of Fucoidan on Alcohol-Induced Liver Injury in Mice |
| title_full | Protective Effect and Mechanism of Fucoidan on Alcohol-Induced Liver Injury in Mice |
| title_fullStr | Protective Effect and Mechanism of Fucoidan on Alcohol-Induced Liver Injury in Mice |
| title_full_unstemmed | Protective Effect and Mechanism of Fucoidan on Alcohol-Induced Liver Injury in Mice |
| title_short | Protective Effect and Mechanism of Fucoidan on Alcohol-Induced Liver Injury in Mice |
| title_sort | protective effect and mechanism of fucoidan on alcohol induced liver injury in mice |
| topic | autophagy; ethanol-induced liver injury; fucoidan; blood lipids; inflammation |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-3-016.pdf |
| work_keys_str_mv | AT yangjiadangkaixuemeilanlianghuizhangnanwangqingpeizhongqianqinyimin protectiveeffectandmechanismoffucoidanonalcoholinducedliverinjuryinmice |