Anti-inflammatory activity of di-peptides derived from ovotransferrin by simulated peptide-cut in TNF-α-induced Caco-2 cells
Proteins and bioactive peptides in avian egg whites exert anti-inflammatory activity. Here, we aimed to evaluate the anti-inflammatory effect of ovotransferrin (OVT) and its di-peptides by simulated peptide-cut using an in vitro model of TNF-α-induced inflammation in Caco-2 cells. Results showed tha...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-10-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464617304528 |
| _version_ | 1818415615272026112 |
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| author | Xiong Wang Yan Zhao Yao Yao Mingsheng Xu Huaying Du Mengya Zhang Yonggang Tu |
| author_facet | Xiong Wang Yan Zhao Yao Yao Mingsheng Xu Huaying Du Mengya Zhang Yonggang Tu |
| author_sort | Xiong Wang |
| collection | DOAJ |
| description | Proteins and bioactive peptides in avian egg whites exert anti-inflammatory activity. Here, we aimed to evaluate the anti-inflammatory effect of ovotransferrin (OVT) and its di-peptides by simulated peptide-cut using an in vitro model of TNF-α-induced inflammation in Caco-2 cells. Results showed that co-treatment with OVT can’t inhibit the IL-8 secretion in TNF-α-induced Caco-2 cells. Among 18 di-peptides derived from OVT based on simulated peptide-cut, CR, FL, HC, LL and MK markedly decreased IL-8 secretion. Furthermore, these five di-peptides were able to significantly inhibited gene expression of pro-inflammatory cytokines, including TNF-α, IL-8, IL-6, IL-1β, and IL-12, and promoted gene expression of anti-inflammatory cytokines, such as IL-10, in Caco-2 cells. CR, FL, HC, LL and MK inhibited the phosphorylation of inhibitor of κB (IκB). Meanwhile, CR and HC decreased the phosphorylation of p-JNK and p-p-38. These results indicated that CR and HC inhibited the NF-κB and MAPK signaling pathway in TNF-α-induced Caco-2 cells. Nevertheless, LL, FL and MK exerted their anti-inflammatory activity via MAPK signaling pathway. The study suggested that CR, FL, HC, LL and MK have the potential to attenuate intestinal inflammation. |
| first_indexed | 2024-12-14T11:37:48Z |
| format | Article |
| id | doaj.art-67aee8c6c17e48178448579c24d3963c |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T11:37:48Z |
| publishDate | 2017-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-67aee8c6c17e48178448579c24d3963c2022-12-21T23:02:56ZengElsevierJournal of Functional Foods1756-46462017-10-0137424432Anti-inflammatory activity of di-peptides derived from ovotransferrin by simulated peptide-cut in TNF-α-induced Caco-2 cellsXiong Wang0Yan Zhao1Yao Yao2Mingsheng Xu3Huaying Du4Mengya Zhang5Yonggang Tu6Jiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang 330045, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; Engineering Research Centre of Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, ChinaJiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang 330045, ChinaJiangxi Key Laboratory of Natural Products and Functional Food, Jiangxi Agricultural University, Nanchang 330045, China; Corresponding author.Proteins and bioactive peptides in avian egg whites exert anti-inflammatory activity. Here, we aimed to evaluate the anti-inflammatory effect of ovotransferrin (OVT) and its di-peptides by simulated peptide-cut using an in vitro model of TNF-α-induced inflammation in Caco-2 cells. Results showed that co-treatment with OVT can’t inhibit the IL-8 secretion in TNF-α-induced Caco-2 cells. Among 18 di-peptides derived from OVT based on simulated peptide-cut, CR, FL, HC, LL and MK markedly decreased IL-8 secretion. Furthermore, these five di-peptides were able to significantly inhibited gene expression of pro-inflammatory cytokines, including TNF-α, IL-8, IL-6, IL-1β, and IL-12, and promoted gene expression of anti-inflammatory cytokines, such as IL-10, in Caco-2 cells. CR, FL, HC, LL and MK inhibited the phosphorylation of inhibitor of κB (IκB). Meanwhile, CR and HC decreased the phosphorylation of p-JNK and p-p-38. These results indicated that CR and HC inhibited the NF-κB and MAPK signaling pathway in TNF-α-induced Caco-2 cells. Nevertheless, LL, FL and MK exerted their anti-inflammatory activity via MAPK signaling pathway. The study suggested that CR, FL, HC, LL and MK have the potential to attenuate intestinal inflammation.http://www.sciencedirect.com/science/article/pii/S1756464617304528OvotransferrinDi-peptideCaco-2 cellsSimulated digestionAnti-inflammatory |
| spellingShingle | Xiong Wang Yan Zhao Yao Yao Mingsheng Xu Huaying Du Mengya Zhang Yonggang Tu Anti-inflammatory activity of di-peptides derived from ovotransferrin by simulated peptide-cut in TNF-α-induced Caco-2 cells Journal of Functional Foods Ovotransferrin Di-peptide Caco-2 cells Simulated digestion Anti-inflammatory |
| title | Anti-inflammatory activity of di-peptides derived from ovotransferrin by simulated peptide-cut in TNF-α-induced Caco-2 cells |
| title_full | Anti-inflammatory activity of di-peptides derived from ovotransferrin by simulated peptide-cut in TNF-α-induced Caco-2 cells |
| title_fullStr | Anti-inflammatory activity of di-peptides derived from ovotransferrin by simulated peptide-cut in TNF-α-induced Caco-2 cells |
| title_full_unstemmed | Anti-inflammatory activity of di-peptides derived from ovotransferrin by simulated peptide-cut in TNF-α-induced Caco-2 cells |
| title_short | Anti-inflammatory activity of di-peptides derived from ovotransferrin by simulated peptide-cut in TNF-α-induced Caco-2 cells |
| title_sort | anti inflammatory activity of di peptides derived from ovotransferrin by simulated peptide cut in tnf α induced caco 2 cells |
| topic | Ovotransferrin Di-peptide Caco-2 cells Simulated digestion Anti-inflammatory |
| url | http://www.sciencedirect.com/science/article/pii/S1756464617304528 |
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