Expression Regulation of Polycistronic lee3 Genes of Enterohaemorrhagic Escherichia coli.
Enterohaemorrhagic Escherichia coli O157:H7 (EHEC) carries a pathogenic island LEE that is consisted mainly of five polycistronic operons. In the lee3 operon, mpc is the first gene and has been reported to down regulate the type-3 secretion system of EHEC when its gene product is over-expressed. Fur...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0155578&type=printable |
| _version_ | 1826583998772543488 |
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| author | Wei-Sheng W Sun Jenn-Wei Chen Yi-Chih Wu Hsing-Yuan Tsai Yu-Liang Kuo Wan-Jr Syu |
| author_facet | Wei-Sheng W Sun Jenn-Wei Chen Yi-Chih Wu Hsing-Yuan Tsai Yu-Liang Kuo Wan-Jr Syu |
| author_sort | Wei-Sheng W Sun |
| collection | DOAJ |
| description | Enterohaemorrhagic Escherichia coli O157:H7 (EHEC) carries a pathogenic island LEE that is consisted mainly of five polycistronic operons. In the lee3 operon, mpc is the first gene and has been reported to down regulate the type-3 secretion system of EHEC when its gene product is over-expressed. Furthermore, mpc has been suggested to have a regulation function via translation but the mechanism remains unclear. To clarify this hypothesis, we dissected the polycistron and examined the translated products. We conclude that translation of mpc detrimentally governs the translation of the second gene, escV, which in turn affects the translation of the third gene, escN. Then sequentially, escN affects the expression of the downstream genes. Furthermore, we located a critical cis element within the mpc open-reading frame that plays a negative role in the translation-dependent regulation of lee3. Using qRT-PCR, we found that the amount of mpc RNA transcript present in EHEC was relatively limited when compared to any other genes within lee3. Taken together, when the transcription of LEE is activated, expression of mpc is tightly controlled by a restriction of the RNA transcript of mpc, translation of which is then critical for the efficient production of the operon's downstream gene products. |
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| format | Article |
| id | doaj.art-67b020d3730245e483c98968ea0f0aa4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T15:30:46Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-67b020d3730245e483c98968ea0f0aa42025-02-25T05:36:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01115e015557810.1371/journal.pone.0155578Expression Regulation of Polycistronic lee3 Genes of Enterohaemorrhagic Escherichia coli.Wei-Sheng W SunJenn-Wei ChenYi-Chih WuHsing-Yuan TsaiYu-Liang KuoWan-Jr SyuEnterohaemorrhagic Escherichia coli O157:H7 (EHEC) carries a pathogenic island LEE that is consisted mainly of five polycistronic operons. In the lee3 operon, mpc is the first gene and has been reported to down regulate the type-3 secretion system of EHEC when its gene product is over-expressed. Furthermore, mpc has been suggested to have a regulation function via translation but the mechanism remains unclear. To clarify this hypothesis, we dissected the polycistron and examined the translated products. We conclude that translation of mpc detrimentally governs the translation of the second gene, escV, which in turn affects the translation of the third gene, escN. Then sequentially, escN affects the expression of the downstream genes. Furthermore, we located a critical cis element within the mpc open-reading frame that plays a negative role in the translation-dependent regulation of lee3. Using qRT-PCR, we found that the amount of mpc RNA transcript present in EHEC was relatively limited when compared to any other genes within lee3. Taken together, when the transcription of LEE is activated, expression of mpc is tightly controlled by a restriction of the RNA transcript of mpc, translation of which is then critical for the efficient production of the operon's downstream gene products.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0155578&type=printable |
| spellingShingle | Wei-Sheng W Sun Jenn-Wei Chen Yi-Chih Wu Hsing-Yuan Tsai Yu-Liang Kuo Wan-Jr Syu Expression Regulation of Polycistronic lee3 Genes of Enterohaemorrhagic Escherichia coli. PLoS ONE |
| title | Expression Regulation of Polycistronic lee3 Genes of Enterohaemorrhagic Escherichia coli. |
| title_full | Expression Regulation of Polycistronic lee3 Genes of Enterohaemorrhagic Escherichia coli. |
| title_fullStr | Expression Regulation of Polycistronic lee3 Genes of Enterohaemorrhagic Escherichia coli. |
| title_full_unstemmed | Expression Regulation of Polycistronic lee3 Genes of Enterohaemorrhagic Escherichia coli. |
| title_short | Expression Regulation of Polycistronic lee3 Genes of Enterohaemorrhagic Escherichia coli. |
| title_sort | expression regulation of polycistronic lee3 genes of enterohaemorrhagic escherichia coli |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0155578&type=printable |
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