Eco-HAB as a fully automated and ecologically relevant assessment of social impairments in mouse models of autism
Eco-HAB is an open source, RFID-based system for automated measurement and analysis of social preference and in-cohort sociability in mice. The system closely follows murine ethology. It requires no contact between a human experimenter and tested animals, overcoming the confounding factors that lead...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-10-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/19532 |
| _version_ | 1811181457945657344 |
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| author | Alicja Puścian Szymon Łęski Grzegorz Kasprowicz Maciej Winiarski Joanna Borowska Tomasz Nikolaev Paweł M Boguszewski Hans-Peter Lipp Ewelina Knapska |
| author_facet | Alicja Puścian Szymon Łęski Grzegorz Kasprowicz Maciej Winiarski Joanna Borowska Tomasz Nikolaev Paweł M Boguszewski Hans-Peter Lipp Ewelina Knapska |
| author_sort | Alicja Puścian |
| collection | DOAJ |
| description | Eco-HAB is an open source, RFID-based system for automated measurement and analysis of social preference and in-cohort sociability in mice. The system closely follows murine ethology. It requires no contact between a human experimenter and tested animals, overcoming the confounding factors that lead to irreproducible assessment of murine social behavior between laboratories. In Eco-HAB, group-housed animals live in a spacious, four-compartment apparatus with shadowed areas and narrow tunnels, resembling natural burrows. Eco-HAB allows for assessment of the tendency of mice to voluntarily spend time together in ethologically relevant mouse group sizes. Custom-made software for automated tracking, data extraction, and analysis enables quick evaluation of social impairments. The developed protocols and standardized behavioral measures demonstrate high replicability. Unlike classic three-chambered sociability tests, Eco-HAB provides measurements of spontaneous, ecologically relevant social behaviors in group-housed animals. Results are obtained faster, with less manpower, and without confounding factors. |
| first_indexed | 2024-04-11T09:18:04Z |
| format | Article |
| id | doaj.art-67b4b0bf1bc94cda9a6af60b7c11a5e2 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:18:04Z |
| publishDate | 2016-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-67b4b0bf1bc94cda9a6af60b7c11a5e22022-12-22T04:32:17ZengeLife Sciences Publications LtdeLife2050-084X2016-10-01510.7554/eLife.19532Eco-HAB as a fully automated and ecologically relevant assessment of social impairments in mouse models of autismAlicja Puścian0https://orcid.org/0000-0002-7029-1275Szymon Łęski1https://orcid.org/0000-0003-1764-1907Grzegorz Kasprowicz2Maciej Winiarski3Joanna Borowska4Tomasz Nikolaev5Paweł M Boguszewski6https://orcid.org/0000-0002-7210-6950Hans-Peter Lipp7Ewelina Knapska8https://orcid.org/0000-0001-9319-2176Department of Neurophysiology, Nencki Institute of Experimental Biology of Polish Academy of Sciences, Warsaw, PolandDepartment of Neurophysiology, Nencki Institute of Experimental Biology of Polish Academy of Sciences, Warsaw, PolandCenter for Theoretical Physics, Polish Academy of Sciences, Warsaw, Poland; Institute of Electronic Systems, Warsaw University of Technology, Warsaw, PolandDepartment of Neurophysiology, Nencki Institute of Experimental Biology of Polish Academy of Sciences, Warsaw, PolandDepartment of Neurophysiology, Nencki Institute of Experimental Biology of Polish Academy of Sciences, Warsaw, PolandDepartment of Neurophysiology, Nencki Institute of Experimental Biology of Polish Academy of Sciences, Warsaw, PolandDepartment of Neurophysiology, Nencki Institute of Experimental Biology of Polish Academy of Sciences, Warsaw, PolandInstitute of Anatomy, University of Zurich, Zurich, Switzerland; School of Laboratory Medicine, Kwazulu-Natal University Durban, Durban, Republic of South AfricaDepartment of Neurophysiology, Nencki Institute of Experimental Biology of Polish Academy of Sciences, Warsaw, PolandEco-HAB is an open source, RFID-based system for automated measurement and analysis of social preference and in-cohort sociability in mice. The system closely follows murine ethology. It requires no contact between a human experimenter and tested animals, overcoming the confounding factors that lead to irreproducible assessment of murine social behavior between laboratories. In Eco-HAB, group-housed animals live in a spacious, four-compartment apparatus with shadowed areas and narrow tunnels, resembling natural burrows. Eco-HAB allows for assessment of the tendency of mice to voluntarily spend time together in ethologically relevant mouse group sizes. Custom-made software for automated tracking, data extraction, and analysis enables quick evaluation of social impairments. The developed protocols and standardized behavioral measures demonstrate high replicability. Unlike classic three-chambered sociability tests, Eco-HAB provides measurements of spontaneous, ecologically relevant social behaviors in group-housed animals. Results are obtained faster, with less manpower, and without confounding factors.https://elifesciences.org/articles/19532social impairmentssocial behaviormouse modelsautomated testingautismecological relevance |
| spellingShingle | Alicja Puścian Szymon Łęski Grzegorz Kasprowicz Maciej Winiarski Joanna Borowska Tomasz Nikolaev Paweł M Boguszewski Hans-Peter Lipp Ewelina Knapska Eco-HAB as a fully automated and ecologically relevant assessment of social impairments in mouse models of autism eLife social impairments social behavior mouse models automated testing autism ecological relevance |
| title | Eco-HAB as a fully automated and ecologically relevant assessment of social impairments in mouse models of autism |
| title_full | Eco-HAB as a fully automated and ecologically relevant assessment of social impairments in mouse models of autism |
| title_fullStr | Eco-HAB as a fully automated and ecologically relevant assessment of social impairments in mouse models of autism |
| title_full_unstemmed | Eco-HAB as a fully automated and ecologically relevant assessment of social impairments in mouse models of autism |
| title_short | Eco-HAB as a fully automated and ecologically relevant assessment of social impairments in mouse models of autism |
| title_sort | eco hab as a fully automated and ecologically relevant assessment of social impairments in mouse models of autism |
| topic | social impairments social behavior mouse models automated testing autism ecological relevance |
| url | https://elifesciences.org/articles/19532 |
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