Anti-stress effects of human placenta extract: possible involvement of the oxidative stress system in rats
Abstract Background Human placenta hydrolysate (hPH) has been utilized to improve menopausal, fatigue, liver function. Its high concentration of bioactive substances is known to produce including antioxidant, anti-inflammatory and anti-nociceptive activities. However, its mechanisms of stress-induce...
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| Format: | Article |
| Language: | English |
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BMC
2018-05-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-018-2193-x |
| _version_ | 1818327146115891200 |
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| author | Hyun-Jung Park Hyun Soo Shim Sunyoung Lee Dae Hyun Hahm Hyejung Lee Chang Taek Oh Hae Jung Han Hyi Jeong Ji Insop Shim |
| author_facet | Hyun-Jung Park Hyun Soo Shim Sunyoung Lee Dae Hyun Hahm Hyejung Lee Chang Taek Oh Hae Jung Han Hyi Jeong Ji Insop Shim |
| author_sort | Hyun-Jung Park |
| collection | DOAJ |
| description | Abstract Background Human placenta hydrolysate (hPH) has been utilized to improve menopausal, fatigue, liver function. Its high concentration of bioactive substances is known to produce including antioxidant, anti-inflammatory and anti-nociceptive activities. However, its mechanisms of stress-induced depression remain unknown. Methods The present study examined the effect of hPH on stress-induced depressive behaviors and biochemical parameters in rats. hPH (0.02 ml, 0.2 ml or 1 ml/rat) was injected intravenously 30 min before the daily stress session in male Sprague-Dawley rats exposed to repeated immobilization stress (4 h/day for 7 days). The depressive-like behaviors of all groups were measured by elevated plus maze (EPM) and forced swimming test (FST). After the behavior tests, brain samples of all groups were collected for the analysis of glutathione peroxidase (GPx) and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) staining. Results Treatment with hPH produced a significant decrease of immobility time in the FST compared to the controls. Additionally, hPH treatment elicited a slightly decreasing trend in anxiety behavior on the EPM. Furthermore, hPH increased the level of GPx protein in the hippocampus, and decreased the expression of NADPH-d in the paraventricular nucleus (PVN). Conclusion This study demonstrated that hPH has anti-stress effects via the regulation of nitric oxide (NO) synthase and antioxidant activity in the brain. These results suggest that hPH may be useful in the treatment of stress-related diseases such as chronic fatigue syndrome. |
| first_indexed | 2024-12-13T12:11:38Z |
| format | Article |
| id | doaj.art-67b93f78204e47eda10fde0a044de7d1 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-13T12:11:38Z |
| publishDate | 2018-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-67b93f78204e47eda10fde0a044de7d12022-12-21T23:46:50ZengBMCBMC Complementary and Alternative Medicine1472-68822018-05-011811810.1186/s12906-018-2193-xAnti-stress effects of human placenta extract: possible involvement of the oxidative stress system in ratsHyun-Jung Park0Hyun Soo Shim1Sunyoung Lee2Dae Hyun Hahm3Hyejung Lee4Chang Taek Oh5Hae Jung Han6Hyi Jeong Ji7Insop Shim8Department of Science in Korean Medicine, Graduate School, College of Korean Medicine, Kyung Hee UniversityDepartment of Science in Korean Medicine, Graduate School, College of Korean Medicine, Kyung Hee UniversityDepartment of Science in Korean Medicine, Graduate School, College of Korean Medicine, Kyung Hee UniversityDepartment of Science in Korean Medicine, Graduate School, College of Korean Medicine, Kyung Hee UniversityDepartment of Science in Korean Medicine, Graduate School, College of Korean Medicine, Kyung Hee UniversityBusiness Development Division, Green Cross WellBeingBusiness Development Division, Green Cross WellBeingCorporate Development Division, Green Cross CorpDepartment of Science in Korean Medicine, Graduate School, College of Korean Medicine, Kyung Hee UniversityAbstract Background Human placenta hydrolysate (hPH) has been utilized to improve menopausal, fatigue, liver function. Its high concentration of bioactive substances is known to produce including antioxidant, anti-inflammatory and anti-nociceptive activities. However, its mechanisms of stress-induced depression remain unknown. Methods The present study examined the effect of hPH on stress-induced depressive behaviors and biochemical parameters in rats. hPH (0.02 ml, 0.2 ml or 1 ml/rat) was injected intravenously 30 min before the daily stress session in male Sprague-Dawley rats exposed to repeated immobilization stress (4 h/day for 7 days). The depressive-like behaviors of all groups were measured by elevated plus maze (EPM) and forced swimming test (FST). After the behavior tests, brain samples of all groups were collected for the analysis of glutathione peroxidase (GPx) and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) staining. Results Treatment with hPH produced a significant decrease of immobility time in the FST compared to the controls. Additionally, hPH treatment elicited a slightly decreasing trend in anxiety behavior on the EPM. Furthermore, hPH increased the level of GPx protein in the hippocampus, and decreased the expression of NADPH-d in the paraventricular nucleus (PVN). Conclusion This study demonstrated that hPH has anti-stress effects via the regulation of nitric oxide (NO) synthase and antioxidant activity in the brain. These results suggest that hPH may be useful in the treatment of stress-related diseases such as chronic fatigue syndrome.http://link.springer.com/article/10.1186/s12906-018-2193-xFatigueForced swimming testGlutathione peroxidase (GPx)Human placenta hydrolysate (hPH)Nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d)Immobilization stress |
| spellingShingle | Hyun-Jung Park Hyun Soo Shim Sunyoung Lee Dae Hyun Hahm Hyejung Lee Chang Taek Oh Hae Jung Han Hyi Jeong Ji Insop Shim Anti-stress effects of human placenta extract: possible involvement of the oxidative stress system in rats BMC Complementary and Alternative Medicine Fatigue Forced swimming test Glutathione peroxidase (GPx) Human placenta hydrolysate (hPH) Nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) Immobilization stress |
| title | Anti-stress effects of human placenta extract: possible involvement of the oxidative stress system in rats |
| title_full | Anti-stress effects of human placenta extract: possible involvement of the oxidative stress system in rats |
| title_fullStr | Anti-stress effects of human placenta extract: possible involvement of the oxidative stress system in rats |
| title_full_unstemmed | Anti-stress effects of human placenta extract: possible involvement of the oxidative stress system in rats |
| title_short | Anti-stress effects of human placenta extract: possible involvement of the oxidative stress system in rats |
| title_sort | anti stress effects of human placenta extract possible involvement of the oxidative stress system in rats |
| topic | Fatigue Forced swimming test Glutathione peroxidase (GPx) Human placenta hydrolysate (hPH) Nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) Immobilization stress |
| url | http://link.springer.com/article/10.1186/s12906-018-2193-x |
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