Assessment of high-fat-diet-induced fatty liver in medaka
Fatty liver, which has been continuously becoming more common in a number of patients, is the most common liver disease. For detailed analysis, a useful model for fatty liver is needed and fish are considered as a potential candidate. We assessed through direct observation of the liver, which is the...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Company of Biologists
2018-11-01
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| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/7/11/bio031534 |
| _version_ | 1819138967924113408 |
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| author | Koichi Fujisawa Taro Takami Yumi Fukui Takahiro Nagatomo Issei Saeki Toshihiko Matsumoto Isao Hidaka Naoki Yamamoto Takeshi Okamoto Makoto Furutani-Seiki Isao Sakaida |
| author_facet | Koichi Fujisawa Taro Takami Yumi Fukui Takahiro Nagatomo Issei Saeki Toshihiko Matsumoto Isao Hidaka Naoki Yamamoto Takeshi Okamoto Makoto Furutani-Seiki Isao Sakaida |
| author_sort | Koichi Fujisawa |
| collection | DOAJ |
| description | Fatty liver, which has been continuously becoming more common in a number of patients, is the most common liver disease. For detailed analysis, a useful model for fatty liver is needed and fish are considered as a potential candidate. We assessed through direct observation of the liver, which is the most conventional method for non-invasive analysis of progression in fatty liver. By using transparent medaka (Oryzias latipes), we were able to observe changes in fat deposition in the liver. An analysis of the progression of fatty liver using ultrasound showed a significant increase in echo intensity, which indicates that this is a useful examination method. In addition, we clarified a metabolite profile in the medaka liver fed a high-fat diet (HFD), which had not previously been shown in detail. This medaka model, allowing non-invasive and repetitive assessment, is a useful model for the analysis of diseases that cause fatty liver in which changes in detailed metabolites are identified. |
| first_indexed | 2024-12-22T11:15:11Z |
| format | Article |
| id | doaj.art-67b9cfafc6d347f98ff31e587868ccd3 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-22T11:15:11Z |
| publishDate | 2018-11-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-67b9cfafc6d347f98ff31e587868ccd32022-12-21T18:28:02ZengThe Company of BiologistsBiology Open2046-63902018-11-0171110.1242/bio.031534031534Assessment of high-fat-diet-induced fatty liver in medakaKoichi Fujisawa0Taro Takami1Yumi Fukui2Takahiro Nagatomo3Issei Saeki4Toshihiko Matsumoto5Isao Hidaka6Naoki Yamamoto7Takeshi Okamoto8Makoto Furutani-Seiki9Isao Sakaida10 Center for Regenerative Medicine, Yamaguchi University School of Medicine, 1-1-1 Minami-Kogushi, Ube Yamaguchi 755-8505, Japan Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan Systems Biochemistry in Pathology and Regeneration, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan Center for Regenerative Medicine, Yamaguchi University School of Medicine, 1-1-1 Minami-Kogushi, Ube Yamaguchi 755-8505, Japan Fatty liver, which has been continuously becoming more common in a number of patients, is the most common liver disease. For detailed analysis, a useful model for fatty liver is needed and fish are considered as a potential candidate. We assessed through direct observation of the liver, which is the most conventional method for non-invasive analysis of progression in fatty liver. By using transparent medaka (Oryzias latipes), we were able to observe changes in fat deposition in the liver. An analysis of the progression of fatty liver using ultrasound showed a significant increase in echo intensity, which indicates that this is a useful examination method. In addition, we clarified a metabolite profile in the medaka liver fed a high-fat diet (HFD), which had not previously been shown in detail. This medaka model, allowing non-invasive and repetitive assessment, is a useful model for the analysis of diseases that cause fatty liver in which changes in detailed metabolites are identified.http://bio.biologists.org/content/7/11/bio031534MedakaLiverFatty liverUltrasoundMetabolomics |
| spellingShingle | Koichi Fujisawa Taro Takami Yumi Fukui Takahiro Nagatomo Issei Saeki Toshihiko Matsumoto Isao Hidaka Naoki Yamamoto Takeshi Okamoto Makoto Furutani-Seiki Isao Sakaida Assessment of high-fat-diet-induced fatty liver in medaka Biology Open Medaka Liver Fatty liver Ultrasound Metabolomics |
| title | Assessment of high-fat-diet-induced fatty liver in medaka |
| title_full | Assessment of high-fat-diet-induced fatty liver in medaka |
| title_fullStr | Assessment of high-fat-diet-induced fatty liver in medaka |
| title_full_unstemmed | Assessment of high-fat-diet-induced fatty liver in medaka |
| title_short | Assessment of high-fat-diet-induced fatty liver in medaka |
| title_sort | assessment of high fat diet induced fatty liver in medaka |
| topic | Medaka Liver Fatty liver Ultrasound Metabolomics |
| url | http://bio.biologists.org/content/7/11/bio031534 |
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