Gpr120 mRNA expression in gonadotropes in the mouse pituitary gland is regulated by free fatty acids
GPR120 is a long-chain fatty acid (LCFA) receptor that is specifically expressed in gonadotropes in the anterior pituitary gland in mice. The aim of this study was to investigate whether GPR120 is activated by free fatty acids in the pituitary of mice and mouse immortalized gonadotrope LβT2 cells. F...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Society for Reproduction and Development
2020-02-01
|
| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/66/3/66_2019-166/_pdf/-char/en |
| _version_ | 1797615307666227200 |
|---|---|
| author | Chikaya DEURA Yusuke KIMURA Takumi NONOYAMA Ryutaro MORIYAMA |
| author_facet | Chikaya DEURA Yusuke KIMURA Takumi NONOYAMA Ryutaro MORIYAMA |
| author_sort | Chikaya DEURA |
| collection | DOAJ |
| description | GPR120 is a long-chain fatty acid (LCFA) receptor that is specifically expressed in gonadotropes in the anterior pituitary gland in mice. The aim of this study was to investigate whether GPR120 is activated by free fatty acids in the pituitary of mice and mouse immortalized gonadotrope LβT2 cells. First, the effects of palmitate on GPR120, gonadotropic hormone b-subunits, and GnRH-receptor expression in gonadotropes were investigated in vitro. We observed palmitate-induced an increase in Gpr120 mRNA expression and a decrease in follicle-stimulating hormone b-subunit (Fshb) expression in LβT2 cells. Furthermore, palmitate exposure caused the phosphorylation of ERK1/2 in LβT2 cells, but no significant changes were observed in the expression levels of luteinizing hormone b-subunit (Lhb) and gonadotropin releasing hormone-receptor (Gnrh-r) mRNA and number of GPR120 immunoreactive cells. Next, diurnal variation in Gpr120 mRNA expression in the male mouse pituitary gland was investigated using ad libitum and night-time restricted feeding (active phase from 1900 to 0700 h) treatments. In ad libitum feeding group mice, Gpr120 mRNA expression at 1700 h was transiently higher than that measured at other times, and the peak blood non-esterified fatty acid (NEFA) levels were observed from 1300 to 1500 h. These results were not observed in night-time-restricted feeding group mice. These results suggest that GPR120 is activated by LCFAs to regulate follicle stimulating hormone (FSH) synthesis in the mouse gonadotropes. |
| first_indexed | 2024-03-11T07:24:29Z |
| format | Article |
| id | doaj.art-67ba8eab984a41d7bdcaf6d1f12b771d |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T07:24:29Z |
| publishDate | 2020-02-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-67ba8eab984a41d7bdcaf6d1f12b771d2023-11-17T07:42:39ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002020-02-0166324925410.1262/jrd.2019-166jrdGpr120 mRNA expression in gonadotropes in the mouse pituitary gland is regulated by free fatty acidsChikaya DEURA0Yusuke KIMURA1Takumi NONOYAMA2Ryutaro MORIYAMA3Laboratory of Environmental Physiology, Department of Life Science, School of Science and Engineering, Kindai University, Higashiosaka 577-8502, JapanLaboratory of Environmental Physiology, Department of Life Science, School of Science and Engineering, Kindai University, Higashiosaka 577-8502, JapanLaboratory of Environmental Physiology, Department of Life Science, School of Science and Engineering, Kindai University, Higashiosaka 577-8502, JapanLaboratory of Environmental Physiology, Department of Life Science, School of Science and Engineering, Kindai University, Higashiosaka 577-8502, JapanGPR120 is a long-chain fatty acid (LCFA) receptor that is specifically expressed in gonadotropes in the anterior pituitary gland in mice. The aim of this study was to investigate whether GPR120 is activated by free fatty acids in the pituitary of mice and mouse immortalized gonadotrope LβT2 cells. First, the effects of palmitate on GPR120, gonadotropic hormone b-subunits, and GnRH-receptor expression in gonadotropes were investigated in vitro. We observed palmitate-induced an increase in Gpr120 mRNA expression and a decrease in follicle-stimulating hormone b-subunit (Fshb) expression in LβT2 cells. Furthermore, palmitate exposure caused the phosphorylation of ERK1/2 in LβT2 cells, but no significant changes were observed in the expression levels of luteinizing hormone b-subunit (Lhb) and gonadotropin releasing hormone-receptor (Gnrh-r) mRNA and number of GPR120 immunoreactive cells. Next, diurnal variation in Gpr120 mRNA expression in the male mouse pituitary gland was investigated using ad libitum and night-time restricted feeding (active phase from 1900 to 0700 h) treatments. In ad libitum feeding group mice, Gpr120 mRNA expression at 1700 h was transiently higher than that measured at other times, and the peak blood non-esterified fatty acid (NEFA) levels were observed from 1300 to 1500 h. These results were not observed in night-time-restricted feeding group mice. These results suggest that GPR120 is activated by LCFAs to regulate follicle stimulating hormone (FSH) synthesis in the mouse gonadotropes.https://www.jstage.jst.go.jp/article/jrd/66/3/66_2019-166/_pdf/-char/endiurnal variationfatty acidgonadotropegpr120pituitary |
| spellingShingle | Chikaya DEURA Yusuke KIMURA Takumi NONOYAMA Ryutaro MORIYAMA Gpr120 mRNA expression in gonadotropes in the mouse pituitary gland is regulated by free fatty acids The Journal of Reproduction and Development diurnal variation fatty acid gonadotrope gpr120 pituitary |
| title | Gpr120 mRNA expression in gonadotropes in the mouse pituitary gland is regulated by free fatty acids |
| title_full | Gpr120 mRNA expression in gonadotropes in the mouse pituitary gland is regulated by free fatty acids |
| title_fullStr | Gpr120 mRNA expression in gonadotropes in the mouse pituitary gland is regulated by free fatty acids |
| title_full_unstemmed | Gpr120 mRNA expression in gonadotropes in the mouse pituitary gland is regulated by free fatty acids |
| title_short | Gpr120 mRNA expression in gonadotropes in the mouse pituitary gland is regulated by free fatty acids |
| title_sort | gpr120 mrna expression in gonadotropes in the mouse pituitary gland is regulated by free fatty acids |
| topic | diurnal variation fatty acid gonadotrope gpr120 pituitary |
| url | https://www.jstage.jst.go.jp/article/jrd/66/3/66_2019-166/_pdf/-char/en |
| work_keys_str_mv | AT chikayadeura gpr120mrnaexpressioningonadotropesinthemousepituitaryglandisregulatedbyfreefattyacids AT yusukekimura gpr120mrnaexpressioningonadotropesinthemousepituitaryglandisregulatedbyfreefattyacids AT takuminonoyama gpr120mrnaexpressioningonadotropesinthemousepituitaryglandisregulatedbyfreefattyacids AT ryutaromoriyama gpr120mrnaexpressioningonadotropesinthemousepituitaryglandisregulatedbyfreefattyacids |