A Luminescence-Based Human TRPV1 Assay System for Quantifying Pungency in Spicy Foods
The quantitation of pungency is difficult to achieve using sensory tests because of persistence, accumulation, and desensitization to the perception of pungency. Transient receptor vanilloid 1 (TRPV1), which is a chemosensory receptor, plays a pivotal role in the perception of many pungent compounds...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-01-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/10/1/151 |
| _version_ | 1797412335186345984 |
|---|---|
| author | Minami Matsuyama Yuko Terada Toyomi Yamazaki-Ito Keisuke Ito |
| author_facet | Minami Matsuyama Yuko Terada Toyomi Yamazaki-Ito Keisuke Ito |
| author_sort | Minami Matsuyama |
| collection | DOAJ |
| description | The quantitation of pungency is difficult to achieve using sensory tests because of persistence, accumulation, and desensitization to the perception of pungency. Transient receptor vanilloid 1 (TRPV1), which is a chemosensory receptor, plays a pivotal role in the perception of many pungent compounds, suggesting that the activity of this receptor might be useful as an index for pungency evaluation. Although Ca<sup>2+</sup>-sensitive fluorescence dyes are commonly used for measuring human TRPV1 (hTRPV1) activity, their application is limited, as foods often contain fluorescent substances that interfere with the fluorescent signals. This study aims to design a new pungency evaluation system using hTRPV1. Instead of employing a fluorescent probe as the Ca<sup>2+</sup> indicator, this assay system uses the luminescent protein aequorin. The luminescence assay successfully evaluated the hTRPV1 activity in foods without purification, even for those containing fluorescent substances. The hTRPV1 activity in food samples correlated strongly with the pungency intensity obtained by the human sensory test. This luminescence-based hTRPV1 assay system will be a powerful tool for objectively quantifying the pungency of spicy foods in both laboratory and industrial settings. |
| first_indexed | 2024-03-09T05:01:06Z |
| format | Article |
| id | doaj.art-67bb21e6af5946b89f22501029cc2223 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T05:01:06Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-67bb21e6af5946b89f22501029cc22232023-12-03T13:00:07ZengMDPI AGFoods2304-81582021-01-0110115110.3390/foods10010151A Luminescence-Based Human TRPV1 Assay System for Quantifying Pungency in Spicy FoodsMinami Matsuyama0Yuko Terada1Toyomi Yamazaki-Ito2Keisuke Ito3Department of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, JapanDepartment of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, JapanDepartment of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, JapanDepartment of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, JapanThe quantitation of pungency is difficult to achieve using sensory tests because of persistence, accumulation, and desensitization to the perception of pungency. Transient receptor vanilloid 1 (TRPV1), which is a chemosensory receptor, plays a pivotal role in the perception of many pungent compounds, suggesting that the activity of this receptor might be useful as an index for pungency evaluation. Although Ca<sup>2+</sup>-sensitive fluorescence dyes are commonly used for measuring human TRPV1 (hTRPV1) activity, their application is limited, as foods often contain fluorescent substances that interfere with the fluorescent signals. This study aims to design a new pungency evaluation system using hTRPV1. Instead of employing a fluorescent probe as the Ca<sup>2+</sup> indicator, this assay system uses the luminescent protein aequorin. The luminescence assay successfully evaluated the hTRPV1 activity in foods without purification, even for those containing fluorescent substances. The hTRPV1 activity in food samples correlated strongly with the pungency intensity obtained by the human sensory test. This luminescence-based hTRPV1 assay system will be a powerful tool for objectively quantifying the pungency of spicy foods in both laboratory and industrial settings.https://www.mdpi.com/2304-8158/10/1/151pungency evaluationTRPV1luminescence assayspicy foodsflavor evaluation |
| spellingShingle | Minami Matsuyama Yuko Terada Toyomi Yamazaki-Ito Keisuke Ito A Luminescence-Based Human TRPV1 Assay System for Quantifying Pungency in Spicy Foods Foods pungency evaluation TRPV1 luminescence assay spicy foods flavor evaluation |
| title | A Luminescence-Based Human TRPV1 Assay System for Quantifying Pungency in Spicy Foods |
| title_full | A Luminescence-Based Human TRPV1 Assay System for Quantifying Pungency in Spicy Foods |
| title_fullStr | A Luminescence-Based Human TRPV1 Assay System for Quantifying Pungency in Spicy Foods |
| title_full_unstemmed | A Luminescence-Based Human TRPV1 Assay System for Quantifying Pungency in Spicy Foods |
| title_short | A Luminescence-Based Human TRPV1 Assay System for Quantifying Pungency in Spicy Foods |
| title_sort | luminescence based human trpv1 assay system for quantifying pungency in spicy foods |
| topic | pungency evaluation TRPV1 luminescence assay spicy foods flavor evaluation |
| url | https://www.mdpi.com/2304-8158/10/1/151 |
| work_keys_str_mv | AT minamimatsuyama aluminescencebasedhumantrpv1assaysystemforquantifyingpungencyinspicyfoods AT yukoterada aluminescencebasedhumantrpv1assaysystemforquantifyingpungencyinspicyfoods AT toyomiyamazakiito aluminescencebasedhumantrpv1assaysystemforquantifyingpungencyinspicyfoods AT keisukeito aluminescencebasedhumantrpv1assaysystemforquantifyingpungencyinspicyfoods AT minamimatsuyama luminescencebasedhumantrpv1assaysystemforquantifyingpungencyinspicyfoods AT yukoterada luminescencebasedhumantrpv1assaysystemforquantifyingpungencyinspicyfoods AT toyomiyamazakiito luminescencebasedhumantrpv1assaysystemforquantifyingpungencyinspicyfoods AT keisukeito luminescencebasedhumantrpv1assaysystemforquantifyingpungencyinspicyfoods |