The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression.
BACKGROUND:Activator protein-1 (AP-1) is a mediator of BMP or FGF signaling during Xenopus embryogenesis. However, specific role of AP-1 in activin signaling has not been elucidated during vertebrate development. METHODOLOGY/PRINCIPAL FINDINGS:We provide new evidence showing that overexpression of h...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3146467?pdf=render |
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| author | Sung-Young Lee Jaeho Yoon Hyun-Shik Lee Yoo-Seok Hwang Sang-Wook Cha Chul-Ho Jeong Jong-Il Kim Jae-Bong Park Jae-Yong Lee SungChan Kim Mae Ja Park Zigang Dong Jaebong Kim |
| author_facet | Sung-Young Lee Jaeho Yoon Hyun-Shik Lee Yoo-Seok Hwang Sang-Wook Cha Chul-Ho Jeong Jong-Il Kim Jae-Bong Park Jae-Yong Lee SungChan Kim Mae Ja Park Zigang Dong Jaebong Kim |
| author_sort | Sung-Young Lee |
| collection | DOAJ |
| description | BACKGROUND:Activator protein-1 (AP-1) is a mediator of BMP or FGF signaling during Xenopus embryogenesis. However, specific role of AP-1 in activin signaling has not been elucidated during vertebrate development. METHODOLOGY/PRINCIPAL FINDINGS:We provide new evidence showing that overexpression of heterodimeric AP-1 comprised of c-jun and c-fos (AP-1(c-Jun/c-Fos)) induces the expression of BMP-antagonizing organizer genes (noggin, chordin and goosecoid) that were normally expressed by high dose of activin. AP-1(c-Jun/c-Fos) enhanced the promoter activities of organizer genes but reduced that of PV.1, a BMP4-response gene. A loss of function study clearly demonstrated that AP-1(c-Jun/c-Fos) is required for the activin-induced organizer and neural gene expression. Moreover, physical interaction of AP-1(c-Jun/c-Fos) and Smad3 cooperatively enhanced the transcriptional activity of goosecoid via direct binding on this promoter. Interestingly, Smad3 mutants at c-Jun binding site failed in regulation of organizer genes, indicating that these physical interactions are specifically necessary for the expression of organizer genes. CONCLUSIONS/SIGNIFICANCE:AP-1(c-Jun/c-Fos) plays a specific role in organizer gene expression in downstream of activin signal during early Xenopus embryogenesis. |
| first_indexed | 2024-12-23T14:44:11Z |
| format | Article |
| id | doaj.art-67bb874c3bd942d0bac7797164de3c09 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T14:44:11Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-67bb874c3bd942d0bac7797164de3c092022-12-21T17:43:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0167e2179610.1371/journal.pone.0021796The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression.Sung-Young LeeJaeho YoonHyun-Shik LeeYoo-Seok HwangSang-Wook ChaChul-Ho JeongJong-Il KimJae-Bong ParkJae-Yong LeeSungChan KimMae Ja ParkZigang DongJaebong KimBACKGROUND:Activator protein-1 (AP-1) is a mediator of BMP or FGF signaling during Xenopus embryogenesis. However, specific role of AP-1 in activin signaling has not been elucidated during vertebrate development. METHODOLOGY/PRINCIPAL FINDINGS:We provide new evidence showing that overexpression of heterodimeric AP-1 comprised of c-jun and c-fos (AP-1(c-Jun/c-Fos)) induces the expression of BMP-antagonizing organizer genes (noggin, chordin and goosecoid) that were normally expressed by high dose of activin. AP-1(c-Jun/c-Fos) enhanced the promoter activities of organizer genes but reduced that of PV.1, a BMP4-response gene. A loss of function study clearly demonstrated that AP-1(c-Jun/c-Fos) is required for the activin-induced organizer and neural gene expression. Moreover, physical interaction of AP-1(c-Jun/c-Fos) and Smad3 cooperatively enhanced the transcriptional activity of goosecoid via direct binding on this promoter. Interestingly, Smad3 mutants at c-Jun binding site failed in regulation of organizer genes, indicating that these physical interactions are specifically necessary for the expression of organizer genes. CONCLUSIONS/SIGNIFICANCE:AP-1(c-Jun/c-Fos) plays a specific role in organizer gene expression in downstream of activin signal during early Xenopus embryogenesis.http://europepmc.org/articles/PMC3146467?pdf=render |
| spellingShingle | Sung-Young Lee Jaeho Yoon Hyun-Shik Lee Yoo-Seok Hwang Sang-Wook Cha Chul-Ho Jeong Jong-Il Kim Jae-Bong Park Jae-Yong Lee SungChan Kim Mae Ja Park Zigang Dong Jaebong Kim The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression. PLoS ONE |
| title | The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression. |
| title_full | The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression. |
| title_fullStr | The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression. |
| title_full_unstemmed | The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression. |
| title_short | The function of heterodimeric AP-1 comprised of c-Jun and c-Fos in activin mediated Spemann organizer gene expression. |
| title_sort | function of heterodimeric ap 1 comprised of c jun and c fos in activin mediated spemann organizer gene expression |
| url | http://europepmc.org/articles/PMC3146467?pdf=render |
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