QSAR study and the hydrolysis activity prediction of three alkaline lipases from different lipase-producing microorganisms
<p>Abstract</p> <p>The hydrolysis activities of three alkaline lipases, L-A1, L-A2 and L-A3 secreted by different lipase-producing microorganisms isolated from the Bay of Bohai, P. R. China were characterized with 16 kinds of esters. It was found that all the lipases have the abili...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-09-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://www.lipidworld.com/content/11/1/124 |
| _version_ | 1811244508942172160 |
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| author | Wang Haikuan Wang Xiaojie Li Xiaolu Zhang Yehong Dai Yujie Guo Changlu Zheng Heng |
| author_facet | Wang Haikuan Wang Xiaojie Li Xiaolu Zhang Yehong Dai Yujie Guo Changlu Zheng Heng |
| author_sort | Wang Haikuan |
| collection | DOAJ |
| description | <p>Abstract</p> <p>The hydrolysis activities of three alkaline lipases, L-A1, L-A2 and L-A3 secreted by different lipase-producing microorganisms isolated from the Bay of Bohai, P. R. China were characterized with 16 kinds of esters. It was found that all the lipases have the ability to catalyze the hydrolysis of the glycerides, methyl esters, ethyl esters, especially for triglycerides, which shows that they have broad substrate spectra, and this property is very important for them to be used in detergent industry. Three QSAR models were built for L-A1, L-A2 and L-A3 respectively with GFA using Discovery studio 2.1. The models equations 1, 2 and 3 can explain 95.80%, 97.45% and 97.09% of the variances (<it>R</it><sup><it>2</it></sup><sub><it>adj</it></sub>) respectively while they could predict 95.44%, 89.61% and 93.41% of the variances (<it>R</it><sup><it>2</it></sup><sub><it>cv</it></sub>) respectively. With these models the hydrolysis activities of these lipases to mixed esters were predicted and the result showed that the predicted values are in good agreement with the measured values, which indicates that this method can be used as a simple tool to predict the lipase activities for single or mixed esters.</p> |
| first_indexed | 2024-04-12T14:26:19Z |
| format | Article |
| id | doaj.art-67bdaa198f204c0aa7f21e9e880aece4 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-04-12T14:26:19Z |
| publishDate | 2012-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-67bdaa198f204c0aa7f21e9e880aece42022-12-22T03:29:25ZengBMCLipids in Health and Disease1476-511X2012-09-0111112410.1186/1476-511X-11-124QSAR study and the hydrolysis activity prediction of three alkaline lipases from different lipase-producing microorganismsWang HaikuanWang XiaojieLi XiaoluZhang YehongDai YujieGuo ChangluZheng Heng<p>Abstract</p> <p>The hydrolysis activities of three alkaline lipases, L-A1, L-A2 and L-A3 secreted by different lipase-producing microorganisms isolated from the Bay of Bohai, P. R. China were characterized with 16 kinds of esters. It was found that all the lipases have the ability to catalyze the hydrolysis of the glycerides, methyl esters, ethyl esters, especially for triglycerides, which shows that they have broad substrate spectra, and this property is very important for them to be used in detergent industry. Three QSAR models were built for L-A1, L-A2 and L-A3 respectively with GFA using Discovery studio 2.1. The models equations 1, 2 and 3 can explain 95.80%, 97.45% and 97.09% of the variances (<it>R</it><sup><it>2</it></sup><sub><it>adj</it></sub>) respectively while they could predict 95.44%, 89.61% and 93.41% of the variances (<it>R</it><sup><it>2</it></sup><sub><it>cv</it></sub>) respectively. With these models the hydrolysis activities of these lipases to mixed esters were predicted and the result showed that the predicted values are in good agreement with the measured values, which indicates that this method can be used as a simple tool to predict the lipase activities for single or mixed esters.</p>http://www.lipidworld.com/content/11/1/124QSARGFAEsterLipase activityVegetable oils |
| spellingShingle | Wang Haikuan Wang Xiaojie Li Xiaolu Zhang Yehong Dai Yujie Guo Changlu Zheng Heng QSAR study and the hydrolysis activity prediction of three alkaline lipases from different lipase-producing microorganisms Lipids in Health and Disease QSAR GFA Ester Lipase activity Vegetable oils |
| title | QSAR study and the hydrolysis activity prediction of three alkaline lipases from different lipase-producing microorganisms |
| title_full | QSAR study and the hydrolysis activity prediction of three alkaline lipases from different lipase-producing microorganisms |
| title_fullStr | QSAR study and the hydrolysis activity prediction of three alkaline lipases from different lipase-producing microorganisms |
| title_full_unstemmed | QSAR study and the hydrolysis activity prediction of three alkaline lipases from different lipase-producing microorganisms |
| title_short | QSAR study and the hydrolysis activity prediction of three alkaline lipases from different lipase-producing microorganisms |
| title_sort | qsar study and the hydrolysis activity prediction of three alkaline lipases from different lipase producing microorganisms |
| topic | QSAR GFA Ester Lipase activity Vegetable oils |
| url | http://www.lipidworld.com/content/11/1/124 |
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