Identification of chemosensory receptor genes in Manduca sexta and knockdown by RNA interference
<p>Abstract</p> <p>Background</p> <p>Insects detect environmental chemicals via a large and rapidly evolving family of chemosensory receptor proteins. Although our understanding of the molecular genetic basis for Drosophila chemoreception has increased enormously in the...
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| Format: | Article |
| Language: | English |
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BMC
2012-05-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/13/211 |
| _version_ | 1818144784042164224 |
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| author | Howlett Natalie Dauber Katherine L Shukla Aditi Morton Brian Glendinning John I Brent Elyssa Gleason Caroline Islam Fahmida Izquierdo Denisse Sanghavi Sweta Afroz Anika Aslam Aanam Barbaro Marissa Blutstein Rebekah Borovka Margarita Desire Brianna Elikhis Ayala Fan Qing Hoffman Katherine Huang Amy Keefe Dominique Lopatin Sarah Miller Samara Patel Priyata Rizzini Danielle Robinson Alyssa Rokins Karimah Turlik Aneta Mansfield Jennifer H |
| author_facet | Howlett Natalie Dauber Katherine L Shukla Aditi Morton Brian Glendinning John I Brent Elyssa Gleason Caroline Islam Fahmida Izquierdo Denisse Sanghavi Sweta Afroz Anika Aslam Aanam Barbaro Marissa Blutstein Rebekah Borovka Margarita Desire Brianna Elikhis Ayala Fan Qing Hoffman Katherine Huang Amy Keefe Dominique Lopatin Sarah Miller Samara Patel Priyata Rizzini Danielle Robinson Alyssa Rokins Karimah Turlik Aneta Mansfield Jennifer H |
| author_sort | Howlett Natalie |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Insects detect environmental chemicals via a large and rapidly evolving family of chemosensory receptor proteins. Although our understanding of the molecular genetic basis for Drosophila chemoreception has increased enormously in the last decade, similar understanding in other insects remains limited. The tobacco hornworm, Manduca sexta, has long been an important model for insect chemosensation, particularly from ecological, behavioral, and physiological standpoints. It is also a major agricultural pest on solanaceous crops. However, little sequence information and lack of genetic tools has prevented molecular genetic analysis in this species. The ability to connect molecular genetic mechanisms, including potential lineage-specific changes in chemosensory genes, to ecologically relevant behaviors and specializations in M. sexta would be greatly beneficial.</p> <p>Results</p> <p>Here, we sequenced transcriptomes from adult and larval chemosensory tissues and identified chemosensory genes based on sequence homology. We also used dsRNA feeding as a method to induce RNA interference in larval chemosensory tissues.</p> <p>Conclusions</p> <p>We report identification of new chemosensory receptor genes including 17 novel odorant receptors and one novel gustatory receptor. Further, we demonstrate that systemic RNA interference can be used in larval olfactory neurons to reduce expression of chemosensory receptor transcripts. Together, our results further the development of M. sexta as a model for functional analysis of insect chemosensation.</p> |
| first_indexed | 2024-12-11T11:53:04Z |
| format | Article |
| id | doaj.art-67c222ddd4ef43d68989c536c819d8fa |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-11T11:53:04Z |
| publishDate | 2012-05-01 |
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| series | BMC Genomics |
| spelling | doaj.art-67c222ddd4ef43d68989c536c819d8fa2022-12-22T01:08:17ZengBMCBMC Genomics1471-21642012-05-0113121110.1186/1471-2164-13-211Identification of chemosensory receptor genes in Manduca sexta and knockdown by RNA interferenceHowlett NatalieDauber Katherine LShukla AditiMorton BrianGlendinning John IBrent ElyssaGleason CarolineIslam FahmidaIzquierdo DenisseSanghavi SwetaAfroz AnikaAslam AanamBarbaro MarissaBlutstein RebekahBorovka MargaritaDesire BriannaElikhis AyalaFan QingHoffman KatherineHuang AmyKeefe DominiqueLopatin SarahMiller SamaraPatel PriyataRizzini DanielleRobinson AlyssaRokins KarimahTurlik AnetaMansfield Jennifer H<p>Abstract</p> <p>Background</p> <p>Insects detect environmental chemicals via a large and rapidly evolving family of chemosensory receptor proteins. Although our understanding of the molecular genetic basis for Drosophila chemoreception has increased enormously in the last decade, similar understanding in other insects remains limited. The tobacco hornworm, Manduca sexta, has long been an important model for insect chemosensation, particularly from ecological, behavioral, and physiological standpoints. It is also a major agricultural pest on solanaceous crops. However, little sequence information and lack of genetic tools has prevented molecular genetic analysis in this species. The ability to connect molecular genetic mechanisms, including potential lineage-specific changes in chemosensory genes, to ecologically relevant behaviors and specializations in M. sexta would be greatly beneficial.</p> <p>Results</p> <p>Here, we sequenced transcriptomes from adult and larval chemosensory tissues and identified chemosensory genes based on sequence homology. We also used dsRNA feeding as a method to induce RNA interference in larval chemosensory tissues.</p> <p>Conclusions</p> <p>We report identification of new chemosensory receptor genes including 17 novel odorant receptors and one novel gustatory receptor. Further, we demonstrate that systemic RNA interference can be used in larval olfactory neurons to reduce expression of chemosensory receptor transcripts. Together, our results further the development of M. sexta as a model for functional analysis of insect chemosensation.</p>http://www.biomedcentral.com/1471-2164/13/211 |
| spellingShingle | Howlett Natalie Dauber Katherine L Shukla Aditi Morton Brian Glendinning John I Brent Elyssa Gleason Caroline Islam Fahmida Izquierdo Denisse Sanghavi Sweta Afroz Anika Aslam Aanam Barbaro Marissa Blutstein Rebekah Borovka Margarita Desire Brianna Elikhis Ayala Fan Qing Hoffman Katherine Huang Amy Keefe Dominique Lopatin Sarah Miller Samara Patel Priyata Rizzini Danielle Robinson Alyssa Rokins Karimah Turlik Aneta Mansfield Jennifer H Identification of chemosensory receptor genes in Manduca sexta and knockdown by RNA interference BMC Genomics |
| title | Identification of chemosensory receptor genes in Manduca sexta and knockdown by RNA interference |
| title_full | Identification of chemosensory receptor genes in Manduca sexta and knockdown by RNA interference |
| title_fullStr | Identification of chemosensory receptor genes in Manduca sexta and knockdown by RNA interference |
| title_full_unstemmed | Identification of chemosensory receptor genes in Manduca sexta and knockdown by RNA interference |
| title_short | Identification of chemosensory receptor genes in Manduca sexta and knockdown by RNA interference |
| title_sort | identification of chemosensory receptor genes in manduca sexta and knockdown by rna interference |
| url | http://www.biomedcentral.com/1471-2164/13/211 |
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