A simple and rapid method to assay triacylglycerol in cells and tissues
We have developed a reliable, rapid, and economical assay for the quantification of triacylglycerol (TG) in cells and animal tissues. In a few hours, this assay quantifies microgram amounts of TG from tens or even hundreds of samples. The protocol includes an organic extraction to partition TG away...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2007-11-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520424514 |
| _version_ | 1818726899883442176 |
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| author | Danielle M. Schwartz Nathan E. Wolins |
| author_facet | Danielle M. Schwartz Nathan E. Wolins |
| author_sort | Danielle M. Schwartz |
| collection | DOAJ |
| description | We have developed a reliable, rapid, and economical assay for the quantification of triacylglycerol (TG) in cells and animal tissues. In a few hours, this assay quantifies microgram amounts of TG from tens or even hundreds of samples. The protocol includes an organic extraction to partition TG away from proteins and other hydrophilic molecules found in cells and tissues that may interfere with the colorimetric enzyme-linked TG detection method. In addition, this assay is economical, as no expensive reagents, supplies, or equipment are needed. Another benefit of this assay is that it does not require environmentally unfriendly halogenated solvents. |
| first_indexed | 2024-12-17T22:05:32Z |
| format | Article |
| id | doaj.art-67c302478f134720acb9b5b910c7f49c |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T22:05:32Z |
| publishDate | 2007-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-67c302478f134720acb9b5b910c7f49c2022-12-21T21:30:52ZengElsevierJournal of Lipid Research0022-22752007-11-01481125142520A simple and rapid method to assay triacylglycerol in cells and tissuesDanielle M. Schwartz0Nathan E. Wolins1Division of Nutritional Science, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110Division of Nutritional Science, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110We have developed a reliable, rapid, and economical assay for the quantification of triacylglycerol (TG) in cells and animal tissues. In a few hours, this assay quantifies microgram amounts of TG from tens or even hundreds of samples. The protocol includes an organic extraction to partition TG away from proteins and other hydrophilic molecules found in cells and tissues that may interfere with the colorimetric enzyme-linked TG detection method. In addition, this assay is economical, as no expensive reagents, supplies, or equipment are needed. Another benefit of this assay is that it does not require environmentally unfriendly halogenated solvents.http://www.sciencedirect.com/science/article/pii/S0022227520424514fatty acidsfatlipidscreenacyl-glycerol |
| spellingShingle | Danielle M. Schwartz Nathan E. Wolins A simple and rapid method to assay triacylglycerol in cells and tissues Journal of Lipid Research fatty acids fat lipid screen acyl-glycerol |
| title | A simple and rapid method to assay triacylglycerol in cells and tissues |
| title_full | A simple and rapid method to assay triacylglycerol in cells and tissues |
| title_fullStr | A simple and rapid method to assay triacylglycerol in cells and tissues |
| title_full_unstemmed | A simple and rapid method to assay triacylglycerol in cells and tissues |
| title_short | A simple and rapid method to assay triacylglycerol in cells and tissues |
| title_sort | simple and rapid method to assay triacylglycerol in cells and tissues |
| topic | fatty acids fat lipid screen acyl-glycerol |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520424514 |
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